Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models
There are numerous applications of graphene in biomedicine and they can be classified into several main areas: delivery systems, sensors, tissue engineering and biological agents. The growing biomedical field of applications of graphene and its derivates raises questions regarding their toxicity. We...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/15/4250 |
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| author | Sławomir Jaworski Barbara Strojny-Cieślak Mateusz Wierzbicki Marta Kutwin Ewa Sawosz Maciej Kamaszewski Arkadiusz Matuszewski Malwina Sosnowska Jarosław Szczepaniak Karolina Daniluk Agata Lange Michał Pruchniewski Katarzyna Zawadzka Maciej Łojkowski Andre Chwalibog |
| author_facet | Sławomir Jaworski Barbara Strojny-Cieślak Mateusz Wierzbicki Marta Kutwin Ewa Sawosz Maciej Kamaszewski Arkadiusz Matuszewski Malwina Sosnowska Jarosław Szczepaniak Karolina Daniluk Agata Lange Michał Pruchniewski Katarzyna Zawadzka Maciej Łojkowski Andre Chwalibog |
| author_sort | Sławomir Jaworski |
| collection | DOAJ |
| description | There are numerous applications of graphene in biomedicine and they can be classified into several main areas: delivery systems, sensors, tissue engineering and biological agents. The growing biomedical field of applications of graphene and its derivates raises questions regarding their toxicity. We will demonstrate an analysis of the toxicity of two forms of graphene using four various biological models: zebrafish (<i>Danio rerio</i>) embryo, duckweed (<i>Lemna minor</i>), human HS-5 cells and bacteria (<i>Staphylococcus aureus</i>). The toxicity of pristine graphene (PG) and graphene oxide (GO) was tested at concentrations of 5, 10, 20, 50 and 100 µg/mL. Higher toxicity was noted after administration of high doses of PG and GO in all tested biological models. Hydrophilic GO shows greater toxicity to biological models living in the entire volume of the culture medium (zebrafish, duckweed, <i>S. aureus</i>). PG showed the highest toxicity to adherent cells growing on the bottom of the culture plates—human HS-5 cells. The differences in toxicity between the tested graphene materials result from their physicochemical properties and the model used. Dose-dependent toxicity has been demonstrated with both forms of graphene. |
| first_indexed | 2024-03-10T09:12:07Z |
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| id | doaj.art-7acf56ac67c146a199f9c2065c2a8be4 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T09:12:07Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-7acf56ac67c146a199f9c2065c2a8be42023-11-22T05:53:57ZengMDPI AGMaterials1996-19442021-07-011415425010.3390/ma14154250Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological ModelsSławomir Jaworski0Barbara Strojny-Cieślak1Mateusz Wierzbicki2Marta Kutwin3Ewa Sawosz4Maciej Kamaszewski5Arkadiusz Matuszewski6Malwina Sosnowska7Jarosław Szczepaniak8Karolina Daniluk9Agata Lange10Michał Pruchniewski11Katarzyna Zawadzka12Maciej Łojkowski13Andre Chwalibog14Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Ichthyology and Biotechnology in Aquaculture, Institute of Animal Sciences, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Animal Breeding, Institute of Animal Sciences, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, PolandFaculty of Materials Science and Engineering, Warsaw University of Technology, Wołoska 141, 02-507 Warsaw, PolandDepartment of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, 1870 Frederiksberg, DenmarkThere are numerous applications of graphene in biomedicine and they can be classified into several main areas: delivery systems, sensors, tissue engineering and biological agents. The growing biomedical field of applications of graphene and its derivates raises questions regarding their toxicity. We will demonstrate an analysis of the toxicity of two forms of graphene using four various biological models: zebrafish (<i>Danio rerio</i>) embryo, duckweed (<i>Lemna minor</i>), human HS-5 cells and bacteria (<i>Staphylococcus aureus</i>). The toxicity of pristine graphene (PG) and graphene oxide (GO) was tested at concentrations of 5, 10, 20, 50 and 100 µg/mL. Higher toxicity was noted after administration of high doses of PG and GO in all tested biological models. Hydrophilic GO shows greater toxicity to biological models living in the entire volume of the culture medium (zebrafish, duckweed, <i>S. aureus</i>). PG showed the highest toxicity to adherent cells growing on the bottom of the culture plates—human HS-5 cells. The differences in toxicity between the tested graphene materials result from their physicochemical properties and the model used. Dose-dependent toxicity has been demonstrated with both forms of graphene.https://www.mdpi.com/1996-1944/14/15/4250graphenegraphene oxidetoxicitybiological models |
| spellingShingle | Sławomir Jaworski Barbara Strojny-Cieślak Mateusz Wierzbicki Marta Kutwin Ewa Sawosz Maciej Kamaszewski Arkadiusz Matuszewski Malwina Sosnowska Jarosław Szczepaniak Karolina Daniluk Agata Lange Michał Pruchniewski Katarzyna Zawadzka Maciej Łojkowski Andre Chwalibog Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models Materials graphene graphene oxide toxicity biological models |
| title | Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models |
| title_full | Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models |
| title_fullStr | Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models |
| title_full_unstemmed | Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models |
| title_short | Comparison of the Toxicity of Pristine Graphene and Graphene Oxide, Using Four Biological Models |
| title_sort | comparison of the toxicity of pristine graphene and graphene oxide using four biological models |
| topic | graphene graphene oxide toxicity biological models |
| url | https://www.mdpi.com/1996-1944/14/15/4250 |
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