Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1
Abstract Background Although influenza A virus (IAV) employs diverse strategies to evade IFN responses by inhibiting the synthesis of IFN, how IAV regulates signaling downstream of IFN is incompletely understood. Methods In this study, we used Western blot-based protein analysis coupled with RT-qPCR...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01348-4 |
| _version_ | 1811313949563420672 |
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| author | Yinping Du Fan Yang Qiuxia Wang Nuo Xu Yizhang Xie Sujuan Chen Tao Qin Daxin Peng |
| author_facet | Yinping Du Fan Yang Qiuxia Wang Nuo Xu Yizhang Xie Sujuan Chen Tao Qin Daxin Peng |
| author_sort | Yinping Du |
| collection | DOAJ |
| description | Abstract Background Although influenza A virus (IAV) employs diverse strategies to evade IFN responses by inhibiting the synthesis of IFN, how IAV regulates signaling downstream of IFN is incompletely understood. Methods In this study, we used Western blot-based protein analysis coupled with RT-qPCR, overexpression and RNA interference to investigate the regulation of JAK1 by IAV infection. Results The results indicated that JAK1 was ubiquitinated and degraded, resulting in inhibition of type I and type II IFN responses, demonstrating that IAV antagonizes the IFN-activated JAK/STAT signaling pathway by inducing the degradation of JAK1. Furthermore. IAV infection upregulated the suppressor of cytokine signaling (SOCS) protein SOCS1, and SOCS1 mediated the ubiquitination and degradation of JAK1. Conclusion Collectively, our findings suggest that IAV infection induces SOCS1 expression to promote JAK1 degradation, which in turn inhibits host innate immune responses. |
| first_indexed | 2024-04-13T11:03:24Z |
| format | Article |
| id | doaj.art-7acf80a924b84b0294421fe5e52fd507 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-13T11:03:24Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-7acf80a924b84b0294421fe5e52fd5072022-12-22T02:49:21ZengBMCVirology Journal1743-422X2020-06-0117111010.1186/s12985-020-01348-4Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1Yinping Du0Fan Yang1Qiuxia Wang2Nuo Xu3Yizhang Xie4Sujuan Chen5Tao Qin6Daxin Peng7College of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityAbstract Background Although influenza A virus (IAV) employs diverse strategies to evade IFN responses by inhibiting the synthesis of IFN, how IAV regulates signaling downstream of IFN is incompletely understood. Methods In this study, we used Western blot-based protein analysis coupled with RT-qPCR, overexpression and RNA interference to investigate the regulation of JAK1 by IAV infection. Results The results indicated that JAK1 was ubiquitinated and degraded, resulting in inhibition of type I and type II IFN responses, demonstrating that IAV antagonizes the IFN-activated JAK/STAT signaling pathway by inducing the degradation of JAK1. Furthermore. IAV infection upregulated the suppressor of cytokine signaling (SOCS) protein SOCS1, and SOCS1 mediated the ubiquitination and degradation of JAK1. Conclusion Collectively, our findings suggest that IAV infection induces SOCS1 expression to promote JAK1 degradation, which in turn inhibits host innate immune responses.http://link.springer.com/article/10.1186/s12985-020-01348-4IAVJAK1IFNSOCS1Degradation |
| spellingShingle | Yinping Du Fan Yang Qiuxia Wang Nuo Xu Yizhang Xie Sujuan Chen Tao Qin Daxin Peng Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1 Virology Journal IAV JAK1 IFN SOCS1 Degradation |
| title | Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1 |
| title_full | Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1 |
| title_fullStr | Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1 |
| title_full_unstemmed | Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1 |
| title_short | Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1 |
| title_sort | influenza a virus antagonizes type i and type ii interferon responses via socs1 dependent ubiquitination and degradation of jak1 |
| topic | IAV JAK1 IFN SOCS1 Degradation |
| url | http://link.springer.com/article/10.1186/s12985-020-01348-4 |
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