Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells
Background Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with p...
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| Format: | Article |
| Language: | English |
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SciELO
2013-05-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100308&lng=en&tlng=en |
| _version_ | 1818334667204460544 |
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| author | Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez |
| author_facet | Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez |
| author_sort | Heidi I Monroy-Estrada |
| collection | DOAJ |
| description | Background Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells. Results Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under specific conditions. Exposure to CV at 50 μgmL-1 induced a reduction of approximately 50% in cell viability, while a similar cytotoxic effect was observed when cell culture was exposed to F1 at 25 μgmL -1 or F2 at 50 μgmL-1. The cell culture exposure to F1 (10 μgmL-1) fraction combined with cisplatine (25 μM) provoked a decrease in MTT reduction until 65.57% while F2 (25 μgmL-1) fraction combined with cisplatin (10 μM) provoked a decrease in MTT reduction of 72.55%. Conclusions The F1 fraction had the greatest effect on the lung adenocarcinoma cell line compared with CV and F2. The combination of antineoplastic drugs and sea anemone toxins might allow a reduction of chemotherapeutic doses and thus mitigate side effects. |
| first_indexed | 2024-12-13T14:11:10Z |
| format | Article |
| id | doaj.art-7ad104b738844330a4a350a51b895df4 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-13T14:11:10Z |
| publishDate | 2013-05-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-7ad104b738844330a4a350a51b895df42022-12-21T23:42:28ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992013-05-0119010.1186/1678-9199-19-12S1678-91992013000100308Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cellsHeidi I Monroy-EstradaYolanda I ChirinoIrma E Soria-MercadoJudith Sánchez-RodríguezBackground Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells. Results Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under specific conditions. Exposure to CV at 50 μgmL-1 induced a reduction of approximately 50% in cell viability, while a similar cytotoxic effect was observed when cell culture was exposed to F1 at 25 μgmL -1 or F2 at 50 μgmL-1. The cell culture exposure to F1 (10 μgmL-1) fraction combined with cisplatine (25 μM) provoked a decrease in MTT reduction until 65.57% while F2 (25 μgmL-1) fraction combined with cisplatin (10 μM) provoked a decrease in MTT reduction of 72.55%. Conclusions The F1 fraction had the greatest effect on the lung adenocarcinoma cell line compared with CV and F2. The combination of antineoplastic drugs and sea anemone toxins might allow a reduction of chemotherapeutic doses and thus mitigate side effects.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100308&lng=en&tlng=enCnidariaPharmacologyHuman lung cancer cellsCytotoxicity assayCisplatin efficacy |
| spellingShingle | Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells Journal of Venomous Animals and Toxins including Tropical Diseases Cnidaria Pharmacology Human lung cancer cells Cytotoxicity assay Cisplatin efficacy |
| title | Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
| title_full | Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
| title_fullStr | Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
| title_full_unstemmed | Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
| title_short | Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
| title_sort | toxins from the caribbean sea anemone bunodeopsis globulifera increase cisplatin induced cytotoxicity of lung adenocarcinoma cells |
| topic | Cnidaria Pharmacology Human lung cancer cells Cytotoxicity assay Cisplatin efficacy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100308&lng=en&tlng=en |
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