Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface
We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-08-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/11/8/8018/ |
| _version_ | 1818040866949824512 |
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| author | Shiping Song Sanjay Mathur Min Zhang Chunhai Fan Hao Shen Lanying Li Li Xu Gang Liu Qin Xu Ruojun Lao |
| author_facet | Shiping Song Sanjay Mathur Min Zhang Chunhai Fan Hao Shen Lanying Li Li Xu Gang Liu Qin Xu Ruojun Lao |
| author_sort | Shiping Song |
| collection | DOAJ |
| description | We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied. |
| first_indexed | 2024-12-10T08:21:20Z |
| format | Article |
| id | doaj.art-7ad22e9f14ce496c98f605895e853485 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T08:21:20Z |
| publishDate | 2011-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7ad22e9f14ce496c98f605895e8534852022-12-22T01:56:20ZengMDPI AGSensors1424-82202011-08-011188018802710.3390/s110808018Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling SurfaceShiping SongSanjay MathurMin ZhangChunhai FanHao ShenLanying LiLi XuGang LiuQin XuRuojun LaoWe report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.http://www.mdpi.com/1424-8220/11/8/8018/electrochemical biosensorsingle-nucleotide polymorphism (SNP)nonfouling electrode surfaceEscherichia coli |
| spellingShingle | Shiping Song Sanjay Mathur Min Zhang Chunhai Fan Hao Shen Lanying Li Li Xu Gang Liu Qin Xu Ruojun Lao Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface Sensors electrochemical biosensor single-nucleotide polymorphism (SNP) nonfouling electrode surface Escherichia coli |
| title | Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface |
| title_full | Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface |
| title_fullStr | Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface |
| title_full_unstemmed | Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface |
| title_short | Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface |
| title_sort | detection of single nucleotide polymorphism on uida gene of escherichia coli by a multiplexed electrochemical dna biosensor with oligonucleotide incorporated nonfouling surface |
| topic | electrochemical biosensor single-nucleotide polymorphism (SNP) nonfouling electrode surface Escherichia coli |
| url | http://www.mdpi.com/1424-8220/11/8/8018/ |
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