Assessment of trec and krec levels in COVID-19 patients with varying disease severity
According to the WHO data, the number of infected people exceeded 765.2 million people during the COVID-19 pandemic. The severity of patient’s condition is determined by immune system hyperactivation. Activation of T- and B-lymphocyte subsets plays a prominent role in the control of infectious proce...
| Príomhchruthaitheoirí: | , , , , , , , , , , |
|---|---|
| Formáid: | Alt |
| Teanga: | Russian |
| Foilsithe / Cruthaithe: |
Sankt-Peterburg : NIIÈM imeni Pastera
2023-11-01
|
| Sraith: | Инфекция и иммунитет |
| Ábhair: | |
| Rochtain ar líne: | https://iimmun.ru/iimm/article/viewFile/16937/1813 |
| _version_ | 1827582493529735168 |
|---|---|
| author | M. A. Saitgalina Yulia V. Ostankova Natalia A. Arsentieva Zoya R. Korobova Natalia E. Liubimova Victor A. Kashchenko Alexandr N. Kulikov Dmitry E. Pevtsov Oksana V. Stanevich Ekaterina I. Chernykh Areg A. Totolian |
| author_facet | M. A. Saitgalina Yulia V. Ostankova Natalia A. Arsentieva Zoya R. Korobova Natalia E. Liubimova Victor A. Kashchenko Alexandr N. Kulikov Dmitry E. Pevtsov Oksana V. Stanevich Ekaterina I. Chernykh Areg A. Totolian |
| author_sort | M. A. Saitgalina |
| collection | DOAJ |
| description | According to the WHO data, the number of infected people exceeded 765.2 million people during the COVID-19 pandemic. The severity of patient’s condition is determined by immune system hyperactivation. Activation of T- and B-lymphocyte subsets plays a prominent role in the control of infectious process. A content of small circular DNA molecules — T-cell receptor excision circles (TREC — T-cell receptor excision circles) and B-cell (“kappa”) excision rings (KREC — Kappa-deleting recombination excision circles) — in the peripheral blood can be used as a marker of the functionally active T and B cells maturation. The purpose of this work is to quantify peripheral blood TREC and KREC level in patients with the new coronavirus infection COVID-19 of varying severity.Materials and methods.The material consisted of 1028 blood samples from patients with a confirmed diagnosis of COVID-19, as well as 717 blood samples from apparently healthy volunteers. The content of TREC and KREC DNA fragments in the total DNA fraction was assessed by quantitative Real-time PCR using the “TREC/KREC-AMP PS” test system (St. Petersburg Pasteur Institute, Russia). Blood cell phenotyping was carried out using flow cytometry.Results.TREC/KREC levels were significantly reduced in COVID-19 patients (p 0.0001 at 95% CI). A significant direct correlation was established between the levels of peripheral blood TREC molecules and level of CD45+CD3+CD19–T cells (r = 0.59, p 0.0001), as well as between KREC level and count of CD45+CD3–CD19+B cells (r = 0.66, p 0.0001). The level of TREC molecules in patients with severe vs. moderate infection was significantly reduced in patients aged 30–39 years old (p = 0.0404) and 40–49 years old (p = 0.0356). The negative correlation between severity of COVID-19 clinical manifestations and TREC level in the blood of patients in 30–49 year age group indicates about an opportunity of using this analyte as a diagnostic and prognostic laboratory marker of patient’s condition. A simple PCR analysis algorithm makes it relevant to use the described method for assessing a state of immunity in coronavirus patients in the context of systemic negative impact of the SARS-CoV-2 virus on human organism. |
| first_indexed | 2024-03-08T22:52:34Z |
| format | Article |
| id | doaj.art-7ad243a01b444d3bbb51a66614b46726 |
| institution | Directory Open Access Journal |
| issn | 2220-7619 2313-7398 |
| language | Russian |
| last_indexed | 2024-03-08T22:52:34Z |
| publishDate | 2023-11-01 |
| publisher | Sankt-Peterburg : NIIÈM imeni Pastera |
| record_format | Article |
| series | Инфекция и иммунитет |
| spelling | doaj.art-7ad243a01b444d3bbb51a66614b467262023-12-16T10:50:05ZrusSankt-Peterburg : NIIÈM imeni PasteraИнфекция и иммунитет2220-76192313-73982023-11-0113587388410.15789/2220-7619-AOT-169371330Assessment of trec and krec levels in COVID-19 patients with varying disease severityM. A. Saitgalina0https://orcid.org/0000-0002-7603-3269Yulia V. Ostankova1https://orcid.org/0000-0003-2270-8897Natalia A. Arsentieva2https://orcid.org/0000-0003-2490-308XZoya R. Korobova3https://orcid.org/0000-0003-0535-5014Natalia E. Liubimova4https://orcid.org/0009-0003-7092-6773Victor A. Kashchenko5https://orcid.org/0000-0001-8822-9165Alexandr N. Kulikov6Dmitry E. Pevtsov7Oksana V. Stanevich8https://orcid.org/0000-0002-6894-6121Ekaterina I. Chernykh9Areg A. Totolian10https://orcid.org/0000-0003-4571-8799St. Petersburg Pasteur InstituteSt. Petersburg Pasteur InstituteSt. Petersburg Pasteur InstituteSt. Petersburg Pasteur InstituteSt. Petersburg Pasteur InstituteNorth-Western District Scientific and Clinical Center named after L.G. Sokolov Federal Medical and Biological AgencyI.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian FederationI.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian FederationI.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian FederationNorth-Western District Scientific and Clinical Center named after L.G. Sokolov Federal Medical and Biological AgencySt. Petersburg Pasteur InstituteAccording to the WHO data, the number of infected people exceeded 765.2 million people during the COVID-19 pandemic. The severity of patient’s condition is determined by immune system hyperactivation. Activation of T- and B-lymphocyte subsets plays a prominent role in the control of infectious process. A content of small circular DNA molecules — T-cell receptor excision circles (TREC — T-cell receptor excision circles) and B-cell (“kappa”) excision rings (KREC — Kappa-deleting recombination excision circles) — in the peripheral blood can be used as a marker of the functionally active T and B cells maturation. The purpose of this work is to quantify peripheral blood TREC and KREC level in patients with the new coronavirus infection COVID-19 of varying severity.Materials and methods.The material consisted of 1028 blood samples from patients with a confirmed diagnosis of COVID-19, as well as 717 blood samples from apparently healthy volunteers. The content of TREC and KREC DNA fragments in the total DNA fraction was assessed by quantitative Real-time PCR using the “TREC/KREC-AMP PS” test system (St. Petersburg Pasteur Institute, Russia). Blood cell phenotyping was carried out using flow cytometry.Results.TREC/KREC levels were significantly reduced in COVID-19 patients (p 0.0001 at 95% CI). A significant direct correlation was established between the levels of peripheral blood TREC molecules and level of CD45+CD3+CD19–T cells (r = 0.59, p 0.0001), as well as between KREC level and count of CD45+CD3–CD19+B cells (r = 0.66, p 0.0001). The level of TREC molecules in patients with severe vs. moderate infection was significantly reduced in patients aged 30–39 years old (p = 0.0404) and 40–49 years old (p = 0.0356). The negative correlation between severity of COVID-19 clinical manifestations and TREC level in the blood of patients in 30–49 year age group indicates about an opportunity of using this analyte as a diagnostic and prognostic laboratory marker of patient’s condition. A simple PCR analysis algorithm makes it relevant to use the described method for assessing a state of immunity in coronavirus patients in the context of systemic negative impact of the SARS-CoV-2 virus on human organism.https://iimmun.ru/iimm/article/viewFile/16937/1813treckreccovid-19sars-cov-2immune statuslaboratory markers |
| spellingShingle | M. A. Saitgalina Yulia V. Ostankova Natalia A. Arsentieva Zoya R. Korobova Natalia E. Liubimova Victor A. Kashchenko Alexandr N. Kulikov Dmitry E. Pevtsov Oksana V. Stanevich Ekaterina I. Chernykh Areg A. Totolian Assessment of trec and krec levels in COVID-19 patients with varying disease severity Инфекция и иммунитет trec krec covid-19 sars-cov-2 immune status laboratory markers |
| title | Assessment of trec and krec levels in COVID-19 patients with varying disease severity |
| title_full | Assessment of trec and krec levels in COVID-19 patients with varying disease severity |
| title_fullStr | Assessment of trec and krec levels in COVID-19 patients with varying disease severity |
| title_full_unstemmed | Assessment of trec and krec levels in COVID-19 patients with varying disease severity |
| title_short | Assessment of trec and krec levels in COVID-19 patients with varying disease severity |
| title_sort | assessment of trec and krec levels in covid 19 patients with varying disease severity |
| topic | trec krec covid-19 sars-cov-2 immune status laboratory markers |
| url | https://iimmun.ru/iimm/article/viewFile/16937/1813 |
| work_keys_str_mv | AT masaitgalina assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT yuliavostankova assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT nataliaaarsentieva assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT zoyarkorobova assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT nataliaeliubimova assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT victorakashchenko assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT alexandrnkulikov assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT dmitryepevtsov assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT oksanavstanevich assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT ekaterinaichernykh assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity AT aregatotolian assessmentoftrecandkreclevelsincovid19patientswithvaryingdiseaseseverity |