Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings
In light of expected climate change, it is important to seek nature-based solutions that can contribute to the protection of our planet as well as to help overcome the emerging adverse changes. In an agricultural context, increasing plant resistance to abiotic stress seems to be crucial. Therefore,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/6/843 |
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| author | Katarzyna Pacyga Paweł Pacyga Aleksandra Boba Bartosz Kozak Łukasz Wolko Yelyzaveta Kochneva Izabela Michalak |
| author_facet | Katarzyna Pacyga Paweł Pacyga Aleksandra Boba Bartosz Kozak Łukasz Wolko Yelyzaveta Kochneva Izabela Michalak |
| author_sort | Katarzyna Pacyga |
| collection | DOAJ |
| description | In light of expected climate change, it is important to seek nature-based solutions that can contribute to the protection of our planet as well as to help overcome the emerging adverse changes. In an agricultural context, increasing plant resistance to abiotic stress seems to be crucial. Therefore, the scope of the presented research was focused on the application of botanical extracts that exerted positive effects on model plants growing under controlled laboratory conditions, as well as plants subjected to sorbitol-induced osmotic stress. Foliar spraying increased the length and fresh mass of the shoots (e.g., extracts from <i>Taraxacum officinale</i>, <i>Trifolium pratense</i>, and <i>Pisum sativum</i>) and the roots (e.g., <i>Solidago gigantea</i>, <i>Hypericum perforatum</i>, and <i>Pisum sativum</i>) of cabbage seedlings grown under stressful conditions, as well as their content of photosynthetic pigments (<i>Pisum sativum</i>, <i>Lens culinaris</i>, and <i>Hypericum perforatum</i>) along with total phenolic compounds (<i>Hypericum perforatum</i>, <i>Taraxacum officinale</i>, and <i>Urtica dioica</i>). The antioxidant activity of the shoots measured with the use of DDPH (<i>Pisum sativum</i>, <i>Taraxacum officinale</i>, <i>Urtica dioica</i>, and <i>Hypericum perforatum</i>), ABTS (<i>Trifolium pratense</i>, <i>Symphytum officinale</i>, <i>Valeriana officinalis</i>, <i>Pisum sativum</i>, and <i>Lens culinaris</i>), and FRAP (<i>Symphytum officinale</i>, <i>Valeriana officinalis</i>, <i>Urtica dioica</i>, <i>Hypericum perforatum</i>, and <i>Taraxacum officinale</i>) assays was also enhanced in plants exposed to osmotic stress. Based on these findings, the most promising formulation based on <i>Symphytum officinale</i> was selected and subjected to transcriptomic analysis. The modification of the expression of the following genes was noted: <i>Bol029651</i> (glutathione S-transferase), <i>Bol027348</i> (chlorophyll A-B binding protein), <i>Bol015841</i> (S-adenosylmethionine-dependent methyltransferases), <i>Bol009860</i> (chlorophyll A-B binding protein), <i>Bol022819</i> (GDSL lipase/esterase), <i>Bol036512</i> (heat shock protein 70 family), <i>Bol005916</i> (DnaJ Chaperone), <i>Bol028754</i> (pre-mRNA splicing Prp18-interacting factor), <i>Bol009568</i> (heat shock protein Hsp90 family), <i>Bol039362</i> (gibberellin regulated protein), <i>Bol007693</i> (B-box-type zinc finger), <i>Bol034610</i> (RmlC-like cupin domain superfamily), <i>Bol019811</i> (myb_SHAQKYF: myb-like DNA-binding domain, SHAQKYF class), <i>Bol028965</i> (DA1-like Protein). Gene Ontology functional analysis indicated that the application of the extract led to a decrease in the expression of many genes related to the response to stress and photosynthetic systems, which may confirm a reduction in the level of oxidative stress in plants treated with biostimulants. The conducted studies showed that the use of innovative plant-based products exerted positive effects on crops and can be used to supplement current cultivation practices. |
| first_indexed | 2024-04-24T17:53:53Z |
| format | Article |
| id | doaj.art-7ad2f247dc164b9d8adb7e12b48f4238 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T17:53:53Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-7ad2f247dc164b9d8adb7e12b48f42382024-03-27T14:00:49ZengMDPI AGPlants2223-77472024-03-0113684310.3390/plants13060843Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage SeedlingsKatarzyna Pacyga0Paweł Pacyga1Aleksandra Boba2Bartosz Kozak3Łukasz Wolko4Yelyzaveta Kochneva5Izabela Michalak6Department of Environment Hygiene and Animal Welfare, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandDepartment of Thermodynamics and Renewable Energy Sources, Faculty of Mechanical and Power Engineering, Wrocław University of Science and Technology, 50-370 Wrocław, PolandDepartment of Genetic Biochemistry, Faculty of Biotechnology, University of Wrocław, 51-148 Wrocław, PolandDepartment of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wrocław University of Environmental and Life Sciences, 50-363 Wrocław, PolandDepartment of Biochemistry and Biotechnology, Faculty of Agriculture, Horticulture and Bioengineering, Poznan University of Life Sciences, 60-632 Poznań, PolandDepartment of Genetic Biochemistry, Faculty of Biotechnology, University of Wrocław, 51-148 Wrocław, PolandDepartment of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, 50-372 Wrocław, PolandIn light of expected climate change, it is important to seek nature-based solutions that can contribute to the protection of our planet as well as to help overcome the emerging adverse changes. In an agricultural context, increasing plant resistance to abiotic stress seems to be crucial. Therefore, the scope of the presented research was focused on the application of botanical extracts that exerted positive effects on model plants growing under controlled laboratory conditions, as well as plants subjected to sorbitol-induced osmotic stress. Foliar spraying increased the length and fresh mass of the shoots (e.g., extracts from <i>Taraxacum officinale</i>, <i>Trifolium pratense</i>, and <i>Pisum sativum</i>) and the roots (e.g., <i>Solidago gigantea</i>, <i>Hypericum perforatum</i>, and <i>Pisum sativum</i>) of cabbage seedlings grown under stressful conditions, as well as their content of photosynthetic pigments (<i>Pisum sativum</i>, <i>Lens culinaris</i>, and <i>Hypericum perforatum</i>) along with total phenolic compounds (<i>Hypericum perforatum</i>, <i>Taraxacum officinale</i>, and <i>Urtica dioica</i>). The antioxidant activity of the shoots measured with the use of DDPH (<i>Pisum sativum</i>, <i>Taraxacum officinale</i>, <i>Urtica dioica</i>, and <i>Hypericum perforatum</i>), ABTS (<i>Trifolium pratense</i>, <i>Symphytum officinale</i>, <i>Valeriana officinalis</i>, <i>Pisum sativum</i>, and <i>Lens culinaris</i>), and FRAP (<i>Symphytum officinale</i>, <i>Valeriana officinalis</i>, <i>Urtica dioica</i>, <i>Hypericum perforatum</i>, and <i>Taraxacum officinale</i>) assays was also enhanced in plants exposed to osmotic stress. Based on these findings, the most promising formulation based on <i>Symphytum officinale</i> was selected and subjected to transcriptomic analysis. The modification of the expression of the following genes was noted: <i>Bol029651</i> (glutathione S-transferase), <i>Bol027348</i> (chlorophyll A-B binding protein), <i>Bol015841</i> (S-adenosylmethionine-dependent methyltransferases), <i>Bol009860</i> (chlorophyll A-B binding protein), <i>Bol022819</i> (GDSL lipase/esterase), <i>Bol036512</i> (heat shock protein 70 family), <i>Bol005916</i> (DnaJ Chaperone), <i>Bol028754</i> (pre-mRNA splicing Prp18-interacting factor), <i>Bol009568</i> (heat shock protein Hsp90 family), <i>Bol039362</i> (gibberellin regulated protein), <i>Bol007693</i> (B-box-type zinc finger), <i>Bol034610</i> (RmlC-like cupin domain superfamily), <i>Bol019811</i> (myb_SHAQKYF: myb-like DNA-binding domain, SHAQKYF class), <i>Bol028965</i> (DA1-like Protein). Gene Ontology functional analysis indicated that the application of the extract led to a decrease in the expression of many genes related to the response to stress and photosynthetic systems, which may confirm a reduction in the level of oxidative stress in plants treated with biostimulants. The conducted studies showed that the use of innovative plant-based products exerted positive effects on crops and can be used to supplement current cultivation practices.https://www.mdpi.com/2223-7747/13/6/843plant-based extractsprosperous biostimulantsPetri dish testsfoliar spraysorbitol-induced stresschemical analysis |
| spellingShingle | Katarzyna Pacyga Paweł Pacyga Aleksandra Boba Bartosz Kozak Łukasz Wolko Yelyzaveta Kochneva Izabela Michalak Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings Plants plant-based extracts prosperous biostimulants Petri dish tests foliar spray sorbitol-induced stress chemical analysis |
| title | Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings |
| title_full | Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings |
| title_fullStr | Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings |
| title_full_unstemmed | Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings |
| title_short | Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings |
| title_sort | potential of plant based extracts to alleviate sorbitol induced osmotic stress in cabbage seedlings |
| topic | plant-based extracts prosperous biostimulants Petri dish tests foliar spray sorbitol-induced stress chemical analysis |
| url | https://www.mdpi.com/2223-7747/13/6/843 |
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