Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients
IntroductionIntestinal colonization by Multi-Drug Resistant Organisms (MDROs) can pose a threat on the health of critically ill patients. The extent of colonization by these organisms is related to previous antibiotic treatments and their ability to cause infections among adult patients. The aim of...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1180714/full |
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| author | Elias Dahdouh Emilio Cendejas-Bueno Emilio Cendejas-Bueno Guillermo Ruiz-Carrascoso Guillermo Ruiz-Carrascoso Cristina Schüffelmann Fernando Lázaro-Perona Mercedes Castro-Martínez Francisco Moreno-Ramos Luis Escosa-García Luis Escosa-García Marina Alguacil-Guillén Jesús Mingorance Jesús Mingorance |
| author_facet | Elias Dahdouh Emilio Cendejas-Bueno Emilio Cendejas-Bueno Guillermo Ruiz-Carrascoso Guillermo Ruiz-Carrascoso Cristina Schüffelmann Fernando Lázaro-Perona Mercedes Castro-Martínez Francisco Moreno-Ramos Luis Escosa-García Luis Escosa-García Marina Alguacil-Guillén Jesús Mingorance Jesús Mingorance |
| author_sort | Elias Dahdouh |
| collection | DOAJ |
| description | IntroductionIntestinal colonization by Multi-Drug Resistant Organisms (MDROs) can pose a threat on the health of critically ill patients. The extent of colonization by these organisms is related to previous antibiotic treatments and their ability to cause infections among adult patients. The aim of this study is to determine the relationship between the intestinal Relative Loads (RLs) of selected antibiotic resistance genes, antibiotic consumption and extra-intestinal spread among critically ill pediatric patients.MethodsRLs of blaCTX-M-1-Family, blaOXA-1, blaOXA-48 and blaVIM were determined in 382 rectal swabs obtained from 90 pediatric critically ill patients using qPCRs. The RLs were compared to the patients’ demographics, antibiotic consumption, and detection of MDROs from extra-intestinal sites. 16SrDNA metagenomic sequencing was performed for 40 samples and clonality analyses were done for representative isolates.Results and discussion76 (74.45%) patients from which 340 (89.01%) rectal swabs were collected had at least one swab that was positive for one of the tested genes. Routine cultures did not identify carbapenemases in 32 (45.1%) and 78 (58.2%) swabs that were positive by PCR for blaOXA-48 and blaVIM, respectively. RLs of above 6.5% were associated with extra-intestinal spread of blaOXA-48-harboring MDROs. Consumption of carbapenems, non-carbapenem β-lactams, and glycopeptides were statistically associated with testing negative for blaCTX-M-1-Family and blaOXA-1 while the consumption of trimethoprim/sulfamethoxazole and aminoglycosides was associated with testing negative for blaOXA-48 (P<0.05). In conclusion, targeted qPCRs can be used to determine the extent of intestinal dominance by antibiotic resistant opportunistic pathogens and their potential to cause extra-intestinal infections among a critically ill pediatric population. |
| first_indexed | 2024-04-09T14:44:36Z |
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| language | English |
| last_indexed | 2024-04-09T14:44:36Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-7ad960329d6f41379179ccd3ceae47fc2023-05-02T20:30:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-05-011310.3389/fcimb.2023.11807141180714Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patientsElias Dahdouh0Emilio Cendejas-Bueno1Emilio Cendejas-Bueno2Guillermo Ruiz-Carrascoso3Guillermo Ruiz-Carrascoso4Cristina Schüffelmann5Fernando Lázaro-Perona6Mercedes Castro-Martínez7Francisco Moreno-Ramos8Luis Escosa-García9Luis Escosa-García10Marina Alguacil-Guillén11Jesús Mingorance12Jesús Mingorance13Clinical Microbiology and Parasitology Department, Hospital Universitario La Paz, Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, SpainClinical Microbiology and Parasitology Department, Hospital Universitario La Paz, Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, SpainClinical Microbiology and Parasitology Department, Hospital Universitario La Paz, Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, SpainPediatric Intensive Care Unit, Hospital Universitario La Paz, Madrid, SpainClinical Microbiology and Parasitology Department, Hospital Universitario La Paz, Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, SpainPreventive Medicine Department, Hospital Universitario La Paz, Madrid, SpainDepartment of Pharmacy, Hospital Universitario La Paz, Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, SpainPediatric Tropical and Infectious Diseases Department, Hospital Universitario La Paz, Madrid, SpainClinical Microbiology and Parasitology Department, Hospital Universitario La Paz, Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, SpainClinical Microbiology and Parasitology Department, Hospital Universitario La Paz, Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, SpainIntroductionIntestinal colonization by Multi-Drug Resistant Organisms (MDROs) can pose a threat on the health of critically ill patients. The extent of colonization by these organisms is related to previous antibiotic treatments and their ability to cause infections among adult patients. The aim of this study is to determine the relationship between the intestinal Relative Loads (RLs) of selected antibiotic resistance genes, antibiotic consumption and extra-intestinal spread among critically ill pediatric patients.MethodsRLs of blaCTX-M-1-Family, blaOXA-1, blaOXA-48 and blaVIM were determined in 382 rectal swabs obtained from 90 pediatric critically ill patients using qPCRs. The RLs were compared to the patients’ demographics, antibiotic consumption, and detection of MDROs from extra-intestinal sites. 16SrDNA metagenomic sequencing was performed for 40 samples and clonality analyses were done for representative isolates.Results and discussion76 (74.45%) patients from which 340 (89.01%) rectal swabs were collected had at least one swab that was positive for one of the tested genes. Routine cultures did not identify carbapenemases in 32 (45.1%) and 78 (58.2%) swabs that were positive by PCR for blaOXA-48 and blaVIM, respectively. RLs of above 6.5% were associated with extra-intestinal spread of blaOXA-48-harboring MDROs. Consumption of carbapenems, non-carbapenem β-lactams, and glycopeptides were statistically associated with testing negative for blaCTX-M-1-Family and blaOXA-1 while the consumption of trimethoprim/sulfamethoxazole and aminoglycosides was associated with testing negative for blaOXA-48 (P<0.05). In conclusion, targeted qPCRs can be used to determine the extent of intestinal dominance by antibiotic resistant opportunistic pathogens and their potential to cause extra-intestinal infections among a critically ill pediatric population.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1180714/fullintestinal dominancepediatric patientsβ-lactamase genesrelative intestinal loadqPCRantibiotic consumption |
| spellingShingle | Elias Dahdouh Emilio Cendejas-Bueno Emilio Cendejas-Bueno Guillermo Ruiz-Carrascoso Guillermo Ruiz-Carrascoso Cristina Schüffelmann Fernando Lázaro-Perona Mercedes Castro-Martínez Francisco Moreno-Ramos Luis Escosa-García Luis Escosa-García Marina Alguacil-Guillén Jesús Mingorance Jesús Mingorance Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients Frontiers in Cellular and Infection Microbiology intestinal dominance pediatric patients β-lactamase genes relative intestinal load qPCR antibiotic consumption |
| title | Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients |
| title_full | Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients |
| title_fullStr | Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients |
| title_full_unstemmed | Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients |
| title_short | Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients |
| title_sort | intestinal loads of extended spectrum beta lactamase and carbapenemase genes in critically ill pediatric patients |
| topic | intestinal dominance pediatric patients β-lactamase genes relative intestinal load qPCR antibiotic consumption |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1180714/full |
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