The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry
ObjectiveTo establish a high-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the detection of 6 quinolones residues in milk tofu.MethodsThe milk tofu sample was extracted with 0.1 mmol/L EDTA-Mcllvaine buffer with 0.1 mL each of ferrocyanide and zinc acetate， purif...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The Editorial Office of Chinese Journal of Food Hygiene
2023-03-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202303004 |
| Summary: | ObjectiveTo establish a high-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the detection of 6 quinolones residues in milk tofu.MethodsThe milk tofu sample was extracted with 0.1 mmol/L EDTA-Mcllvaine buffer with 0.1 mL each of ferrocyanide and zinc acetate， purified by HLB Pro solid phase extraction column， and separated by Shim-pack FC-ODS （150 mm×20 mm， 3 μm）， gradient eluted by 10 mmol/L ammonium acetate （containing 0.1% formic acid）-methanol acetonitrile （40：60， V/V）， and detected in full scan （full MS） mode.ResultsThe results showed that the 6 quinolones had good linearity in the concentration range of 10-100 ng/mL， the correlation coefficient （R2） was all above 0.995， the limit of detection （LOD） was 0.5-1.5 μg/kg， the limit of quantification （LOQ） was 2-4 μg/kg， the recovery rate of spiked sample was 82.3%-101.6%， and the relative standard deviation was less than 10%.ConclusionThis method is highly sensitive， rapid and accurate， and suitable for rapid screening， quantitative and qualitative analysis of 6 quinolones in milk tofu. |
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| ISSN: | 1004-8456 |