The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry
ObjectiveTo establish a high-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the detection of 6 quinolones residues in milk tofu.MethodsThe milk tofu sample was extracted with 0.1 mmol/L EDTA-Mcllvaine buffer with 0.1 mL each of ferrocyanide and zinc acetate， purif...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2023-03-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202303004 |
| _version_ | 1797821012921810944 |
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| author | WANG Xueqi ZHANG Xuefu YANG Xiaolei SONG Lihua YANG Yuhao LI Xiangyang |
| author_facet | WANG Xueqi ZHANG Xuefu YANG Xiaolei SONG Lihua YANG Yuhao LI Xiangyang |
| author_sort | WANG Xueqi |
| collection | DOAJ |
| description | ObjectiveTo establish a high-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the detection of 6 quinolones residues in milk tofu.MethodsThe milk tofu sample was extracted with 0.1 mmol/L EDTA-Mcllvaine buffer with 0.1 mL each of ferrocyanide and zinc acetate， purified by HLB Pro solid phase extraction column， and separated by Shim-pack FC-ODS （150 mm×20 mm， 3 μm）， gradient eluted by 10 mmol/L ammonium acetate （containing 0.1% formic acid）-methanol acetonitrile （40：60， V/V）， and detected in full scan （full MS） mode.ResultsThe results showed that the 6 quinolones had good linearity in the concentration range of 10-100 ng/mL， the correlation coefficient （R2） was all above 0.995， the limit of detection （LOD） was 0.5-1.5 μg/kg， the limit of quantification （LOQ） was 2-4 μg/kg， the recovery rate of spiked sample was 82.3%-101.6%， and the relative standard deviation was less than 10%.ConclusionThis method is highly sensitive， rapid and accurate， and suitable for rapid screening， quantitative and qualitative analysis of 6 quinolones in milk tofu. |
| first_indexed | 2024-03-13T09:46:34Z |
| format | Article |
| id | doaj.art-7ada95f4a3844083880da984d8f277cf |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-13T09:46:34Z |
| publishDate | 2023-03-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-7ada95f4a3844083880da984d8f277cf2023-05-25T02:13:58ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-03-0135333934510.13590/j.cjfh.2023.03.0041004-8456（2023）03-0339-07The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometryWANG Xueqi0ZHANG Xuefu1YANG Xiaolei2SONG Lihua3YANG Yuhao4LI Xiangyang5College of Animal Science and Technology, Inner Mongolia Universityfor Nationalities, Inner Mongolia Tongliao 028000, ChinaAnalysis and Testing Center, Inner Mongolia University for Nationalities, Inner Mongolia Tongliao 028000, ChinaFood Room of Tongliao Food and Drug Inspection Institute, Inner Mongolia Tongliao 028000, ChinaAffiliated Hospital of Inner Mongolia University for Nationalities, Inner Mongolia Tongliao 028000, ChinaCollege of Animal Science and Technology, Inner Mongolia Universityfor Nationalities, Inner Mongolia Tongliao 028000, ChinaCollege of Animal Science and Technology, Inner Mongolia Universityfor Nationalities, Inner Mongolia Tongliao 028000, ChinaObjectiveTo establish a high-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the detection of 6 quinolones residues in milk tofu.MethodsThe milk tofu sample was extracted with 0.1 mmol/L EDTA-Mcllvaine buffer with 0.1 mL each of ferrocyanide and zinc acetate， purified by HLB Pro solid phase extraction column， and separated by Shim-pack FC-ODS （150 mm×20 mm， 3 μm）， gradient eluted by 10 mmol/L ammonium acetate （containing 0.1% formic acid）-methanol acetonitrile （40：60， V/V）， and detected in full scan （full MS） mode.ResultsThe results showed that the 6 quinolones had good linearity in the concentration range of 10-100 ng/mL， the correlation coefficient （R2） was all above 0.995， the limit of detection （LOD） was 0.5-1.5 μg/kg， the limit of quantification （LOQ） was 2-4 μg/kg， the recovery rate of spiked sample was 82.3%-101.6%， and the relative standard deviation was less than 10%.ConclusionThis method is highly sensitive， rapid and accurate， and suitable for rapid screening， quantitative and qualitative analysis of 6 quinolones in milk tofu.http://www.zgspws.com/zgspwszz/article/abstract/202303004uplc-ms/msquinolonemilk tofudrug residue |
| spellingShingle | WANG Xueqi ZHANG Xuefu YANG Xiaolei SONG Lihua YANG Yuhao LI Xiangyang The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry Zhongguo shipin weisheng zazhi uplc-ms/ms quinolone milk tofu drug residue |
| title | The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry |
| title_full | The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry |
| title_fullStr | The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry |
| title_full_unstemmed | The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry |
| title_short | The determination of six quinolone drug residues in milk tofu by high-performance liquid chromatography-tandem mass spectrometry |
| title_sort | determination of six quinolone drug residues in milk tofu by high performance liquid chromatography tandem mass spectrometry |
| topic | uplc-ms/ms quinolone milk tofu drug residue |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202303004 |
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