Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery
Backgrounds: SARS-CoV-2 infection results in a broad spectrum of clinical outcomes, ranging from asymptomatic to severe symptoms and death. Most COVID-19 pathogenesis is associated with hyperinflammatory conditions driven primarily by myeloid cell lineages. The long-term effects of SARS-CoV-2 infect...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118222001529 |
| _version_ | 1811183602723979264 |
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| author | Vichaya Ruenjaiman Pimpayao Sodsai Patipark Kueanjinda Worawan Bunrasmee Siriwan Klinchanhom Rangsima Reantragoon Chavit Tunvirachaisakul Kasama Manothummetha Nuthchaya Mejun Kaewkwan Liengswangwong Pattama Torvorapanit Leilani Paitoonpong Opass Putcharoen Tanapat Palaga Nattiya Hirankarn Abhichaya Tungwongkitsiri Chanya Mittrakulkij Farsai Chiewbangyang Janista Kaewsrihawong Jirayu Sanpakit Kanokphet Kulkiatprasert Khemmachat Munkong Nanthida Keawthawon Natchanon Wattanakul Natdanai Limchanachon Natthapat Roopsuwankun Natthasini Chaosuwannakij Pasin Larpanekanan Pawit Pitakkitnukun Pongpon Homswad Samapitch Ratanapraisorn Sarunyapong Atchariyapakorn Sasathamon Vongphanich Sirapat Jessadapornchai Teton Avihingsanon Thanatorn Piyasathapornpong |
| author_facet | Vichaya Ruenjaiman Pimpayao Sodsai Patipark Kueanjinda Worawan Bunrasmee Siriwan Klinchanhom Rangsima Reantragoon Chavit Tunvirachaisakul Kasama Manothummetha Nuthchaya Mejun Kaewkwan Liengswangwong Pattama Torvorapanit Leilani Paitoonpong Opass Putcharoen Tanapat Palaga Nattiya Hirankarn Abhichaya Tungwongkitsiri Chanya Mittrakulkij Farsai Chiewbangyang Janista Kaewsrihawong Jirayu Sanpakit Kanokphet Kulkiatprasert Khemmachat Munkong Nanthida Keawthawon Natchanon Wattanakul Natdanai Limchanachon Natthapat Roopsuwankun Natthasini Chaosuwannakij Pasin Larpanekanan Pawit Pitakkitnukun Pongpon Homswad Samapitch Ratanapraisorn Sarunyapong Atchariyapakorn Sasathamon Vongphanich Sirapat Jessadapornchai Teton Avihingsanon Thanatorn Piyasathapornpong |
| author_sort | Vichaya Ruenjaiman |
| collection | DOAJ |
| description | Backgrounds: SARS-CoV-2 infection results in a broad spectrum of clinical outcomes, ranging from asymptomatic to severe symptoms and death. Most COVID-19 pathogenesis is associated with hyperinflammatory conditions driven primarily by myeloid cell lineages. The long-term effects of SARS-CoV-2 infection post recovery include various symptoms. Methods: We performed a longitudinal study of the innate immune profiles 1 and 3 months after recovery in the Thai cohort by comparing patients with mild, moderate, and severe clinical symptoms using peripheral blood mononuclear cells (n = 62). Results: Significant increases in the frequencies of monocytes compared to controls and NK cells compared to mild and moderate patients were observed in severe patients 1–3 months post recovery. Increased polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) were observed in all recovered patients, even after 3 months. Increased IL-6 and TNFα levels in monocytes were observed 1 month after recovery in response to lipopolysaccharide (LPS) stimulation, while decreased CD86 and HLA-DR levels were observed regardless of stimulation. A multiplex analysis of serum cytokines performed at 1 month revealed that most innate cytokines, except for TNFα, IL4/IL-13 (Th2) and IFNγ (Th1), were elevated in recovered patients in a severity-dependent manner. Finally, the myelopoiesis cytokines G-CSF and GM-CSF were higher in all patient groups. Increased monocytes and IL-6- and TNFα-producing cells were significantly associated with long COVID-19 symptoms. Conclusions: These results reveal that COVID-19 infection influences the frequencies and functions of innate immune cells for up to 3 months after recovery, which may potentially lead to some of the long COVID symptoms. |
| first_indexed | 2024-04-11T09:49:45Z |
| format | Article |
| id | doaj.art-7adba5d9e9614abe9e0715b268753db9 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-04-11T09:49:45Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-7adba5d9e9614abe9e0715b268753db92022-12-22T04:30:51ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822022-12-015569931004Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recoveryVichaya Ruenjaiman0Pimpayao Sodsai1Patipark Kueanjinda2Worawan Bunrasmee3Siriwan Klinchanhom4Rangsima Reantragoon5Chavit Tunvirachaisakul6Kasama Manothummetha7Nuthchaya Mejun8Kaewkwan Liengswangwong9Pattama Torvorapanit10Leilani Paitoonpong11Opass Putcharoen12Tanapat Palaga13Nattiya Hirankarn14Abhichaya TungwongkitsiriChanya MittrakulkijFarsai ChiewbangyangJanista KaewsrihawongJirayu SanpakitKanokphet KulkiatprasertKhemmachat MunkongNanthida KeawthawonNatchanon WattanakulNatdanai LimchanachonNatthapat RoopsuwankunNatthasini ChaosuwannakijPasin LarpanekananPawit PitakkitnukunPongpon HomswadSamapitch RatanapraisornSarunyapong AtchariyapakornSasathamon VongphanichSirapat JessadapornchaiTeton AvihingsanonThanatorn PiyasathapornpongDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Psychiatry, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandThai Red Cross Emerging Infectious Diseases Clinical Centre, King Chulalongkorn Memorial Hospital, Bangkok, 10330, Thailand; Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandThai Red Cross Emerging Infectious Diseases Clinical Centre, King Chulalongkorn Memorial Hospital, Bangkok, 10330, Thailand; Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandThai Red Cross Emerging Infectious Diseases Clinical Centre, King Chulalongkorn Memorial Hospital, Bangkok, 10330, Thailand; Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandCenter of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, Thailand; Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Corresponding author. Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Chulalongkorn University, Bangkok, 10330, ThailandBackgrounds: SARS-CoV-2 infection results in a broad spectrum of clinical outcomes, ranging from asymptomatic to severe symptoms and death. Most COVID-19 pathogenesis is associated with hyperinflammatory conditions driven primarily by myeloid cell lineages. The long-term effects of SARS-CoV-2 infection post recovery include various symptoms. Methods: We performed a longitudinal study of the innate immune profiles 1 and 3 months after recovery in the Thai cohort by comparing patients with mild, moderate, and severe clinical symptoms using peripheral blood mononuclear cells (n = 62). Results: Significant increases in the frequencies of monocytes compared to controls and NK cells compared to mild and moderate patients were observed in severe patients 1–3 months post recovery. Increased polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) were observed in all recovered patients, even after 3 months. Increased IL-6 and TNFα levels in monocytes were observed 1 month after recovery in response to lipopolysaccharide (LPS) stimulation, while decreased CD86 and HLA-DR levels were observed regardless of stimulation. A multiplex analysis of serum cytokines performed at 1 month revealed that most innate cytokines, except for TNFα, IL4/IL-13 (Th2) and IFNγ (Th1), were elevated in recovered patients in a severity-dependent manner. Finally, the myelopoiesis cytokines G-CSF and GM-CSF were higher in all patient groups. Increased monocytes and IL-6- and TNFα-producing cells were significantly associated with long COVID-19 symptoms. Conclusions: These results reveal that COVID-19 infection influences the frequencies and functions of innate immune cells for up to 3 months after recovery, which may potentially lead to some of the long COVID symptoms.http://www.sciencedirect.com/science/article/pii/S1684118222001529COVID-19Innate immune cellsLipopolysaccharidePost recovery |
| spellingShingle | Vichaya Ruenjaiman Pimpayao Sodsai Patipark Kueanjinda Worawan Bunrasmee Siriwan Klinchanhom Rangsima Reantragoon Chavit Tunvirachaisakul Kasama Manothummetha Nuthchaya Mejun Kaewkwan Liengswangwong Pattama Torvorapanit Leilani Paitoonpong Opass Putcharoen Tanapat Palaga Nattiya Hirankarn Abhichaya Tungwongkitsiri Chanya Mittrakulkij Farsai Chiewbangyang Janista Kaewsrihawong Jirayu Sanpakit Kanokphet Kulkiatprasert Khemmachat Munkong Nanthida Keawthawon Natchanon Wattanakul Natdanai Limchanachon Natthapat Roopsuwankun Natthasini Chaosuwannakij Pasin Larpanekanan Pawit Pitakkitnukun Pongpon Homswad Samapitch Ratanapraisorn Sarunyapong Atchariyapakorn Sasathamon Vongphanich Sirapat Jessadapornchai Teton Avihingsanon Thanatorn Piyasathapornpong Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery Journal of Microbiology, Immunology and Infection COVID-19 Innate immune cells Lipopolysaccharide Post recovery |
| title | Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery |
| title_full | Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery |
| title_fullStr | Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery |
| title_full_unstemmed | Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery |
| title_short | Impact of SARS-CoV-2 infection on the profiles and responses of innate immune cells after recovery |
| title_sort | impact of sars cov 2 infection on the profiles and responses of innate immune cells after recovery |
| topic | COVID-19 Innate immune cells Lipopolysaccharide Post recovery |
| url | http://www.sciencedirect.com/science/article/pii/S1684118222001529 |
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