Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration
Processibility and biodegradability of conductive polymers are major concerns when they are applied to tissue regeneration. This study synthesizes dissolvable and conductive aniline trimer-based polyurethane copolymers (DCPU) and processes them into scaffolds by using electrospinning with different...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/14/4/185 |
| _version_ | 1797604881299668992 |
|---|---|
| author | Yinglong Zhang Jiajing Tang Wei Fang Qing Zhao Xiaoyu Lei Jinzheng Zhang Jieqiong Chen Yubao Li Yi Zuo |
| author_facet | Yinglong Zhang Jiajing Tang Wei Fang Qing Zhao Xiaoyu Lei Jinzheng Zhang Jieqiong Chen Yubao Li Yi Zuo |
| author_sort | Yinglong Zhang |
| collection | DOAJ |
| description | Processibility and biodegradability of conductive polymers are major concerns when they are applied to tissue regeneration. This study synthesizes dissolvable and conductive aniline trimer-based polyurethane copolymers (DCPU) and processes them into scaffolds by using electrospinning with different patterns (random, oriented, and latticed). The effects of topographic cue changes on electrical signal transmission and further regulation of cell behaviors concerning bone tissue are researched. Results show that DCPU fibrous scaffolds possessed good hydrophilicity, swelling capacity, elasticity, and fast biodegradability in enzymatic liquid. In addition, the conductivity and efficiency of electrical signal transmission can be tuned by changing the surface’s topological structure. Among them, oriented DCPU scaffolds (DCPU-O) showed the best conductivity with the lowest ionic resistance value. Furthermore, the viability and proliferation results of bone mesenchymal stem cells (BMSCs) demonstrate a significant increase on three DCPU scaffolds compared to AT-free scaffolds (DPU-R). Especially, DCPU-O scaffolds exhibit superior abilities to promote cell proliferation because of their unique surface topography and excellent electroactivity. Concurrently, the DCPU-O scaffolds can synergistically promote osteogenic differentiation in terms of osteogenic differentiation and gene expression levels when combined with electrical stimulation. Together, these results suggest a promising use of DCPU-O fibrous scaffolds in the application of tissue regeneration. |
| first_indexed | 2024-03-11T04:53:01Z |
| format | Article |
| id | doaj.art-7adcb166afdd40dc8baf6d6553ee40ab |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T04:53:01Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-7adcb166afdd40dc8baf6d6553ee40ab2023-11-17T19:52:56ZengMDPI AGJournal of Functional Biomaterials2079-49832023-03-0114418510.3390/jfb14040185Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue RegenerationYinglong Zhang0Jiajing Tang1Wei Fang2Qing Zhao3Xiaoyu Lei4Jinzheng Zhang5Jieqiong Chen6Yubao Li7Yi Zuo8Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaMOE Key Laboratory of Low-Grade Energy, Utilization Technologies and Systems, CQU-NUS Renewable, Energy Materials & Devices Joint Laboratory, School of Energy & Power Engineering, Chongqing University, Chongqing 400044, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaResearch Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, ChinaProcessibility and biodegradability of conductive polymers are major concerns when they are applied to tissue regeneration. This study synthesizes dissolvable and conductive aniline trimer-based polyurethane copolymers (DCPU) and processes them into scaffolds by using electrospinning with different patterns (random, oriented, and latticed). The effects of topographic cue changes on electrical signal transmission and further regulation of cell behaviors concerning bone tissue are researched. Results show that DCPU fibrous scaffolds possessed good hydrophilicity, swelling capacity, elasticity, and fast biodegradability in enzymatic liquid. In addition, the conductivity and efficiency of electrical signal transmission can be tuned by changing the surface’s topological structure. Among them, oriented DCPU scaffolds (DCPU-O) showed the best conductivity with the lowest ionic resistance value. Furthermore, the viability and proliferation results of bone mesenchymal stem cells (BMSCs) demonstrate a significant increase on three DCPU scaffolds compared to AT-free scaffolds (DPU-R). Especially, DCPU-O scaffolds exhibit superior abilities to promote cell proliferation because of their unique surface topography and excellent electroactivity. Concurrently, the DCPU-O scaffolds can synergistically promote osteogenic differentiation in terms of osteogenic differentiation and gene expression levels when combined with electrical stimulation. Together, these results suggest a promising use of DCPU-O fibrous scaffolds in the application of tissue regeneration.https://www.mdpi.com/2079-4983/14/4/185oligoanilineconductive polyurethanefibrous scaffoldselectrical and topographical cuestissue regeneration |
| spellingShingle | Yinglong Zhang Jiajing Tang Wei Fang Qing Zhao Xiaoyu Lei Jinzheng Zhang Jieqiong Chen Yubao Li Yi Zuo Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration Journal of Functional Biomaterials oligoaniline conductive polyurethane fibrous scaffolds electrical and topographical cues tissue regeneration |
| title | Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration |
| title_full | Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration |
| title_fullStr | Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration |
| title_full_unstemmed | Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration |
| title_short | Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration |
| title_sort | synergetic effect of electrical and topographical cues in aniline trimer based polyurethane fibrous scaffolds on tissue regeneration |
| topic | oligoaniline conductive polyurethane fibrous scaffolds electrical and topographical cues tissue regeneration |
| url | https://www.mdpi.com/2079-4983/14/4/185 |
| work_keys_str_mv | AT yinglongzhang synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT jiajingtang synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT weifang synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT qingzhao synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT xiaoyulei synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT jinzhengzhang synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT jieqiongchen synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT yubaoli synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration AT yizuo synergeticeffectofelectricalandtopographicalcuesinanilinetrimerbasedpolyurethanefibrousscaffoldsontissueregeneration |