Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells
Absorption spectra of red blood cell (RBC) suspensions are investigated in an osmolarity range in the medium from 200 mOsm to 900 mOsm. Three spectral parameters are used to characterize the process of swelling or shrinkage of RBC—the absorbance at 700 nm, the Soret peak height relative to the spect...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/13/7/589 |
| _version_ | 1827286904786124800 |
|---|---|
| author | Miroslav Karabaliev Bilyana Tacheva Boyana Paarvanova Radostina Georgieva |
| author_facet | Miroslav Karabaliev Bilyana Tacheva Boyana Paarvanova Radostina Georgieva |
| author_sort | Miroslav Karabaliev |
| collection | DOAJ |
| description | Absorption spectra of red blood cell (RBC) suspensions are investigated in an osmolarity range in the medium from 200 mOsm to 900 mOsm. Three spectral parameters are used to characterize the process of swelling or shrinkage of RBC—the absorbance at 700 nm, the Soret peak height relative to the spectrum background, and the Soret peak wavelength. We show that with an increase in the osmolarity, the absorbance at 700 nm increases and the Soret peak relative height decreases. These changes are related to the changes in the RBC volume and the resulting increase in the hemoglobin intracellular concentration and index of refraction. Confocal microscopy and flow cytometry measurements supported these conclusions. The maximum wavelength of the Soret peak increases with increasing osmolarity due to changes in the oxygenation state of hemoglobin. Using these spectrum parameters, the process of osmosis in RBCs can be followed in real time, but it can also be applied to various processes, leading to changes in the volume and shape of RBCs. Therefore, we conclude that UV–Vis absorption spectrophotometry offers a convenient, easily accessible, and cost-effective method to monitor changes in RBC, which can find applications in the field of drug discovery and diagnostics of RBC and hemoglobin disorders. |
| first_indexed | 2024-04-24T10:47:52Z |
| format | Article |
| id | doaj.art-7ae1109b41224b80acdf642d9662f905 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-04-24T10:47:52Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-7ae1109b41224b80acdf642d9662f9052024-04-12T13:16:28ZengMDPI AGCells2073-44092024-03-0113758910.3390/cells13070589Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood CellsMiroslav Karabaliev0Bilyana Tacheva1Boyana Paarvanova2Radostina Georgieva3Department of Physics and Biophysics, Faculty of Medicine, Trakia University, 11 Armeiska, 6000 Stara Zagora, BulgariaDepartment of Physics and Biophysics, Faculty of Medicine, Trakia University, 11 Armeiska, 6000 Stara Zagora, BulgariaDepartment of Physics and Biophysics, Faculty of Medicine, Trakia University, 11 Armeiska, 6000 Stara Zagora, BulgariaDepartment of Physics and Biophysics, Faculty of Medicine, Trakia University, 11 Armeiska, 6000 Stara Zagora, BulgariaAbsorption spectra of red blood cell (RBC) suspensions are investigated in an osmolarity range in the medium from 200 mOsm to 900 mOsm. Three spectral parameters are used to characterize the process of swelling or shrinkage of RBC—the absorbance at 700 nm, the Soret peak height relative to the spectrum background, and the Soret peak wavelength. We show that with an increase in the osmolarity, the absorbance at 700 nm increases and the Soret peak relative height decreases. These changes are related to the changes in the RBC volume and the resulting increase in the hemoglobin intracellular concentration and index of refraction. Confocal microscopy and flow cytometry measurements supported these conclusions. The maximum wavelength of the Soret peak increases with increasing osmolarity due to changes in the oxygenation state of hemoglobin. Using these spectrum parameters, the process of osmosis in RBCs can be followed in real time, but it can also be applied to various processes, leading to changes in the volume and shape of RBCs. Therefore, we conclude that UV–Vis absorption spectrophotometry offers a convenient, easily accessible, and cost-effective method to monitor changes in RBC, which can find applications in the field of drug discovery and diagnostics of RBC and hemoglobin disorders.https://www.mdpi.com/2073-4409/13/7/589red blood cellsosmosishemoglobinabsorption spectrumSoret peak |
| spellingShingle | Miroslav Karabaliev Bilyana Tacheva Boyana Paarvanova Radostina Georgieva Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells Cells red blood cells osmosis hemoglobin absorption spectrum Soret peak |
| title | Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells |
| title_full | Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells |
| title_fullStr | Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells |
| title_full_unstemmed | Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells |
| title_short | Change in Osmotic Pressure Influences the Absorption Spectrum of Hemoglobin inside Red Blood Cells |
| title_sort | change in osmotic pressure influences the absorption spectrum of hemoglobin inside red blood cells |
| topic | red blood cells osmosis hemoglobin absorption spectrum Soret peak |
| url | https://www.mdpi.com/2073-4409/13/7/589 |
| work_keys_str_mv | AT miroslavkarabaliev changeinosmoticpressureinfluencestheabsorptionspectrumofhemoglobininsideredbloodcells AT bilyanatacheva changeinosmoticpressureinfluencestheabsorptionspectrumofhemoglobininsideredbloodcells AT boyanapaarvanova changeinosmoticpressureinfluencestheabsorptionspectrumofhemoglobininsideredbloodcells AT radostinageorgieva changeinosmoticpressureinfluencestheabsorptionspectrumofhemoglobininsideredbloodcells |