Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae
In this study, we screened adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae (E. rhusiopathiae). Inactivated cells of E. rhusiopathiae strain HG-1 were prepared as the antigen in five adjuvanted inactivated vaccines, including a mineral-oil-adjuvanted vaccine (Oli vaccine), a...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.922867/full |
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| author | Li-Jun Guan Li-Jun Guan Shi-Xuan Pei Ji-Jian Song Peng-Fei Zhan Yi-Nong Han Yun Xue Ke Ding Zhan-Qin Zhao |
| author_facet | Li-Jun Guan Li-Jun Guan Shi-Xuan Pei Ji-Jian Song Peng-Fei Zhan Yi-Nong Han Yun Xue Ke Ding Zhan-Qin Zhao |
| author_sort | Li-Jun Guan |
| collection | DOAJ |
| description | In this study, we screened adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae (E. rhusiopathiae). Inactivated cells of E. rhusiopathiae strain HG-1 were prepared as the antigen in five adjuvanted inactivated vaccines, including a mineral-oil-adjuvanted vaccine (Oli vaccine), aluminum-hydroxide-gel-adjuvanted vaccine (Alh vaccine), ISA201-biphasic-oil-emulsion-adjuvanted vaccine (ISA201 vaccine), GEL02-water-soluble-polymer-adjuvanted vaccine (GEL vaccine), and IMS1313-water-soluble-nanoparticle-adjuvanted vaccine (IMS1313 vaccine). The safety test results of subcutaneous inoculation in mice showed that Oli vaccine had the most severe side effects, with a combined score of 35, followed by the ISA201 vaccine (25 points), Alh vaccine (20 points), GEL vaccine (10 points), and IMS1313 vaccine (10 points). A dose of 1.5LD50 of strain HG-1 was used to challenge the mice intraperitoneally, 14 days after their second immunization. The protective efficacy of Oli vaccine and Alh vaccine was 100% (8/8), whereas that of the other three adjuvanted vaccines was 88% (7/8). Challenge with 2.5LD50 of strain HG-1 resulted in a 100% survival rate, demonstrating the 100% protective efficacy of the Oli vaccine, followed by the GEL vaccine (71%, 5/7), IMS1313 vaccine (57%, 4/7), ISA201 vaccine (43%, 3/7), and Alh vaccine (29%, 2/7). Challenge with 4LD50 of strain HG-1 showed 100% (7/7) protective efficacy of the Oli vaccine and 71% (5/7) protective efficacy of the GEL vaccine, whereas the protective efficacy of other three adjuvanted vaccine was 14% (1/7). The Alh and GEL vaccines were selected for comparative tests in piglets, and both caused minor side effects. A second immunization with these two adjuvanted vaccines conferred 60 and 100% protective efficacy, respectively, after the piglets were challenged via an ear vein with 8LD100 of strain HG-1. After challenge with 16LD100 of strain HG-1, the Alh and GEL vaccines showed 40% and 100% protective efficacy, respectively. Our results suggested that GEL is the optimal adjuvant for an inactivated vaccine against E. rhusiopathiae. |
| first_indexed | 2024-12-10T09:06:21Z |
| format | Article |
| id | doaj.art-7ae272c24da0421890d31a2a6abaae0c |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-10T09:06:21Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-7ae272c24da0421890d31a2a6abaae0c2022-12-22T01:55:08ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-07-01910.3389/fvets.2022.922867922867Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiaeLi-Jun Guan0Li-Jun Guan1Shi-Xuan Pei2Ji-Jian Song3Peng-Fei Zhan4Yi-Nong Han5Yun Xue6Ke Ding7Zhan-Qin Zhao8Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaCollege of Veterinary Medicine, Jilin University, Changchun, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaIn this study, we screened adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae (E. rhusiopathiae). Inactivated cells of E. rhusiopathiae strain HG-1 were prepared as the antigen in five adjuvanted inactivated vaccines, including a mineral-oil-adjuvanted vaccine (Oli vaccine), aluminum-hydroxide-gel-adjuvanted vaccine (Alh vaccine), ISA201-biphasic-oil-emulsion-adjuvanted vaccine (ISA201 vaccine), GEL02-water-soluble-polymer-adjuvanted vaccine (GEL vaccine), and IMS1313-water-soluble-nanoparticle-adjuvanted vaccine (IMS1313 vaccine). The safety test results of subcutaneous inoculation in mice showed that Oli vaccine had the most severe side effects, with a combined score of 35, followed by the ISA201 vaccine (25 points), Alh vaccine (20 points), GEL vaccine (10 points), and IMS1313 vaccine (10 points). A dose of 1.5LD50 of strain HG-1 was used to challenge the mice intraperitoneally, 14 days after their second immunization. The protective efficacy of Oli vaccine and Alh vaccine was 100% (8/8), whereas that of the other three adjuvanted vaccines was 88% (7/8). Challenge with 2.5LD50 of strain HG-1 resulted in a 100% survival rate, demonstrating the 100% protective efficacy of the Oli vaccine, followed by the GEL vaccine (71%, 5/7), IMS1313 vaccine (57%, 4/7), ISA201 vaccine (43%, 3/7), and Alh vaccine (29%, 2/7). Challenge with 4LD50 of strain HG-1 showed 100% (7/7) protective efficacy of the Oli vaccine and 71% (5/7) protective efficacy of the GEL vaccine, whereas the protective efficacy of other three adjuvanted vaccine was 14% (1/7). The Alh and GEL vaccines were selected for comparative tests in piglets, and both caused minor side effects. A second immunization with these two adjuvanted vaccines conferred 60 and 100% protective efficacy, respectively, after the piglets were challenged via an ear vein with 8LD100 of strain HG-1. After challenge with 16LD100 of strain HG-1, the Alh and GEL vaccines showed 40% and 100% protective efficacy, respectively. Our results suggested that GEL is the optimal adjuvant for an inactivated vaccine against E. rhusiopathiae.https://www.frontiersin.org/articles/10.3389/fvets.2022.922867/fullErysipelothrix rhusiopathiaeswinevaccineadjuvantprotective efficacy |
| spellingShingle | Li-Jun Guan Li-Jun Guan Shi-Xuan Pei Ji-Jian Song Peng-Fei Zhan Yi-Nong Han Yun Xue Ke Ding Zhan-Qin Zhao Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae Frontiers in Veterinary Science Erysipelothrix rhusiopathiae swine vaccine adjuvant protective efficacy |
| title | Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae |
| title_full | Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae |
| title_fullStr | Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae |
| title_full_unstemmed | Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae |
| title_short | Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae |
| title_sort | screening immune adjuvants for an inactivated vaccine against erysipelothrix rhusiopathiae |
| topic | Erysipelothrix rhusiopathiae swine vaccine adjuvant protective efficacy |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.922867/full |
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