Marker-Assisted Selection to Pyramid Nematode Resistance and the High Oleic Trait in Peanut

The dynamic challenges of peanut ( L.) farming demand a quick response from breeders to develop new cultivars, a process that can be aided by the application of molecular markers. With the goal to pyramid nematode resistance and the trait for high oleic:linoleic acid (high O:L) ratio in seeds, nemat...

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Bibliographic Details
Main Authors: Y. Chu, C. L. Wu, C. C. Holbrook, B. L. Tillman, G. Person, P. Ozias-Akins
Format: Article
Language:English
Published: Wiley 2011-07-01
Series:The Plant Genome
Online Access:https://dl.sciencesocieties.org/publications/tpg/articles/4/2/110
Description
Summary:The dynamic challenges of peanut ( L.) farming demand a quick response from breeders to develop new cultivars, a process that can be aided by the application of molecular markers. With the goal to pyramid nematode resistance and the trait for high oleic:linoleic acid (high O:L) ratio in seeds, nematode-resistant cultivar Tifguard was used as the recurrent female parent and high O:L cultivars Georgia-02C and Florida-07 were used as donor parents for the high O:L trait. ‘Tifguard High O/L’ was generated through three rounds of accelerated backcrossing using BCF progenies selected with molecular markers for these two traits as the pollen donors. Selfed BCF plants yielded marker-homozygous individuals identified as Tifguard High O/L, compressing the hybridization and selection phases of the cultivar development process to less than 3 yr. The accuracy of marker-assisted selection (MAS) was confirmed by phenotyping a subset of F populations from both parental combinations. Once additional molecular markers linked with traits of interest are designed to be compatible with high-throughput screening platforms, MAS will be more widely integrated into peanut breeding programs.
ISSN:1940-3372