SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23
Abstract Objective This study was designed to investigate the regulatory effects of kinesin family member (KIF) 23 on anaplastic thyroid cancer (ATC) cell viability and migration and the underlying mechanism. Methods Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-024-11965-9 |
| _version_ | 1797274308389634048 |
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| author | Yongkang Wu Weijie Chen Huilai Miao Tuo Xu |
| author_facet | Yongkang Wu Weijie Chen Huilai Miao Tuo Xu |
| author_sort | Yongkang Wu |
| collection | DOAJ |
| description | Abstract Objective This study was designed to investigate the regulatory effects of kinesin family member (KIF) 23 on anaplastic thyroid cancer (ATC) cell viability and migration and the underlying mechanism. Methods Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to analyze the levels of KIF23 in ATC cells. Besides, the effects of KIF23 and sirtuin (SIRT) 7 on the viability and migration of ATC cells were detected using cell counting kit-8, transwell and wound healing assays. The interaction between SIRT7 and KIF23 was evaluated by co-immunoprecipitation (Co-IP) assay. The succinylation (succ) of KIF23 was analyzed by western blot. Results The KIF23 expression was upregulated in ATC cells. Silencing of KIF23 suppressed the viability and migration of 8505C and BCPAP cells. The KIF23-succ level was decreased in ATC cells. SIRT7 interacted with KIF23 to inhibit the succinylation of KIF23 at K537 site in human embryonic kidney (HEK)-293T cells. Overexpression of SIRT7 enhanced the protein stability of KIF23 in HEK-293T cells. Besides, overexpression of KIF23 promoted the viability and migration of 8505C and BCPAP cells, which was partly blocked by silenced SIRT7. Conclusions SIRT7 promoted the proliferation and migration of ATC cells by regulating the desuccinylation of KIF23. |
| first_indexed | 2024-03-07T14:56:32Z |
| format | Article |
| id | doaj.art-7ae6ea1058f04b218a8bbfa62431066c |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-07T14:56:32Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-7ae6ea1058f04b218a8bbfa62431066c2024-03-05T19:24:05ZengBMCBMC Cancer1471-24072024-02-0124111010.1186/s12885-024-11965-9SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23Yongkang Wu0Weijie Chen1Huilai Miao2Tuo Xu3Department of Vascular and Thyroid Surgery, The Affiliated Hospital of Guangdong Medical UniversityDepartment of Vascular and Thyroid Surgery, The Affiliated Hospital of Guangdong Medical UniversityDepartment of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangdong Medical UniversityDepartment of Vascular and Thyroid Surgery, The Affiliated Hospital of Guangdong Medical UniversityAbstract Objective This study was designed to investigate the regulatory effects of kinesin family member (KIF) 23 on anaplastic thyroid cancer (ATC) cell viability and migration and the underlying mechanism. Methods Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to analyze the levels of KIF23 in ATC cells. Besides, the effects of KIF23 and sirtuin (SIRT) 7 on the viability and migration of ATC cells were detected using cell counting kit-8, transwell and wound healing assays. The interaction between SIRT7 and KIF23 was evaluated by co-immunoprecipitation (Co-IP) assay. The succinylation (succ) of KIF23 was analyzed by western blot. Results The KIF23 expression was upregulated in ATC cells. Silencing of KIF23 suppressed the viability and migration of 8505C and BCPAP cells. The KIF23-succ level was decreased in ATC cells. SIRT7 interacted with KIF23 to inhibit the succinylation of KIF23 at K537 site in human embryonic kidney (HEK)-293T cells. Overexpression of SIRT7 enhanced the protein stability of KIF23 in HEK-293T cells. Besides, overexpression of KIF23 promoted the viability and migration of 8505C and BCPAP cells, which was partly blocked by silenced SIRT7. Conclusions SIRT7 promoted the proliferation and migration of ATC cells by regulating the desuccinylation of KIF23.https://doi.org/10.1186/s12885-024-11965-9Anaplastic thyroid cancerKIF23SIRT7SuccinylationProliferationMigration |
| spellingShingle | Yongkang Wu Weijie Chen Huilai Miao Tuo Xu SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23 BMC Cancer Anaplastic thyroid cancer KIF23 SIRT7 Succinylation Proliferation Migration |
| title | SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23 |
| title_full | SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23 |
| title_fullStr | SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23 |
| title_full_unstemmed | SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23 |
| title_short | SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23 |
| title_sort | sirt7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of kif23 |
| topic | Anaplastic thyroid cancer KIF23 SIRT7 Succinylation Proliferation Migration |
| url | https://doi.org/10.1186/s12885-024-11965-9 |
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