Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression.
Increasing evidence has accumulated showing the role of APOBEC3G (A3G) and 3F (A3F) in the control of HIV-1 replication and disease progression in humans. However, very few studies have been conducted in HIV-infected children. Here, we analyzed the levels of A3G and A3F expression and induced G-to-A...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3163681?pdf=render |
| _version_ | 1818201697746419712 |
|---|---|
| author | Nívea D Amoêdo Adriana O Afonso Sílvia M Cunha Ricardo H Oliveira Elizabeth S Machado Marcelo A Soares |
| author_facet | Nívea D Amoêdo Adriana O Afonso Sílvia M Cunha Ricardo H Oliveira Elizabeth S Machado Marcelo A Soares |
| author_sort | Nívea D Amoêdo |
| collection | DOAJ |
| description | Increasing evidence has accumulated showing the role of APOBEC3G (A3G) and 3F (A3F) in the control of HIV-1 replication and disease progression in humans. However, very few studies have been conducted in HIV-infected children. Here, we analyzed the levels of A3G and A3F expression and induced G-to-A hypermutation in a group of children with distinct profiles of disease progression.Perinatally HIV-infected children were classified as progressors or long-term non-progressors according to criteria based on HIV viral load and CD4 T-cell counts over time. A group of uninfected control children were also enrolled in the study. PBMC proviral DNA was assessed for G-to-A hypermutation, whereas A3G and A3F mRNA were isolated and quantified through TaqMan® real-time PCR. No correlation was observed between disease progression and A3G/A3F expression or hypermutation levels. Although all children analyzed showed higher expression levels of A3G compared to A3F (an average fold of 5 times), a surprisingly high A3F-related hypermutation rate was evidenced in the cohort, irrespective of the child's disease progression profile.Our results contribute to the current controversy as to whether HIV disease progression is related to A3G/A3F enzymatic activity. To our knowledge, this is the first study analyzing A3G/F expression in HIV-infected children, and it may pave the way to a better understanding of the host factors governing HIV disease in the pediatric setting. |
| first_indexed | 2024-12-12T02:57:41Z |
| format | Article |
| id | doaj.art-7ae76785748e43e88cec25077cc572c7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T02:57:41Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7ae76785748e43e88cec25077cc572c72022-12-22T00:40:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2411810.1371/journal.pone.0024118Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression.Nívea D AmoêdoAdriana O AfonsoSílvia M CunhaRicardo H OliveiraElizabeth S MachadoMarcelo A SoaresIncreasing evidence has accumulated showing the role of APOBEC3G (A3G) and 3F (A3F) in the control of HIV-1 replication and disease progression in humans. However, very few studies have been conducted in HIV-infected children. Here, we analyzed the levels of A3G and A3F expression and induced G-to-A hypermutation in a group of children with distinct profiles of disease progression.Perinatally HIV-infected children were classified as progressors or long-term non-progressors according to criteria based on HIV viral load and CD4 T-cell counts over time. A group of uninfected control children were also enrolled in the study. PBMC proviral DNA was assessed for G-to-A hypermutation, whereas A3G and A3F mRNA were isolated and quantified through TaqMan® real-time PCR. No correlation was observed between disease progression and A3G/A3F expression or hypermutation levels. Although all children analyzed showed higher expression levels of A3G compared to A3F (an average fold of 5 times), a surprisingly high A3F-related hypermutation rate was evidenced in the cohort, irrespective of the child's disease progression profile.Our results contribute to the current controversy as to whether HIV disease progression is related to A3G/A3F enzymatic activity. To our knowledge, this is the first study analyzing A3G/F expression in HIV-infected children, and it may pave the way to a better understanding of the host factors governing HIV disease in the pediatric setting.http://europepmc.org/articles/PMC3163681?pdf=render |
| spellingShingle | Nívea D Amoêdo Adriana O Afonso Sílvia M Cunha Ricardo H Oliveira Elizabeth S Machado Marcelo A Soares Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression. PLoS ONE |
| title | Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression. |
| title_full | Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression. |
| title_fullStr | Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression. |
| title_full_unstemmed | Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression. |
| title_short | Expression of APOBEC3G/3F and G-to-A hypermutation levels in HIV-1-infected children with different profiles of disease progression. |
| title_sort | expression of apobec3g 3f and g to a hypermutation levels in hiv 1 infected children with different profiles of disease progression |
| url | http://europepmc.org/articles/PMC3163681?pdf=render |
| work_keys_str_mv | AT niveadamoedo expressionofapobec3g3fandgtoahypermutationlevelsinhiv1infectedchildrenwithdifferentprofilesofdiseaseprogression AT adrianaoafonso expressionofapobec3g3fandgtoahypermutationlevelsinhiv1infectedchildrenwithdifferentprofilesofdiseaseprogression AT silviamcunha expressionofapobec3g3fandgtoahypermutationlevelsinhiv1infectedchildrenwithdifferentprofilesofdiseaseprogression AT ricardoholiveira expressionofapobec3g3fandgtoahypermutationlevelsinhiv1infectedchildrenwithdifferentprofilesofdiseaseprogression AT elizabethsmachado expressionofapobec3g3fandgtoahypermutationlevelsinhiv1infectedchildrenwithdifferentprofilesofdiseaseprogression AT marceloasoares expressionofapobec3g3fandgtoahypermutationlevelsinhiv1infectedchildrenwithdifferentprofilesofdiseaseprogression |