A Mini-ISY100 Transposon Delivery System Effective in γ Proteobacteria

A Mini-ISY100 Transposon Delivery System Effective in γ Proteobacteria

Transposons are invaluable biological tools for the genetic manipulation of microorganisms. ISY100 from Synechocystis sp. PCC6803 is a member of the Tc1/mariner/IS630 superfamily, and is characterized by high transposition efficiency and a strong preference for TA target sequences. In this paper, we...

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Bibliographic Details
Main Authors: Emanuele Conte, Linda Mende, Ian Grainge, Sean D. Colloms
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Microbiology
Subjects:
ISY100
transposon mutagenesis
knock-out library
ΦC31 integrase
cytochrome c
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00280/full

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