Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond
Late spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [<i>Prunus amygdalus</i> Batsch syn <i>P. dulcis</i> (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/12/527 |
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| author | Beatriz Bielsa Jorge Israel Ávila-Alonso Ángel Fernández i Martí Jérôme Grimplet María José Rubio-Cabetas |
| author_facet | Beatriz Bielsa Jorge Israel Ávila-Alonso Ángel Fernández i Martí Jérôme Grimplet María José Rubio-Cabetas |
| author_sort | Beatriz Bielsa |
| collection | DOAJ |
| description | Late spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [<i>Prunus amygdalus</i> Batsch syn <i>P. dulcis</i> (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order to identify key genes favoring cold tolerance in almonds, branches from three late-blooming genotypes: ‘Guara’, ‘Soleta’ and ‘Belona’ were exposed at −4 °C during 24 h in a constant climate chamber. Phenotype analysis showed that ‘Guara’ and ‘Soleta’ had a greater acclimation capacity to cold than ‘Belona’. The qRT-PCR BioMark System technology was used to monitor the relative expression of 30 candidate genes with a potential relation to cold response, which are either involved in the ICE-CBF-COR pathway or the independent CBF pathway, and also genes not yet characterized or with unknown function in almond genome. Differences in the gene expression profiles were found among the three studied genotypes and the three time-points of cold exposure (0, 2 and 24 h). <i>BBX20</i> and <i>CLO</i> genes behaved as differentiator genes between tolerant and susceptible genotypes in cold stress response in almond pistils. In addition, the differences of expression among the tolerant genotypes suggested the intervention of different mechanisms responding to cold stress in almonds. |
| first_indexed | 2024-03-10T03:59:34Z |
| format | Article |
| id | doaj.art-7ae88f6c53b64bccac013fc5802701dc |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T03:59:34Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-7ae88f6c53b64bccac013fc5802701dc2023-11-23T08:37:45ZengMDPI AGHorticulturae2311-75242021-11-0171252710.3390/horticulturae7120527Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in AlmondBeatriz Bielsa0Jorge Israel Ávila-Alonso1Ángel Fernández i Martí2Jérôme Grimplet3María José Rubio-Cabetas4Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, SpainUnidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, SpainDepartment of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA 94720, USAUnidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, SpainUnidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, SpainLate spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [<i>Prunus amygdalus</i> Batsch syn <i>P. dulcis</i> (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order to identify key genes favoring cold tolerance in almonds, branches from three late-blooming genotypes: ‘Guara’, ‘Soleta’ and ‘Belona’ were exposed at −4 °C during 24 h in a constant climate chamber. Phenotype analysis showed that ‘Guara’ and ‘Soleta’ had a greater acclimation capacity to cold than ‘Belona’. The qRT-PCR BioMark System technology was used to monitor the relative expression of 30 candidate genes with a potential relation to cold response, which are either involved in the ICE-CBF-COR pathway or the independent CBF pathway, and also genes not yet characterized or with unknown function in almond genome. Differences in the gene expression profiles were found among the three studied genotypes and the three time-points of cold exposure (0, 2 and 24 h). <i>BBX20</i> and <i>CLO</i> genes behaved as differentiator genes between tolerant and susceptible genotypes in cold stress response in almond pistils. In addition, the differences of expression among the tolerant genotypes suggested the intervention of different mechanisms responding to cold stress in almonds.https://www.mdpi.com/2311-7524/7/12/527cold damagegene expressionlow temperaturesphenotyping<i>Prunus</i>spring frosts |
| spellingShingle | Beatriz Bielsa Jorge Israel Ávila-Alonso Ángel Fernández i Martí Jérôme Grimplet María José Rubio-Cabetas Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond Horticulturae cold damage gene expression low temperatures phenotyping <i>Prunus</i> spring frosts |
| title | Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond |
| title_full | Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond |
| title_fullStr | Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond |
| title_full_unstemmed | Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond |
| title_short | Gene Expression Analysis in Cold Stress Conditions Reveals <i>BBX20</i> and <i>CLO</i> as Potential Biomarkers for Cold Tolerance in Almond |
| title_sort | gene expression analysis in cold stress conditions reveals i bbx20 i and i clo i as potential biomarkers for cold tolerance in almond |
| topic | cold damage gene expression low temperatures phenotyping <i>Prunus</i> spring frosts |
| url | https://www.mdpi.com/2311-7524/7/12/527 |
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