Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions
Background: The selection of compatible human leukocyte antigen platelets has been associated with improved platelet increments. Therefore, an effective strategy would be the selection of donors who are genetically compatible according to the human leukocyte antigen system. Nonetheless, this is cost...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Hematology, Transfusion and Cell Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137918300828 |
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| author | Millena Gomes Ferreira Fernanda Bernadelli De Vito Aline Aparecida Ferreira Carolina Bonet Bub Fernando Antônio Vinhal dos Santos Adilson Botelho Filho Sheila Soares Silva Helio Moraes-Souza |
| author_facet | Millena Gomes Ferreira Fernanda Bernadelli De Vito Aline Aparecida Ferreira Carolina Bonet Bub Fernando Antônio Vinhal dos Santos Adilson Botelho Filho Sheila Soares Silva Helio Moraes-Souza |
| author_sort | Millena Gomes Ferreira |
| collection | DOAJ |
| description | Background: The selection of compatible human leukocyte antigen platelets has been associated with improved platelet increments. Therefore, an effective strategy would be the selection of donors who are genetically compatible according to the human leukocyte antigen system. Nonetheless, this is costly as it concerns a highly polymorphic system, which requires a large bank of genotyped donors. Methods: This study evaluated the feasibility of virtual crossmatching using EpVix software, which simplifies the identification of compatible donors or donors with acceptable incompatibilities. Results: Forty-three oncohematological patients were evaluated, in 96 platelet transfusion episodes with 16.3% of the patients being found to be refractory to platelet transfusions. Eight alloimmunized, multitransfused patients were selected to evaluate human leukocyte antigen compatibility against a bank of 336 platelet donors. At least partially compatible donors were found for all patients. The number of compatible donors was found to be inversely proportional to the human leukocyte antigen-panel reactive antibody score of each patient. It was noted that five patients with scores of 15% or less had at least 190 compatible donors; four fully compatible donors were found for two other patients with scores greater than 80% and only one patient (score of 93%) did not have a fully compatible donor. However, for this last patient, 40 donors were partially compatible according to the software. Conclusion: The results showed the effectiveness of the use of the EpVix tool to identify potential platelet donors for multitransfused and/or alloimmunized patients, even with a small number of human leukocyte antigen genotyped donors available. Keywords: Platelet transfusion, HLA antigens, Crossmatching, EpVix software |
| first_indexed | 2024-04-12T09:36:50Z |
| format | Article |
| id | doaj.art-7aeadcf2fddf4fd0963ea9ff496a476b |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-04-12T09:36:50Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-7aeadcf2fddf4fd0963ea9ff496a476b2022-12-22T03:38:12ZengElsevierHematology, Transfusion and Cell Therapy2531-13792018-10-01404298304Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusionsMillena Gomes Ferreira0Fernanda Bernadelli De Vito1Aline Aparecida Ferreira2Carolina Bonet Bub3Fernando Antônio Vinhal dos Santos4Adilson Botelho Filho5Sheila Soares Silva6Helio Moraes-Souza7Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, BrazilUniversidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, BrazilUniversidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, Brazil; Hemocentro Regional de Uberaba, Uberaba, MG, BrazilHospital Israelita Albert Einstein, São Paulo, SP, BrazilLaboratório de Imunologia e Transplantes de Uberlândia, Uberlândia, MG, BrazilHemococentro Regional de Uberlândia, Uberlandia, MG, BrazilUniversidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, Brazil; Hemocentro Regional de Uberaba, Uberaba, MG, BrazilUniversidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, Brazil; Hemococentro Regional de Uberlândia, Uberlandia, MG, Brazil; Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil; Corresponding author at: Avenida Getúlio Guaritá, 250 – Bairro Abadia, 38025-440 Uberaba, Minas Gerais, Brazil.Background: The selection of compatible human leukocyte antigen platelets has been associated with improved platelet increments. Therefore, an effective strategy would be the selection of donors who are genetically compatible according to the human leukocyte antigen system. Nonetheless, this is costly as it concerns a highly polymorphic system, which requires a large bank of genotyped donors. Methods: This study evaluated the feasibility of virtual crossmatching using EpVix software, which simplifies the identification of compatible donors or donors with acceptable incompatibilities. Results: Forty-three oncohematological patients were evaluated, in 96 platelet transfusion episodes with 16.3% of the patients being found to be refractory to platelet transfusions. Eight alloimmunized, multitransfused patients were selected to evaluate human leukocyte antigen compatibility against a bank of 336 platelet donors. At least partially compatible donors were found for all patients. The number of compatible donors was found to be inversely proportional to the human leukocyte antigen-panel reactive antibody score of each patient. It was noted that five patients with scores of 15% or less had at least 190 compatible donors; four fully compatible donors were found for two other patients with scores greater than 80% and only one patient (score of 93%) did not have a fully compatible donor. However, for this last patient, 40 donors were partially compatible according to the software. Conclusion: The results showed the effectiveness of the use of the EpVix tool to identify potential platelet donors for multitransfused and/or alloimmunized patients, even with a small number of human leukocyte antigen genotyped donors available. Keywords: Platelet transfusion, HLA antigens, Crossmatching, EpVix softwarehttp://www.sciencedirect.com/science/article/pii/S2531137918300828 |
| spellingShingle | Millena Gomes Ferreira Fernanda Bernadelli De Vito Aline Aparecida Ferreira Carolina Bonet Bub Fernando Antônio Vinhal dos Santos Adilson Botelho Filho Sheila Soares Silva Helio Moraes-Souza Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions Hematology, Transfusion and Cell Therapy |
| title | Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions |
| title_full | Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions |
| title_fullStr | Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions |
| title_full_unstemmed | Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions |
| title_short | Applicability of an instrument to identify human leukocyte antigen-compatible donors for platelet transfusions |
| title_sort | applicability of an instrument to identify human leukocyte antigen compatible donors for platelet transfusions |
| url | http://www.sciencedirect.com/science/article/pii/S2531137918300828 |
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