Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver
The formation of product by ethanolamine phosphotransferases (EC 2.7.8.1) and cholinephosphotransferases (EC 2.7.8.2) in microsomal fractions from brains and livers of mature rats is increased several fold by 1,2-diacyl-sn-glycerols. With the addition of 1-alkyl-2-acyl-sn-glycerols, we have found an...
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| Format: | Article |
| Language: | English |
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Elsevier
1977-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520417146 |
| _version_ | 1818716141679280128 |
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| author | A Radominska-Pyrek J Strosznajder Z Dabrowiecki G Goracci T Chojnacki L A Horrocks |
| author_facet | A Radominska-Pyrek J Strosznajder Z Dabrowiecki G Goracci T Chojnacki L A Horrocks |
| author_sort | A Radominska-Pyrek |
| collection | DOAJ |
| description | The formation of product by ethanolamine phosphotransferases (EC 2.7.8.1) and cholinephosphotransferases (EC 2.7.8.2) in microsomal fractions from brains and livers of mature rats is increased several fold by 1,2-diacyl-sn-glycerols. With the addition of 1-alkyl-2-acyl-sn-glycerols, we have found an 11-fold increase with brain microsomes and a 20-fold increase with lvier microsomes in the synthesis of choline ether lipids (1-alkyl-2-acyl- and 1-alk-1′-enyl-2-acyl-sn-glycero-3-phosphorylcholines). For the synthesis of ethanolamine ether lipids (1-alkyl-2-acyl and 1-alk-1′-enyl-2-acyl-sn-glycero-3-phosphorylethanolamines), the stimulation of alkylacylglycerols was 7-fold for brain microsomes and 18-fold for liver microsomes. The alkylacyl glycerols (8 mM) also inhibited the synthesis of diacyl phosphoglycerides by 44 to 65%, indicating that the same ethanolaminephosphotransferases and cholinephosphotransferases are utilized for the synthesis of alkylacyl phosphoglycerides and diacyl phosphoglycerides. A desaturation of the alkyl groups may take place in the same reaction mixture. The rate of incorporation of phosphorylcholine into alkenylacyl glycerophosphorylcholines (choline plasmalogens) with alkylacylglycerols, cytidine diphosphate choline, and liver microsomes was 15 nmoles per mg protein per hour. The in vitro synthesis of choline plasmalogens with alkylacylglycerols had not been observed previously. The corresponding rate of incorporation of phosphorylethanolamine into ethanolamine plasmalogens was 10 nmoles per mg protein per hour, a value greater than any of the previously reported values for ethanolamine plasmalogen formation from alkylacyl glycerophosphorylethanolamines. |
| first_indexed | 2024-12-17T19:14:33Z |
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| id | doaj.art-7aec46b65d5e4451a8d05a599a037c33 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T19:14:33Z |
| publishDate | 1977-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7aec46b65d5e4451a8d05a599a037c332022-12-21T21:35:47ZengElsevierJournal of Lipid Research0022-22751977-01-011815358Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liverA Radominska-Pyrek0J Strosznajder1Z Dabrowiecki2G Goracci3T Chojnacki4L A Horrocks5Polish Academy of Sciences, Warsaw, Poland; Department of Physiological Chemistry, the Ohio State University, Columbus, OH 43210; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36. 02-532 Warsaw, PolandPolish Academy of Sciences, Warsaw, Poland; Department of Physiological Chemistry, the Ohio State University, Columbus, OH 43210; Experimental and Clinical Medical Research Center, Polish Academy of Sciences, Warsaw, PolandPolish Academy of Sciences, Warsaw, Poland; Department of Physiological Chemistry, the Ohio State University, Columbus, OH 43210; Experimental and Clinical Medical Research Center, Polish Academy of Sciences, Warsaw, PolandPolish Academy of Sciences, Warsaw, Poland; Department of Physiological Chemistry, the Ohio State University, Columbus, OH 43210; Department of Physiological Chemistry, The Ohio State University, Columbus, OH 43210Polish Academy of Sciences, Warsaw, Poland; Department of Physiological Chemistry, the Ohio State University, Columbus, OH 43210; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36. 02-532 Warsaw, PolandPolish Academy of Sciences, Warsaw, Poland; Department of Physiological Chemistry, the Ohio State University, Columbus, OH 43210; Department of Physiological Chemistry, The Ohio State University, Columbus, OH 43210The formation of product by ethanolamine phosphotransferases (EC 2.7.8.1) and cholinephosphotransferases (EC 2.7.8.2) in microsomal fractions from brains and livers of mature rats is increased several fold by 1,2-diacyl-sn-glycerols. With the addition of 1-alkyl-2-acyl-sn-glycerols, we have found an 11-fold increase with brain microsomes and a 20-fold increase with lvier microsomes in the synthesis of choline ether lipids (1-alkyl-2-acyl- and 1-alk-1′-enyl-2-acyl-sn-glycero-3-phosphorylcholines). For the synthesis of ethanolamine ether lipids (1-alkyl-2-acyl and 1-alk-1′-enyl-2-acyl-sn-glycero-3-phosphorylethanolamines), the stimulation of alkylacylglycerols was 7-fold for brain microsomes and 18-fold for liver microsomes. The alkylacyl glycerols (8 mM) also inhibited the synthesis of diacyl phosphoglycerides by 44 to 65%, indicating that the same ethanolaminephosphotransferases and cholinephosphotransferases are utilized for the synthesis of alkylacyl phosphoglycerides and diacyl phosphoglycerides. A desaturation of the alkyl groups may take place in the same reaction mixture. The rate of incorporation of phosphorylcholine into alkenylacyl glycerophosphorylcholines (choline plasmalogens) with alkylacylglycerols, cytidine diphosphate choline, and liver microsomes was 15 nmoles per mg protein per hour. The in vitro synthesis of choline plasmalogens with alkylacylglycerols had not been observed previously. The corresponding rate of incorporation of phosphorylethanolamine into ethanolamine plasmalogens was 10 nmoles per mg protein per hour, a value greater than any of the previously reported values for ethanolamine plasmalogen formation from alkylacyl glycerophosphorylethanolamines.http://www.sciencedirect.com/science/article/pii/S0022227520417146cholinephosphotransferase (EC 2.7.8.2)ethanolaminephosphotransferase (EC 2.7.8.1)plasmalogenphosphatidylethanolaminephosphatidylcholinecytidine-5′-diphosphate choline |
| spellingShingle | A Radominska-Pyrek J Strosznajder Z Dabrowiecki G Goracci T Chojnacki L A Horrocks Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver Journal of Lipid Research cholinephosphotransferase (EC 2.7.8.2) ethanolaminephosphotransferase (EC 2.7.8.1) plasmalogen phosphatidylethanolamine phosphatidylcholine cytidine-5′-diphosphate choline |
| title | Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver |
| title_full | Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver |
| title_fullStr | Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver |
| title_full_unstemmed | Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver |
| title_short | Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver |
| title_sort | enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver |
| topic | cholinephosphotransferase (EC 2.7.8.2) ethanolaminephosphotransferase (EC 2.7.8.1) plasmalogen phosphatidylethanolamine phosphatidylcholine cytidine-5′-diphosphate choline |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520417146 |
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