Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study.
Choroidal neovascularization (CNV) of the macular area of the retina is the major cause of severe vision loss in adults. In CNV, after choriocapillaries initially penetrate Bruch's membrane (BrM), invading vessels may regress or expand (CNV initiation). Next, during Early and Late CNV, the expa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC3342931?pdf=render |
| _version_ | 1811205640887992320 |
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| author | Abbas Shirinifard James Alexander Glazier Maciej Swat J Scott Gens Fereydoon Family Yi Jiang Hans E Grossniklaus |
| author_facet | Abbas Shirinifard James Alexander Glazier Maciej Swat J Scott Gens Fereydoon Family Yi Jiang Hans E Grossniklaus |
| author_sort | Abbas Shirinifard |
| collection | DOAJ |
| description | Choroidal neovascularization (CNV) of the macular area of the retina is the major cause of severe vision loss in adults. In CNV, after choriocapillaries initially penetrate Bruch's membrane (BrM), invading vessels may regress or expand (CNV initiation). Next, during Early and Late CNV, the expanding vasculature usually spreads in one of three distinct patterns: in a layer between BrM and the retinal pigment epithelium (sub-RPE or Type 1 CNV), in a layer between the RPE and the photoreceptors (sub-retinal or Type 2 CNV) or in both loci simultaneously (combined pattern or Type 3 CNV). While most studies hypothesize that CNV primarily results from growth-factor effects or holes in BrM, our three-dimensional simulations of multi-cell model of the normal and pathological maculae recapitulate the three growth patterns, under the hypothesis that CNV results from combinations of impairment of: 1) RPE-RPE epithelial junctional adhesion, 2) Adhesion of the RPE basement membrane complex to BrM (RPE-BrM adhesion), and 3) Adhesion of the RPE to the photoreceptor outer segments (RPE-POS adhesion). Our key findings are that when an endothelial tip cell penetrates BrM: 1) RPE with normal epithelial junctions, basal attachment to BrM and apical attachment to POS resists CNV. 2) Small holes in BrM do not, by themselves, initiate CNV. 3) RPE with normal epithelial junctions and normal apical RPE-POS adhesion, but weak adhesion to BrM (e.g. due to lipid accumulation in BrM) results in Early sub-RPE CNV. 4) Normal adhesion of RBaM to BrM, but reduced apical RPE-POS or epithelial RPE-RPE adhesion (e.g. due to inflammation) results in Early sub-retinal CNV. 5) Simultaneous reduction in RPE-RPE epithelial binding and RPE-BrM adhesion results in either sub-RPE or sub-retinal CNV which often progresses to combined pattern CNV. These findings suggest that defects in adhesion dominate CNV initiation and progression. |
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| format | Article |
| id | doaj.art-7aec8a87954f41b3b0c14dd03743282c |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-04-12T03:35:36Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Computational Biology |
| spelling | doaj.art-7aec8a87954f41b3b0c14dd03743282c2022-12-22T03:49:26ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582012-01-0185e100244010.1371/journal.pcbi.1002440Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study.Abbas ShirinifardJames Alexander GlazierMaciej SwatJ Scott GensFereydoon FamilyYi JiangHans E GrossniklausChoroidal neovascularization (CNV) of the macular area of the retina is the major cause of severe vision loss in adults. In CNV, after choriocapillaries initially penetrate Bruch's membrane (BrM), invading vessels may regress or expand (CNV initiation). Next, during Early and Late CNV, the expanding vasculature usually spreads in one of three distinct patterns: in a layer between BrM and the retinal pigment epithelium (sub-RPE or Type 1 CNV), in a layer between the RPE and the photoreceptors (sub-retinal or Type 2 CNV) or in both loci simultaneously (combined pattern or Type 3 CNV). While most studies hypothesize that CNV primarily results from growth-factor effects or holes in BrM, our three-dimensional simulations of multi-cell model of the normal and pathological maculae recapitulate the three growth patterns, under the hypothesis that CNV results from combinations of impairment of: 1) RPE-RPE epithelial junctional adhesion, 2) Adhesion of the RPE basement membrane complex to BrM (RPE-BrM adhesion), and 3) Adhesion of the RPE to the photoreceptor outer segments (RPE-POS adhesion). Our key findings are that when an endothelial tip cell penetrates BrM: 1) RPE with normal epithelial junctions, basal attachment to BrM and apical attachment to POS resists CNV. 2) Small holes in BrM do not, by themselves, initiate CNV. 3) RPE with normal epithelial junctions and normal apical RPE-POS adhesion, but weak adhesion to BrM (e.g. due to lipid accumulation in BrM) results in Early sub-RPE CNV. 4) Normal adhesion of RBaM to BrM, but reduced apical RPE-POS or epithelial RPE-RPE adhesion (e.g. due to inflammation) results in Early sub-retinal CNV. 5) Simultaneous reduction in RPE-RPE epithelial binding and RPE-BrM adhesion results in either sub-RPE or sub-retinal CNV which often progresses to combined pattern CNV. These findings suggest that defects in adhesion dominate CNV initiation and progression.http://europepmc.org/articles/PMC3342931?pdf=render |
| spellingShingle | Abbas Shirinifard James Alexander Glazier Maciej Swat J Scott Gens Fereydoon Family Yi Jiang Hans E Grossniklaus Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study. PLoS Computational Biology |
| title | Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study. |
| title_full | Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study. |
| title_fullStr | Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study. |
| title_full_unstemmed | Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study. |
| title_short | Adhesion failures determine the pattern of choroidal neovascularization in the eye: a computer simulation study. |
| title_sort | adhesion failures determine the pattern of choroidal neovascularization in the eye a computer simulation study |
| url | http://europepmc.org/articles/PMC3342931?pdf=render |
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