The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence
Objectives: The aim of this study is to determine the optimal duration of antimicrobial therapy for lower respiratory tract infection (LRTI) in neuromuscular disorder (NMD) patients. Methods: This prospective study included 13 episodes from 9 NMD patients hospitalized for bacterial LRTI. Sputum samp...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971220307517 |
| _version_ | 1819228918091087872 |
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| author | Masaru Kawamura Takayuki Hoshina Masato Ogawa Noboru Yamamoto Kaoru Haro Tokiko Kumadaki Kazumasa Fukuda Koichi Kusuhara |
| author_facet | Masaru Kawamura Takayuki Hoshina Masato Ogawa Noboru Yamamoto Kaoru Haro Tokiko Kumadaki Kazumasa Fukuda Koichi Kusuhara |
| author_sort | Masaru Kawamura |
| collection | DOAJ |
| description | Objectives: The aim of this study is to determine the optimal duration of antimicrobial therapy for lower respiratory tract infection (LRTI) in neuromuscular disorder (NMD) patients. Methods: This prospective study included 13 episodes from 9 NMD patients hospitalized for bacterial LRTI. Sputum samples were collected from these patients during the three consecutive days after their admission. Bacterial cell counts and the proportion of the most predominant bacterium identified by a clone library analysis of the bacterial 16S rRNA gene sequence in the samples obtained before antimicrobial therapy were serially investigated. Results: All episodes were initially treated with ampicillin/sulbactam. In 12 episodes with a therapeutic effect, the bacterial cell counts in the samples obtained on the third day of therapy were significantly lower than those before therapy (P = 0.0013). In most of these episodes, the most predominant bacterium in the sample obtained before therapy was undetectable by the third day of therapy. In the one patient without a therapeutic effect, neither the bacterial cell counts nor the proportion of the most predominant bacterium in the sample obtained before therapy decrease after therapy. Conclusion: Short-term antimicrobial therapy is sufficiently effective for LRTI in NMD patients if the initial therapy is effective. |
| first_indexed | 2024-12-23T11:04:54Z |
| format | Article |
| id | doaj.art-7af2bdc8743d414586572ab1b25cbdb0 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-23T11:04:54Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-7af2bdc8743d414586572ab1b25cbdb02022-12-21T17:49:31ZengElsevierInternational Journal of Infectious Diseases1201-97122020-11-01100396401The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequenceMasaru Kawamura0Takayuki Hoshina1Masato Ogawa2Noboru Yamamoto3Kaoru Haro4Tokiko Kumadaki5Kazumasa Fukuda6Koichi Kusuhara7Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanDepartment of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan; Corresponding author at: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan.Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanDepartment of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanDepartment of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan; Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanDepartment of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanDepartment of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanDepartment of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, JapanObjectives: The aim of this study is to determine the optimal duration of antimicrobial therapy for lower respiratory tract infection (LRTI) in neuromuscular disorder (NMD) patients. Methods: This prospective study included 13 episodes from 9 NMD patients hospitalized for bacterial LRTI. Sputum samples were collected from these patients during the three consecutive days after their admission. Bacterial cell counts and the proportion of the most predominant bacterium identified by a clone library analysis of the bacterial 16S rRNA gene sequence in the samples obtained before antimicrobial therapy were serially investigated. Results: All episodes were initially treated with ampicillin/sulbactam. In 12 episodes with a therapeutic effect, the bacterial cell counts in the samples obtained on the third day of therapy were significantly lower than those before therapy (P = 0.0013). In most of these episodes, the most predominant bacterium in the sample obtained before therapy was undetectable by the third day of therapy. In the one patient without a therapeutic effect, neither the bacterial cell counts nor the proportion of the most predominant bacterium in the sample obtained before therapy decrease after therapy. Conclusion: Short-term antimicrobial therapy is sufficiently effective for LRTI in NMD patients if the initial therapy is effective.http://www.sciencedirect.com/science/article/pii/S1201971220307517Neuromuscular disordersBacterial lower respiratory tract infectionAntimicrobial therapyClone library analysisMicrobiome |
| spellingShingle | Masaru Kawamura Takayuki Hoshina Masato Ogawa Noboru Yamamoto Kaoru Haro Tokiko Kumadaki Kazumasa Fukuda Koichi Kusuhara The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence International Journal of Infectious Diseases Neuromuscular disorders Bacterial lower respiratory tract infection Antimicrobial therapy Clone library analysis Microbiome |
| title | The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence |
| title_full | The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence |
| title_fullStr | The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence |
| title_full_unstemmed | The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence |
| title_short | The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence |
| title_sort | optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16s rrna gene sequence |
| topic | Neuromuscular disorders Bacterial lower respiratory tract infection Antimicrobial therapy Clone library analysis Microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S1201971220307517 |
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