Effects of Daxx on γ-ray induced apoptosis of HeLa cells
We studied the effects of overexpression of deathdomainassociated protein (Daxx) on cell apoptosis under γ-ray radiation. Further, we explored the relationship between Daxx and radiation-induced apoptosis and investigated the mechanism behind such apoptosis. Through transfection, cervicalcancer HeLa...
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| Format: | Article |
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Science Press
2021-10-01
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| Series: | Fushe yanjiu yu fushe gongyi xuebao |
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| Online Access: | http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2021.rrj.39.050302&lang=zh |
| _version_ | 1828009910949904384 |
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| author | TANG Shuangyang DING Shuang SHEN Haiyan WU Hongrong HU Yu LIAO Yaqi XIONG Yuling LI Le |
| author_facet | TANG Shuangyang DING Shuang SHEN Haiyan WU Hongrong HU Yu LIAO Yaqi XIONG Yuling LI Le |
| author_sort | TANG Shuangyang |
| collection | DOAJ |
| description | We studied the effects of overexpression of deathdomainassociated protein (Daxx) on cell apoptosis under γ-ray radiation. Further, we explored the relationship between Daxx and radiation-induced apoptosis and investigated the mechanism behind such apoptosis. Through transfection, cervicalcancer HeLa cells were divided into a control group, an empty plasmid-transfected negative group, and a Daxx-transfected group; the HeLa cells were irradiated with different doses of γ-rays using a cell irradiator. The expression of Daxx protein in cells was detected by Western blot. Cell proliferation was detected by MTT. The relative activity of caspase-8 in cells was detected by ELISA whereas the cell apoptosis was detected by flow cytometry. The results showed that the total number of cells decreased with the increase in absorbed dose, whereas the number of deformed cells increased. Furthermore, the degree of Daxx expression increased with the increase in absorbed dose. Comparing the cells of the Daxx-transfected group with those of the unirradiated control group, we observed statistically significant increments (p<0.01, p<0.05, p<0.05) in the rate of inhibition against cell proliferation, the A405 value, and rate of apoptosis. Hence, overexpression of Daxx promotes radiation-induced apoptosis by upregulating caspase-8 activity in the γ-ray irradiated cells. |
| first_indexed | 2024-04-10T08:46:58Z |
| format | Article |
| id | doaj.art-7af57f005d924978a4fc1fe65e4f46ec |
| institution | Directory Open Access Journal |
| issn | 1000-3436 |
| language | zho |
| last_indexed | 2024-04-10T08:46:58Z |
| publishDate | 2021-10-01 |
| publisher | Science Press |
| record_format | Article |
| series | Fushe yanjiu yu fushe gongyi xuebao |
| spelling | doaj.art-7af57f005d924978a4fc1fe65e4f46ec2023-02-22T07:22:42ZzhoScience PressFushe yanjiu yu fushe gongyi xuebao1000-34362021-10-01395566110.11889/j.1000-3436.2021.rrj.39.0503021000-3436（2021）05-0056-06Effects of Daxx on γ-ray induced apoptosis of HeLa cellsTANG Shuangyang0DING Shuang1SHEN Haiyan2WU Hongrong3HU Yu4LIAO Yaqi5XIONG Yuling6LI Le7Pathogenic Biology Institute, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,University of South China, Hengyang 421001, ChinaPathogenic Biology Institute, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,University of South China, Hengyang 421001, ChinaPathogenic Biology Institute, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,University of South China, Hengyang 421001, ChinaPathogenic Biology Institute, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,University of South China, Hengyang 421001, ChinaSchool of Public Health, University of South China, Hengyang 421001, ChinaPathogenic Biology Institute, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,University of South China, Hengyang 421001, ChinaHengyang Medicine School, University of South China, Hengyang 421001, ChinaSchool of Public Health, University of South China, Hengyang 421001, ChinaWe studied the effects of overexpression of deathdomainassociated protein (Daxx) on cell apoptosis under γ-ray radiation. Further, we explored the relationship between Daxx and radiation-induced apoptosis and investigated the mechanism behind such apoptosis. Through transfection, cervicalcancer HeLa cells were divided into a control group, an empty plasmid-transfected negative group, and a Daxx-transfected group; the HeLa cells were irradiated with different doses of γ-rays using a cell irradiator. The expression of Daxx protein in cells was detected by Western blot. Cell proliferation was detected by MTT. The relative activity of caspase-8 in cells was detected by ELISA whereas the cell apoptosis was detected by flow cytometry. The results showed that the total number of cells decreased with the increase in absorbed dose, whereas the number of deformed cells increased. Furthermore, the degree of Daxx expression increased with the increase in absorbed dose. Comparing the cells of the Daxx-transfected group with those of the unirradiated control group, we observed statistically significant increments (p<0.01, p<0.05, p<0.05) in the rate of inhibition against cell proliferation, the A405 value, and rate of apoptosis. Hence, overexpression of Daxx promotes radiation-induced apoptosis by upregulating caspase-8 activity in the γ-ray irradiated cells.http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2021.rrj.39.050302&lang=zhdeathdomainassociated protein (daxx), apoptosis, ionizing radiation clcr811 |
| spellingShingle | TANG Shuangyang DING Shuang SHEN Haiyan WU Hongrong HU Yu LIAO Yaqi XIONG Yuling LI Le Effects of Daxx on γ-ray induced apoptosis of HeLa cells Fushe yanjiu yu fushe gongyi xuebao deathdomainassociated protein (daxx), apoptosis, ionizing radiation clc r811 |
| title | Effects of Daxx on γ-ray induced apoptosis of HeLa cells |
| title_full | Effects of Daxx on γ-ray induced apoptosis of HeLa cells |
| title_fullStr | Effects of Daxx on γ-ray induced apoptosis of HeLa cells |
| title_full_unstemmed | Effects of Daxx on γ-ray induced apoptosis of HeLa cells |
| title_short | Effects of Daxx on γ-ray induced apoptosis of HeLa cells |
| title_sort | effects of daxx on γ ray induced apoptosis of hela cells |
| topic | deathdomainassociated protein (daxx), apoptosis, ionizing radiation clc r811 |
| url | http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2021.rrj.39.050302&lang=zh |
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