Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study
<i>Background and Objectives</i>: The majority of research on the effects of osteoporosis drugs has measured the bone mineral density (BMD) of the spine and femur through dual-energy X-ray absorptiometry (DEXA) and compared and analyzed the effects of the drugs through changes in the BMD...
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MDPI AG
2022-10-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/58/11/1566 |
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| author | Hwan-Hee Lee Eun-Yong Choi Hyun-Sik Jun Young-Yul Kim |
| author_facet | Hwan-Hee Lee Eun-Yong Choi Hyun-Sik Jun Young-Yul Kim |
| author_sort | Hwan-Hee Lee |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The majority of research on the effects of osteoporosis drugs has measured the bone mineral density (BMD) of the spine and femur through dual-energy X-ray absorptiometry (DEXA) and compared and analyzed the effects of the drugs through changes in the BMD values. This study aims to compare osteoclast and sclerostin expression in osteocytes after risedronate therapy by obtaining femoral heads from patients with hip fractures. <i>Materials and Methods</i>: We obtained the femoral heads of 10 female patients (age: ≥65 years) who received risedronate therapy for at least 1 year through hip arthroplasty during 2019–2021 (risedronate group). Meanwhile, 10 patients who had never received osteoporosis treatment were selected as controls using propensity scores with age, body mass index, and bone density as covariates (control group). While the osteoclast count was evaluated using tartrate-resistant acid phosphatase (TRAP) staining, the sclerostin expression in osteocytes was assessed using immunohistochemistry. Moreover, Western blotting and polymerase chain reaction (PCR) were performed for receptor activation of nuclear factor kappa-Β ligand (RANKL), RANK, osteoprotegerin (OPG), sclerostin, and bone morphogenetic protein-2 (BMP2). <i>Results</i>: TRAP staining revealed significantly more TRAP-positive cells in the control group (131.75 ± 27.16/mm<sup>2</sup>) than in the risedronate group (28.00 ± 8.12/mm<sup>2</sup>). Moreover, sclerostin-positive osteocytes were expressed more in the control group (364.12 ± 28.12/mm<sup>2</sup>) than in the risedronate group (106.93 ± 12.85/mm<sup>2</sup>). Western blotting revealed that the expressions of RANKL, RANK, sclerostin, and BMP2 were higher in the control group than in the risedronate group (<i>p</i> < 0.05). Furthermore, RANK, sclerostin, and OPG protein levels were higher in the control group than in the risedronate group. <i>Conclusions</i>: In this study, the risedronate group demonstrated lower osteoclast activity and sclerostin expression in osteocytes in the femoral head than the control group. |
| first_indexed | 2024-03-09T18:51:56Z |
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| id | doaj.art-7af5c398bf4b4e298471a58d57f5ba69 |
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| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T18:51:56Z |
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| spelling | doaj.art-7af5c398bf4b4e298471a58d57f5ba692023-11-24T05:46:54ZengMDPI AGMedicina1010-660X1648-91442022-10-015811156610.3390/medicina58111566Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative StudyHwan-Hee Lee0Eun-Yong Choi1Hyun-Sik Jun2Young-Yul Kim3Department of Orthopedic Surgery, Daejeon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Daejeon 34943, KoreaDepartment of Orthopedic Surgery, Daejeon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Daejeon 34943, KoreaDepartment of Orthopedic Surgery, Daejeon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Daejeon 34943, KoreaDepartment of Orthopedic Surgery, Daejeon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Daejeon 34943, Korea<i>Background and Objectives</i>: The majority of research on the effects of osteoporosis drugs has measured the bone mineral density (BMD) of the spine and femur through dual-energy X-ray absorptiometry (DEXA) and compared and analyzed the effects of the drugs through changes in the BMD values. This study aims to compare osteoclast and sclerostin expression in osteocytes after risedronate therapy by obtaining femoral heads from patients with hip fractures. <i>Materials and Methods</i>: We obtained the femoral heads of 10 female patients (age: ≥65 years) who received risedronate therapy for at least 1 year through hip arthroplasty during 2019–2021 (risedronate group). Meanwhile, 10 patients who had never received osteoporosis treatment were selected as controls using propensity scores with age, body mass index, and bone density as covariates (control group). While the osteoclast count was evaluated using tartrate-resistant acid phosphatase (TRAP) staining, the sclerostin expression in osteocytes was assessed using immunohistochemistry. Moreover, Western blotting and polymerase chain reaction (PCR) were performed for receptor activation of nuclear factor kappa-Β ligand (RANKL), RANK, osteoprotegerin (OPG), sclerostin, and bone morphogenetic protein-2 (BMP2). <i>Results</i>: TRAP staining revealed significantly more TRAP-positive cells in the control group (131.75 ± 27.16/mm<sup>2</sup>) than in the risedronate group (28.00 ± 8.12/mm<sup>2</sup>). Moreover, sclerostin-positive osteocytes were expressed more in the control group (364.12 ± 28.12/mm<sup>2</sup>) than in the risedronate group (106.93 ± 12.85/mm<sup>2</sup>). Western blotting revealed that the expressions of RANKL, RANK, sclerostin, and BMP2 were higher in the control group than in the risedronate group (<i>p</i> < 0.05). Furthermore, RANK, sclerostin, and OPG protein levels were higher in the control group than in the risedronate group. <i>Conclusions</i>: In this study, the risedronate group demonstrated lower osteoclast activity and sclerostin expression in osteocytes in the femoral head than the control group.https://www.mdpi.com/1648-9144/58/11/1566femoral headhip fractureosteoclastrisedronatesclerostin |
| spellingShingle | Hwan-Hee Lee Eun-Yong Choi Hyun-Sik Jun Young-Yul Kim Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study Medicina femoral head hip fracture osteoclast risedronate sclerostin |
| title | Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study |
| title_full | Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study |
| title_fullStr | Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study |
| title_full_unstemmed | Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study |
| title_short | Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study |
| title_sort | osteoclast and sclerostin expression in osteocytes in the femoral head with risedronate therapy in patients with hip fractures a retrospective comparative study |
| topic | femoral head hip fracture osteoclast risedronate sclerostin |
| url | https://www.mdpi.com/1648-9144/58/11/1566 |
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