High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency
Predominantly antibody deficiencies are a category of primary immunodeficiency diseases, which consist of several rare disorders such as common variable immunodeficiency (CVID) and X-linked agammaglobulinemia (XLA). We evaluated the effects of CVID and XLA patients’ sera as a source of microenvirome...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2007-06-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/166 |
| _version_ | 1818537630250303488 |
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| author | Maryam Nourizadeh Asghar Aghamohammadi Seyed Mohammad Moazzeni Mahdi Mahdavi Nima Rezaei Jamshid Hadjati |
| author_facet | Maryam Nourizadeh Asghar Aghamohammadi Seyed Mohammad Moazzeni Mahdi Mahdavi Nima Rezaei Jamshid Hadjati |
| author_sort | Maryam Nourizadeh |
| collection | DOAJ |
| description | Predominantly antibody deficiencies are a category of primary immunodeficiency diseases, which
consist of several rare disorders such as common variable immunodeficiency (CVID) and X-linked agammaglobulinemia (XLA). We evaluated the effects of CVID and XLA patients’ sera as a source of microenviromental factors on maturation and function of monocyte-derived DCs.
Blood was collected from 10 CVID and 5 XLA patients before immunoglobulin replacement
therapy and also from 8 healthy volunteers in order to obtain necessary sera for this study. Monocyte derived DCs were generated from blood cells obtained from healthy volunteers in the presence of GM-CSF, IL-4 and 10% serum concentrations from cases and controls. Immature DCs were incubated with monocyte conditioned medium (MCM) and TNF-α in order to generate mature DCs. Interleukin 18 (IL-18) production by CD40L-activated mature DCs was measured after 24 hours of culture in vitro.
IL-18 production by DCs generated in the presence of CVID and XLA patients’ sera were
6.75±2.59 and 7.08±1.75 ng/ml, respectively, which were significantly higher than normal serum
conditioned DCs (3.55±0.68) ng/ml.
These results suggest that the sera of patients with predominantly antibody deficiencies may
contain soluble factor(s) that can induce a significant increase in IL-18 production by DCs. |
| first_indexed | 2024-12-11T18:53:14Z |
| format | Article |
| id | doaj.art-7af6535c1e9c4e8da9a46cc474e868a9 |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-11T18:53:14Z |
| publishDate | 2007-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-7af6535c1e9c4e8da9a46cc474e868a92022-12-22T00:54:13ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492007-06-0162166High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency Maryam Nourizadeh0 Asghar Aghamohammadi1 Seyed Mohammad Moazzeni2 Mahdi Mahdavi3 Nima Rezaei4 Jamshid Hadjati5 Predominantly antibody deficiencies are a category of primary immunodeficiency diseases, which consist of several rare disorders such as common variable immunodeficiency (CVID) and X-linked agammaglobulinemia (XLA). We evaluated the effects of CVID and XLA patients’ sera as a source of microenviromental factors on maturation and function of monocyte-derived DCs. Blood was collected from 10 CVID and 5 XLA patients before immunoglobulin replacement therapy and also from 8 healthy volunteers in order to obtain necessary sera for this study. Monocyte derived DCs were generated from blood cells obtained from healthy volunteers in the presence of GM-CSF, IL-4 and 10% serum concentrations from cases and controls. Immature DCs were incubated with monocyte conditioned medium (MCM) and TNF-α in order to generate mature DCs. Interleukin 18 (IL-18) production by CD40L-activated mature DCs was measured after 24 hours of culture in vitro. IL-18 production by DCs generated in the presence of CVID and XLA patients’ sera were 6.75±2.59 and 7.08±1.75 ng/ml, respectively, which were significantly higher than normal serum conditioned DCs (3.55±0.68) ng/ml. These results suggest that the sera of patients with predominantly antibody deficiencies may contain soluble factor(s) that can induce a significant increase in IL-18 production by DCs.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/166"Common variable immunodeficiencyInterleukin-18X-linked agammaglobulinemia " |
| spellingShingle | Maryam Nourizadeh Asghar Aghamohammadi Seyed Mohammad Moazzeni Mahdi Mahdavi Nima Rezaei Jamshid Hadjati High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency Iranian Journal of Allergy, Asthma and Immunology "Common variable immunodeficiency Interleukin-18 X-linked agammaglobulinemia " |
| title | High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency |
| title_full | High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency |
| title_fullStr | High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency |
| title_full_unstemmed | High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency |
| title_short | High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency |
| title_sort | high production of il 18 by dendritic cells induced by sera from patients with primary antibody deficiency |
| topic | "Common variable immunodeficiency Interleukin-18 X-linked agammaglobulinemia " |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/166 |
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