A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish
Reactive oxygen species (ROS) are significant active species in living organisms, and their coordination maintains the function of organelles to resist the invasion of foreign substances. Hypochlorous acid (HClO) is not only an eventful signaling species but also a kind of ROS, which plays an irrepl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.1009186/full |
| _version_ | 1811183730015862784 |
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| author | Liangliang Li Xiaofeng Wang Jinzhi Huang Kaidong Ma Xiaoyu Tan |
| author_facet | Liangliang Li Xiaofeng Wang Jinzhi Huang Kaidong Ma Xiaoyu Tan |
| author_sort | Liangliang Li |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) are significant active species in living organisms, and their coordination maintains the function of organelles to resist the invasion of foreign substances. Hypochlorous acid (HClO) is not only an eventful signaling species but also a kind of ROS, which plays an irreplaceable role in the immune system. However, its abnormal levels can cause cell damage or even apoptosis, which in turn leads to the onset of a series of diseases such as inflammation, neurological diseases, and even cancer. Based on this, we designed a near-infrared fluorescent probe with a large Stokes shift for ultrafast response to HClO. Furthermore, the probe exhibits excellent sensitivity and selectivity toward HClO over other species. The probe was successfully applied to visualize endogenous and exogenous HClO in living cells and in zebrafish. This unique study is the key to providing a trustworthy tool for imaging based on the in vitro and in vivo imaging of endogenous HClO, which possesses great potential for the use in future studies of HClO-related biology and pathology. |
| first_indexed | 2024-04-11T09:51:52Z |
| format | Article |
| id | doaj.art-7afa44e9f5c24ef0b198a484d6db4539 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-11T09:51:52Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-7afa44e9f5c24ef0b198a484d6db45392022-12-22T04:30:47ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-09-011010.3389/fchem.2022.10091861009186A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafishLiangliang Li0Xiaofeng Wang1Jinzhi Huang2Kaidong Ma3Xiaoyu Tan4Shenzhen Longhua District Central Hospital, Guangzhou, ChinaDepartment of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaShunde Women and Children’s Hospital of Guangdong Medical University, Foshan, Guangdong, ChinaShenzhen Longhua District Central Hospital, Guangzhou, ChinaShenzhen Longgang District Maternal and Child Health Hospital, Guangzhou, ChinaReactive oxygen species (ROS) are significant active species in living organisms, and their coordination maintains the function of organelles to resist the invasion of foreign substances. Hypochlorous acid (HClO) is not only an eventful signaling species but also a kind of ROS, which plays an irreplaceable role in the immune system. However, its abnormal levels can cause cell damage or even apoptosis, which in turn leads to the onset of a series of diseases such as inflammation, neurological diseases, and even cancer. Based on this, we designed a near-infrared fluorescent probe with a large Stokes shift for ultrafast response to HClO. Furthermore, the probe exhibits excellent sensitivity and selectivity toward HClO over other species. The probe was successfully applied to visualize endogenous and exogenous HClO in living cells and in zebrafish. This unique study is the key to providing a trustworthy tool for imaging based on the in vitro and in vivo imaging of endogenous HClO, which possesses great potential for the use in future studies of HClO-related biology and pathology.https://www.frontiersin.org/articles/10.3389/fchem.2022.1009186/fullfluorescent probeHClOnear-infraredliving cellszebrafish |
| spellingShingle | Liangliang Li Xiaofeng Wang Jinzhi Huang Kaidong Ma Xiaoyu Tan A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish Frontiers in Chemistry fluorescent probe HClO near-infrared living cells zebrafish |
| title | A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish |
| title_full | A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish |
| title_fullStr | A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish |
| title_full_unstemmed | A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish |
| title_short | A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish |
| title_sort | novel near infrared fluorescent probe for rapid sensing of hclo in living cells and zebrafish |
| topic | fluorescent probe HClO near-infrared living cells zebrafish |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.1009186/full |
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