The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet
Millet color is an important index for consumers to assess foxtail millet quality. The yellow color of millet is mainly because of the accumulation of carotenoids, which are essential for human nutrition. However, the discoloration of millet during storage due to carotenoid degradation seriously red...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/8/1283 |
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| author | Qi Ma Junjie Wang Lu Cheng Yaqiong Li Qianxiang Zhang Hongying Li Yuanhuai Han Xiaoxi Zhen Bin Zhang |
| author_facet | Qi Ma Junjie Wang Lu Cheng Yaqiong Li Qianxiang Zhang Hongying Li Yuanhuai Han Xiaoxi Zhen Bin Zhang |
| author_sort | Qi Ma |
| collection | DOAJ |
| description | Millet color is an important index for consumers to assess foxtail millet quality. The yellow color of millet is mainly because of the accumulation of carotenoids, which are essential for human nutrition. However, the discoloration of millet during storage due to carotenoid degradation seriously reduces the nutritional and commercial value of millet products. The essential quality traits of millet discoloration during storage were analyzed using two foxtail millet varieties, namely 9806-1 and Baomihunzi. We observed that the millet discoloration was caused by carotenoid degradation during storage, and different genotypes exhibited different discoloration rates. The carotenoid reduction rate in 9806-1 (32.2%) was higher than that in Baomihunzi (10.5%). The positive correlation between carotenoid reduction and the expression of SiLOX protein indicated that SiLOX from foxtail millet played a major role in carotenoid reduction during storage. The expression profiles of the <i>SiLOX</i> gene family were analyzed at different grain maturing stages, from S1 to S3, in these two varieties to determine the key <i>SiLOX</i> genes responsive to millet discoloration in foxtail millet. The consecutively low expression of <i>SiLOX2</i>, <i>SiLOX3</i>, and <i>SiLOX4</i> contributed to the low level of SiLOX protein in Baomihunzi. Furthermore, the undetectable expression of <i>SiLOX4</i> in the later stage of maturation in Baomihunzi was associated with low discoloration, indicating that <i>SiLOX4</i> might be a key gene in regulating the discoloration of millet. This study provided critical information on the mechanism of carotenoid degradation during millet storage and laid the foundation for further understanding of carotenoid metabolism in foxtail millet. |
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| language | English |
| last_indexed | 2024-03-09T10:03:31Z |
| publishDate | 2022-08-01 |
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| series | Agriculture |
| spelling | doaj.art-7afbb208e9c4459ab4bd2e605e536aae2023-12-01T23:17:14ZengMDPI AGAgriculture2077-04722022-08-01128128310.3390/agriculture12081283The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail MilletQi Ma0Junjie Wang1Lu Cheng2Yaqiong Li3Qianxiang Zhang4Hongying Li5Yuanhuai Han6Xiaoxi Zhen7Bin Zhang8College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaShanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaMillet color is an important index for consumers to assess foxtail millet quality. The yellow color of millet is mainly because of the accumulation of carotenoids, which are essential for human nutrition. However, the discoloration of millet during storage due to carotenoid degradation seriously reduces the nutritional and commercial value of millet products. The essential quality traits of millet discoloration during storage were analyzed using two foxtail millet varieties, namely 9806-1 and Baomihunzi. We observed that the millet discoloration was caused by carotenoid degradation during storage, and different genotypes exhibited different discoloration rates. The carotenoid reduction rate in 9806-1 (32.2%) was higher than that in Baomihunzi (10.5%). The positive correlation between carotenoid reduction and the expression of SiLOX protein indicated that SiLOX from foxtail millet played a major role in carotenoid reduction during storage. The expression profiles of the <i>SiLOX</i> gene family were analyzed at different grain maturing stages, from S1 to S3, in these two varieties to determine the key <i>SiLOX</i> genes responsive to millet discoloration in foxtail millet. The consecutively low expression of <i>SiLOX2</i>, <i>SiLOX3</i>, and <i>SiLOX4</i> contributed to the low level of SiLOX protein in Baomihunzi. Furthermore, the undetectable expression of <i>SiLOX4</i> in the later stage of maturation in Baomihunzi was associated with low discoloration, indicating that <i>SiLOX4</i> might be a key gene in regulating the discoloration of millet. This study provided critical information on the mechanism of carotenoid degradation during millet storage and laid the foundation for further understanding of carotenoid metabolism in foxtail millet.https://www.mdpi.com/2077-0472/12/8/1283foxtail milletlipoxygenasecarotenoiddiscoloration |
| spellingShingle | Qi Ma Junjie Wang Lu Cheng Yaqiong Li Qianxiang Zhang Hongying Li Yuanhuai Han Xiaoxi Zhen Bin Zhang The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet Agriculture foxtail millet lipoxygenase carotenoid discoloration |
| title | The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet |
| title_full | The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet |
| title_fullStr | The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet |
| title_full_unstemmed | The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet |
| title_short | The Potential Function of <i>SiLOX4</i> on Millet Discoloration during Storage in Foxtail Millet |
| title_sort | potential function of i silox4 i on millet discoloration during storage in foxtail millet |
| topic | foxtail millet lipoxygenase carotenoid discoloration |
| url | https://www.mdpi.com/2077-0472/12/8/1283 |
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