TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner
The influence of an inflammatory microenvironment on tumorigenesis has been widely accepted. Systemic conditions that favor the onset of an inflammatory landscape predispose the progression of breast cancer. Under obesity conditions, the endocrine function of adipose tissue is one of the main determ...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/4/813 |
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| author | María Jesús Vera Francisco Guajardo Felix A. Urra Nicolás Tobar Jorge Martínez |
| author_facet | María Jesús Vera Francisco Guajardo Felix A. Urra Nicolás Tobar Jorge Martínez |
| author_sort | María Jesús Vera |
| collection | DOAJ |
| description | The influence of an inflammatory microenvironment on tumorigenesis has been widely accepted. Systemic conditions that favor the onset of an inflammatory landscape predispose the progression of breast cancer. Under obesity conditions, the endocrine function of adipose tissue is one of the main determinants of the production of local and systemic inflammatory mediators. Although these mediators can stimulate tumorigenesis and recruit inflammatory cells, as macrophages, the mechanism involved remains poorly understood. In the present work, we describe that the TNFα treatment of mammary preadipocytes from human normal patients blocks adipose differentiation and promotes the generation of pro-inflammatory soluble factors. The latter stimulate the mobilization of THP-1 monocytes and MCF-7 epithelial cancer cells in an MCP1/CCL2- and mitochondrial-ROS-dependent manner. Together, these results reaffirm the contribution of an inflammatory microenvironment and mtROS in the progression of breast cancer. |
| first_indexed | 2024-03-11T05:18:02Z |
| format | Article |
| id | doaj.art-7afd3d09eb9e4c5195944104f420fd46 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T05:18:02Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7afd3d09eb9e4c5195944104f420fd462023-11-17T18:04:40ZengMDPI AGAntioxidants2076-39212023-03-0112481310.3390/antiox12040813TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent MannerMaría Jesús Vera0Francisco Guajardo1Felix A. Urra2Nicolás Tobar3Jorge Martínez4Molecular and Cell Biology Laboratory, INTA, University of Chile, Santiago 7830490, ChileMIBI: Interdisciplinary Group on Mitochondrial Targeting and Bioenergetics, Santiago 8380453, ChileMIBI: Interdisciplinary Group on Mitochondrial Targeting and Bioenergetics, Santiago 8380453, ChileMolecular and Cell Biology Laboratory, INTA, University of Chile, Santiago 7830490, ChileMolecular and Cell Biology Laboratory, INTA, University of Chile, Santiago 7830490, ChileThe influence of an inflammatory microenvironment on tumorigenesis has been widely accepted. Systemic conditions that favor the onset of an inflammatory landscape predispose the progression of breast cancer. Under obesity conditions, the endocrine function of adipose tissue is one of the main determinants of the production of local and systemic inflammatory mediators. Although these mediators can stimulate tumorigenesis and recruit inflammatory cells, as macrophages, the mechanism involved remains poorly understood. In the present work, we describe that the TNFα treatment of mammary preadipocytes from human normal patients blocks adipose differentiation and promotes the generation of pro-inflammatory soluble factors. The latter stimulate the mobilization of THP-1 monocytes and MCF-7 epithelial cancer cells in an MCP1/CCL2- and mitochondrial-ROS-dependent manner. Together, these results reaffirm the contribution of an inflammatory microenvironment and mtROS in the progression of breast cancer.https://www.mdpi.com/2076-3921/12/4/813migrationmonocytesbreast cancerOXPHOSadipocytes |
| spellingShingle | María Jesús Vera Francisco Guajardo Felix A. Urra Nicolás Tobar Jorge Martínez TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner Antioxidants migration monocytes breast cancer OXPHOS adipocytes |
| title | TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner |
| title_full | TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner |
| title_fullStr | TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner |
| title_full_unstemmed | TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner |
| title_short | TNF-Alpha Promotes an Inflammatory Mammary Microenvironment That Favors Macrophage and Epithelial Migration in a CCL2- and Mitochondrial-ROS-Dependent Manner |
| title_sort | tnf alpha promotes an inflammatory mammary microenvironment that favors macrophage and epithelial migration in a ccl2 and mitochondrial ros dependent manner |
| topic | migration monocytes breast cancer OXPHOS adipocytes |
| url | https://www.mdpi.com/2076-3921/12/4/813 |
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