Influence of human and bovine substrate on the microleakage of two adhesive systems
The aim of this study was to evaluate in vitro the marginal sealing of two adhesive systems and to analyze the influence of human and bovine substrates on marginal microleakage in enamel. Rectangular-shaped class V cavities (4 mm wide x 2 mm high x 2 mm deep) were made as follows: 8 cavities were pr...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2009-04-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004 |
| _version_ | 1818602626422407168 |
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| author | Karoline Guará Brusaca Almeida Kristine Guará Brusaca Almeida Scheibe Ana Emília Figuerêdo Oliveira Cláudia Maria Coêlho Alves José Ferreira Costa |
| author_facet | Karoline Guará Brusaca Almeida Kristine Guará Brusaca Almeida Scheibe Ana Emília Figuerêdo Oliveira Cláudia Maria Coêlho Alves José Ferreira Costa |
| author_sort | Karoline Guará Brusaca Almeida |
| collection | DOAJ |
| description | The aim of this study was to evaluate in vitro the marginal sealing of two adhesive systems and to analyze the influence of human and bovine substrates on marginal microleakage in enamel. Rectangular-shaped class V cavities (4 mm wide x 2 mm high x 2 mm deep) were made as follows: 8 cavities were prepared on the buccal and lingual surfaces of the human teeth with margins located on enamel and 16 cavities were prepared on the buccal surfaces of the bovine teeth. The cavities were randomly assigned to 4 groups of 8 cavities according to the adhesive system and substrate: G1 - Prime & Bond 2.1 (Dentsply)/human substrate; G2 - Adhese (Ivoclar/Vivadent)/human substrate; G3 - Prime & Bond 2.1 (Dentsply)/bovine substrate; G4 - Adhese (Ivoclar/Vivadent)/bovine substrate. The cavities were filled with microhybrid composite resin (Fillmagic) and after polishing/finishing procedures, the teeth were subjected to a thermocycling regimen of 500 cycles with 1-min immersions in water at 55° ±2°C and 5° ± 2°C. Next, the teeth were coated with two layers of nail polish to within 1 mm of the margin, submerged in a 50% silver nitrate solution for 2 h, rinsed thoroughly in running tap and immersed in developing solution for 8 h. The restorations were bisected resulting in 16 specimens. Microleakage was observed under a stereomicroscope at x25 and recorded using four-point (0-3) scoring system. The data were analyzed statistically by the Mann Whitney U-test at 5% significance level. Leakage was present in all specimens and there was statistically significant difference between the adhesive systems. Adhese self-etching system showed significantly more leakage in both substrates (human - p= 0.0001 and bovine - p= 0.0031). There was no statistically significant difference between human and bovine substrates for either of the adhesive systems based on different bonding mechanisms (Prime & Bond 2.1 - p= 0.6923 and Adhese - p= 0.6109). Neither of the adhesive systems was capable to completely prevent microleakage and the self-etching system was more susceptible to microleakage. |
| first_indexed | 2024-12-16T13:10:16Z |
| format | Article |
| id | doaj.art-7b00a2211f924342affa1195fabb0299 |
| institution | Directory Open Access Journal |
| issn | 1678-7757 1678-7765 |
| language | English |
| last_indexed | 2024-12-16T13:10:16Z |
| publishDate | 2009-04-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-7b00a2211f924342affa1195fabb02992022-12-21T22:30:38ZengUniversity of São PauloJournal of Applied Oral Science1678-77571678-77652009-04-01172929610.1590/S1678-77572009000200004Influence of human and bovine substrate on the microleakage of two adhesive systemsKaroline Guará Brusaca AlmeidaKristine Guará Brusaca Almeida ScheibeAna Emília Figuerêdo OliveiraCláudia Maria Coêlho AlvesJosé Ferreira CostaThe aim of this study was to evaluate in vitro the marginal sealing of two adhesive systems and to analyze the influence of human and bovine substrates on marginal microleakage in enamel. Rectangular-shaped class V cavities (4 mm wide x 2 mm high x 2 mm deep) were made as follows: 8 cavities were prepared on the buccal and lingual surfaces of the human teeth with margins located on enamel and 16 cavities were prepared on the buccal surfaces of the bovine teeth. The cavities were randomly assigned to 4 groups of 8 cavities according to the adhesive system and substrate: G1 - Prime & Bond 2.1 (Dentsply)/human substrate; G2 - Adhese (Ivoclar/Vivadent)/human substrate; G3 - Prime & Bond 2.1 (Dentsply)/bovine substrate; G4 - Adhese (Ivoclar/Vivadent)/bovine substrate. The cavities were filled with microhybrid composite resin (Fillmagic) and after polishing/finishing procedures, the teeth were subjected to a thermocycling regimen of 500 cycles with 1-min immersions in water at 55° ±2°C and 5° ± 2°C. Next, the teeth were coated with two layers of nail polish to within 1 mm of the margin, submerged in a 50% silver nitrate solution for 2 h, rinsed thoroughly in running tap and immersed in developing solution for 8 h. The restorations were bisected resulting in 16 specimens. Microleakage was observed under a stereomicroscope at x25 and recorded using four-point (0-3) scoring system. The data were analyzed statistically by the Mann Whitney U-test at 5% significance level. Leakage was present in all specimens and there was statistically significant difference between the adhesive systems. Adhese self-etching system showed significantly more leakage in both substrates (human - p= 0.0001 and bovine - p= 0.0031). There was no statistically significant difference between human and bovine substrates for either of the adhesive systems based on different bonding mechanisms (Prime & Bond 2.1 - p= 0.6923 and Adhese - p= 0.6109). Neither of the adhesive systems was capable to completely prevent microleakage and the self-etching system was more susceptible to microleakage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004ToothMicroleakageBovine and human teethSelf-etching adhesives |
| spellingShingle | Karoline Guará Brusaca Almeida Kristine Guará Brusaca Almeida Scheibe Ana Emília Figuerêdo Oliveira Cláudia Maria Coêlho Alves José Ferreira Costa Influence of human and bovine substrate on the microleakage of two adhesive systems Journal of Applied Oral Science Tooth Microleakage Bovine and human teeth Self-etching adhesives |
| title | Influence of human and bovine substrate on the microleakage of two adhesive systems |
| title_full | Influence of human and bovine substrate on the microleakage of two adhesive systems |
| title_fullStr | Influence of human and bovine substrate on the microleakage of two adhesive systems |
| title_full_unstemmed | Influence of human and bovine substrate on the microleakage of two adhesive systems |
| title_short | Influence of human and bovine substrate on the microleakage of two adhesive systems |
| title_sort | influence of human and bovine substrate on the microleakage of two adhesive systems |
| topic | Tooth Microleakage Bovine and human teeth Self-etching adhesives |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004 |
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