Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations
<p>Abstract</p> <p>Background</p> <p>The karyotypes of <it>Leptodactylus</it> species usually consist of 22 bi-armed chromosomes, but morphological variations in some chromosomes and even differences in the 2n have been reported. To better understand the mec...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-12-01
|
| Series: | BMC Genetics |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2156/13/109 |
| _version_ | 1811289168762896384 |
|---|---|
| author | Gazoni Thiago Gruber Simone L Silva Ana PZ Araújo Olivia GS Narimatsu Hideki Strüssmann Christine Haddad Célio FB Kasahara Sanae |
| author_facet | Gazoni Thiago Gruber Simone L Silva Ana PZ Araújo Olivia GS Narimatsu Hideki Strüssmann Christine Haddad Célio FB Kasahara Sanae |
| author_sort | Gazoni Thiago |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The karyotypes of <it>Leptodactylus</it> species usually consist of 22 bi-armed chromosomes, but morphological variations in some chromosomes and even differences in the 2n have been reported. To better understand the mechanisms responsible for these differences, eight species were analysed using classical and molecular cytogenetic techniques, including replication banding with BrdU incorporation.</p> <p>Results</p> <p>Distinct chromosome numbers were found: 2n = 22 in <it>Leptodactylus chaquensis</it>, <it>L</it>. <it>labyrinthicus</it>, <it>L</it>. <it>pentadactylus</it>, <it>L</it>. <it>petersii</it>, <it>L</it>. <it>podicipinus</it>, and <it>L</it>. <it>rhodomystax</it>; 2n = 20 in <it>Leptodactylus</it> sp<it>.</it> (aff. <it>podicipinus</it>); and 2n = 24 in <it>L</it>. <it>marmoratus</it>. Among the species with 2n = 22, only three had the same basic karyotype. <it>Leptodactylus pentadactylus</it> presented multiple translocations, <it>L</it>. <it>petersii</it> displayed chromosome morphological discrepancy, and <it>L</it>. <it>podicipinus</it> had four pairs of telocentric chromosomes. Replication banding was crucial for characterising this variability and for explaining the reduced 2n in <it>Leptodactylus</it> sp<it>.</it> (aff. <it>podicipinus</it>). <it>Leptodactylus marmoratus</it> had few chromosomes with a similar banding patterns to the 2n = 22 karyotypes. The majority of the species presented a single NOR-bearing pair, which was confirmed using Ag-impregnation and FISH with an rDNA probe. In general, the NOR-bearing chromosomes corresponded to chromosome 8, but NORs were found on chromosome 3 or 4 in some species. <it>Leptodactylus marmoratus</it> had NORs on chromosome pairs 6 and 8. The data from C-banding, fluorochrome staining, and FISH using the telomeric probe helped in characterising the repetitive sequences. Even though hybridisation did occur on the chromosome ends, telomere-like repetitive sequences outside of the telomere region were identified. Metaphase I cells from <it>L</it>. <it>pentadactylus</it> confirmed its complex karyotype constitution because 12 chromosomes appeared as ring-shaped chain in addition to five bivalents.</p> <p>Conclusions</p> <p>Species of <it>Leptodactylus</it> exhibited both major and minor karyotypic differences which were identified by classical and molecular cytogenetic techniques. Replication banding, which is a unique procedure that has been used to obtain longitudinal multiple band patterns in amphibian chromosomes, allowed us to outline the general mechanisms responsible for these karyotype differences. The findings also suggested that <it>L</it>. <it>marmoratus</it>, which was formerly included in the genus <it>Adenomera</it>, may have undergone great chromosomal repatterning.</p> |
| first_indexed | 2024-04-13T03:50:07Z |
| format | Article |
| id | doaj.art-7b032ff66b79471583035c858e21f3d0 |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-13T03:50:07Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-7b032ff66b79471583035c858e21f3d02022-12-22T03:03:50ZengBMCBMC Genetics1471-21562012-12-0113110910.1186/1471-2156-13-109Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocationsGazoni ThiagoGruber Simone LSilva Ana PZAraújo Olivia GSNarimatsu HidekiStrüssmann ChristineHaddad Célio FBKasahara Sanae<p>Abstract</p> <p>Background</p> <p>The karyotypes of <it>Leptodactylus</it> species usually consist of 22 bi-armed chromosomes, but morphological variations in some chromosomes and even differences in the 2n have been reported. To better understand the mechanisms responsible for these differences, eight species were analysed using classical and molecular cytogenetic techniques, including replication banding with BrdU incorporation.</p> <p>Results</p> <p>Distinct chromosome numbers were found: 2n = 22 in <it>Leptodactylus chaquensis</it>, <it>L</it>. <it>labyrinthicus</it>, <it>L</it>. <it>pentadactylus</it>, <it>L</it>. <it>petersii</it>, <it>L</it>. <it>podicipinus</it>, and <it>L</it>. <it>rhodomystax</it>; 2n = 20 in <it>Leptodactylus</it> sp<it>.</it> (aff. <it>podicipinus</it>); and 2n = 24 in <it>L</it>. <it>marmoratus</it>. Among the species with 2n = 22, only three had the same basic karyotype. <it>Leptodactylus pentadactylus</it> presented multiple translocations, <it>L</it>. <it>petersii</it> displayed chromosome morphological discrepancy, and <it>L</it>. <it>podicipinus</it> had four pairs of telocentric chromosomes. Replication banding was crucial for characterising this variability and for explaining the reduced 2n in <it>Leptodactylus</it> sp<it>.</it> (aff. <it>podicipinus</it>). <it>Leptodactylus marmoratus</it> had few chromosomes with a similar banding patterns to the 2n = 22 karyotypes. The majority of the species presented a single NOR-bearing pair, which was confirmed using Ag-impregnation and FISH with an rDNA probe. In general, the NOR-bearing chromosomes corresponded to chromosome 8, but NORs were found on chromosome 3 or 4 in some species. <it>Leptodactylus marmoratus</it> had NORs on chromosome pairs 6 and 8. The data from C-banding, fluorochrome staining, and FISH using the telomeric probe helped in characterising the repetitive sequences. Even though hybridisation did occur on the chromosome ends, telomere-like repetitive sequences outside of the telomere region were identified. Metaphase I cells from <it>L</it>. <it>pentadactylus</it> confirmed its complex karyotype constitution because 12 chromosomes appeared as ring-shaped chain in addition to five bivalents.</p> <p>Conclusions</p> <p>Species of <it>Leptodactylus</it> exhibited both major and minor karyotypic differences which were identified by classical and molecular cytogenetic techniques. Replication banding, which is a unique procedure that has been used to obtain longitudinal multiple band patterns in amphibian chromosomes, allowed us to outline the general mechanisms responsible for these karyotype differences. The findings also suggested that <it>L</it>. <it>marmoratus</it>, which was formerly included in the genus <it>Adenomera</it>, may have undergone great chromosomal repatterning.</p>http://www.biomedcentral.com/1471-2156/13/109FISHReplication bandingBrdUFluorochrome stainingAg-NORC-banding |
| spellingShingle | Gazoni Thiago Gruber Simone L Silva Ana PZ Araújo Olivia GS Narimatsu Hideki Strüssmann Christine Haddad Célio FB Kasahara Sanae Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations BMC Genetics FISH Replication banding BrdU Fluorochrome staining Ag-NOR C-banding |
| title | Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations |
| title_full | Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations |
| title_fullStr | Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations |
| title_full_unstemmed | Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations |
| title_short | Cytogenetic analyses of eight species in the genus <it>Leptodactylus</it> Fitzinger, 1843 (Amphibia, Anura, Leptodactylidae), including a new diploid number and a karyotype with multiple translocations |
| title_sort | cytogenetic analyses of eight species in the genus it leptodactylus it fitzinger 1843 amphibia anura leptodactylidae including a new diploid number and a karyotype with multiple translocations |
| topic | FISH Replication banding BrdU Fluorochrome staining Ag-NOR C-banding |
| url | http://www.biomedcentral.com/1471-2156/13/109 |
| work_keys_str_mv | AT gazonithiago cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT grubersimonel cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT silvaanapz cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT araujooliviags cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT narimatsuhideki cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT strussmannchristine cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT haddadceliofb cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations AT kasaharasanae cytogeneticanalysesofeightspeciesinthegenusitleptodactylusitfitzinger1843amphibiaanuraleptodactylidaeincludinganewdiploidnumberandakaryotypewithmultipletranslocations |