Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis
Environmental DNA (eDNA) is a promising tool for rapid and noninvasive biomonitoring and resource assessment. Broadly, two main PCR-based strategies of biodiversity monitoring can be deployed for eDNA analysis. The first one consists of targeting single species using standard PCR, real-time quantita...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.890756/full |
| _version_ | 1818017537255800832 |
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| author | Lisheng Wu Lisheng Wu Jianlong Li Jianlong Li Feng Tong Jiajun Zhang Miaomiao Li Shaoxiong Ding Shaoxiong Ding |
| author_facet | Lisheng Wu Lisheng Wu Jianlong Li Jianlong Li Feng Tong Jiajun Zhang Miaomiao Li Shaoxiong Ding Shaoxiong Ding |
| author_sort | Lisheng Wu |
| collection | DOAJ |
| description | Environmental DNA (eDNA) is a promising tool for rapid and noninvasive biomonitoring and resource assessment. Broadly, two main PCR-based strategies of biodiversity monitoring can be deployed for eDNA analysis. The first one consists of targeting single species using standard PCR, real-time quantitative PCR (qPCR), or droplet digital PCR (ddPCR). The second strategy aims to simultaneously detect multiple species relying on High-Throughput Sequencing (i.e. eDNA metabarcoding). The aim of this study was to further explore a convenient and feasible method to correctly assess fishery resources using eDNA technology for large yellow croaker (Larimichthys crocea). Two eDNA methods (metabarcoding and qPCR) were used to investigate the biomass and distribution of large yellow croaker in the autumn and spring seasons in the central and southern regions of the East China Sea, and these results were compared with the results of a bottom trawling fishery survey. The results suggested that the designed NADH2-specific primers and developed real-time qPCR method were more applicable and reliable. The variation in the eDNA concentration of large yellow croakers coincided well with that of a real capture survey in the East China Sea. The difference in eDNA concentration between the surface and bottom water samples reflected the distribution characteristics in the water layer related to the life history of L. crocea in the two seasons. The developed methods based on eDNA technology and real-time qPCR could not only be used to evaluate the resource distribution but could also contribute to estimating the life history and migration route of large yellow croaker. |
| first_indexed | 2024-04-14T07:28:17Z |
| format | Article |
| id | doaj.art-7b04e7b0d9354fd2808fc8d7c4beaae5 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-14T07:28:17Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-7b04e7b0d9354fd2808fc8d7c4beaae52022-12-22T02:05:56ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-04-01910.3389/fmars.2022.890756890756Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA AnalysisLisheng Wu0Lisheng Wu1Jianlong Li2Jianlong Li3Feng Tong4Jiajun Zhang5Miaomiao Li6Shaoxiong Ding7Shaoxiong Ding8State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFunction Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, ChinaDepartment of Marine Sciences, College of Marine Sciences, Hainan University, Haikou, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Provincial Fishery Technical Extension Center, Fuzhou, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFunction Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaEnvironmental DNA (eDNA) is a promising tool for rapid and noninvasive biomonitoring and resource assessment. Broadly, two main PCR-based strategies of biodiversity monitoring can be deployed for eDNA analysis. The first one consists of targeting single species using standard PCR, real-time quantitative PCR (qPCR), or droplet digital PCR (ddPCR). The second strategy aims to simultaneously detect multiple species relying on High-Throughput Sequencing (i.e. eDNA metabarcoding). The aim of this study was to further explore a convenient and feasible method to correctly assess fishery resources using eDNA technology for large yellow croaker (Larimichthys crocea). Two eDNA methods (metabarcoding and qPCR) were used to investigate the biomass and distribution of large yellow croaker in the autumn and spring seasons in the central and southern regions of the East China Sea, and these results were compared with the results of a bottom trawling fishery survey. The results suggested that the designed NADH2-specific primers and developed real-time qPCR method were more applicable and reliable. The variation in the eDNA concentration of large yellow croakers coincided well with that of a real capture survey in the East China Sea. The difference in eDNA concentration between the surface and bottom water samples reflected the distribution characteristics in the water layer related to the life history of L. crocea in the two seasons. The developed methods based on eDNA technology and real-time qPCR could not only be used to evaluate the resource distribution but could also contribute to estimating the life history and migration route of large yellow croaker.https://www.frontiersin.org/articles/10.3389/fmars.2022.890756/fulllarge yellow croakerLarimichthys croceaeDNAfisheries resource assessmentreal-time qPCRmetabarcoding |
| spellingShingle | Lisheng Wu Lisheng Wu Jianlong Li Jianlong Li Feng Tong Jiajun Zhang Miaomiao Li Shaoxiong Ding Shaoxiong Ding Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis Frontiers in Marine Science large yellow croaker Larimichthys crocea eDNA fisheries resource assessment real-time qPCR metabarcoding |
| title | Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis |
| title_full | Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis |
| title_fullStr | Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis |
| title_full_unstemmed | Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis |
| title_short | Resource Assessment of Larimichthys crocea in the East China Sea Based on eDNA Analysis |
| title_sort | resource assessment of larimichthys crocea in the east china sea based on edna analysis |
| topic | large yellow croaker Larimichthys crocea eDNA fisheries resource assessment real-time qPCR metabarcoding |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.890756/full |
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