Cell separation: Terminology and practical considerations
Cell separation is a powerful tool in biological research. Increasing usage, particularly within the tissue engineering and regenerative medicine communities, means that researchers from a diverse range of backgrounds are utilising cell separation technologies. This review aims to offer potential so...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2013-01-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731412472690 |
| _version_ | 1828445464398135296 |
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| author | Matthew J Tomlinson Sophie Tomlinson Xuebin B Yang Jennifer Kirkham |
| author_facet | Matthew J Tomlinson Sophie Tomlinson Xuebin B Yang Jennifer Kirkham |
| author_sort | Matthew J Tomlinson |
| collection | DOAJ |
| description | Cell separation is a powerful tool in biological research. Increasing usage, particularly within the tissue engineering and regenerative medicine communities, means that researchers from a diverse range of backgrounds are utilising cell separation technologies. This review aims to offer potential solutions to cell sorting problems and to clarify common ambiguities in terminology and experimental design. The frequently used cell separation terms of ‘purity’, ‘recovery’ and ‘viability’ are discussed, and attempts are made to reach a consensus view of their sometimes ambiguous meanings. The importance of appropriate experimental design is considered, with aspects such as marker expression, tissue isolation and original cell population analysis discussed. Finally, specific technical issues such as cell clustering, dead cell removal and non-specific antibody binding are considered and potential solutions offered. The solutions offered may provide a starting point to improve the quality of cell separations achieved by both the novice and experienced researcher alike. |
| first_indexed | 2024-12-10T21:58:29Z |
| format | Article |
| id | doaj.art-7b05e3a2abad4f6b971e36d24361c696 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-10T21:58:29Z |
| publishDate | 2013-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-7b05e3a2abad4f6b971e36d24361c6962022-12-22T01:31:59ZengSAGE PublishingJournal of Tissue Engineering2041-73142013-01-01410.1177/204173141247269010.1177_2041731412472690Cell separation: Terminology and practical considerationsMatthew J Tomlinson0Sophie Tomlinson1Xuebin B Yang2Jennifer Kirkham3Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UKDepartment of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UKNIHR Leeds Musculoskeletal Biomedical Research Unit, Chapel Allerton Hospital, Leeds, UKNIHR Leeds Musculoskeletal Biomedical Research Unit, Chapel Allerton Hospital, Leeds, UKCell separation is a powerful tool in biological research. Increasing usage, particularly within the tissue engineering and regenerative medicine communities, means that researchers from a diverse range of backgrounds are utilising cell separation technologies. This review aims to offer potential solutions to cell sorting problems and to clarify common ambiguities in terminology and experimental design. The frequently used cell separation terms of ‘purity’, ‘recovery’ and ‘viability’ are discussed, and attempts are made to reach a consensus view of their sometimes ambiguous meanings. The importance of appropriate experimental design is considered, with aspects such as marker expression, tissue isolation and original cell population analysis discussed. Finally, specific technical issues such as cell clustering, dead cell removal and non-specific antibody binding are considered and potential solutions offered. The solutions offered may provide a starting point to improve the quality of cell separations achieved by both the novice and experienced researcher alike.https://doi.org/10.1177/2041731412472690 |
| spellingShingle | Matthew J Tomlinson Sophie Tomlinson Xuebin B Yang Jennifer Kirkham Cell separation: Terminology and practical considerations Journal of Tissue Engineering |
| title | Cell separation: Terminology and practical considerations |
| title_full | Cell separation: Terminology and practical considerations |
| title_fullStr | Cell separation: Terminology and practical considerations |
| title_full_unstemmed | Cell separation: Terminology and practical considerations |
| title_short | Cell separation: Terminology and practical considerations |
| title_sort | cell separation terminology and practical considerations |
| url | https://doi.org/10.1177/2041731412472690 |
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