The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline
Histone H3 Lys 4 methylation (H3K4me) is deposited by the conserved SET1/MLL methyltransferases acting in multiprotein complexes, including Ash2 and Wdr5. Although individual subunits contribute to complex activity, how they influence gene expression in specific tissues remains largely unknown. In C...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-10-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714007864 |
| _version_ | 1819034175904153600 |
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| author | Valérie J. Robert Marine G. Mercier Cécile Bedet Stéphane Janczarski Jorge Merlet Steve Garvis Rafal Ciosk Francesca Palladino |
| author_facet | Valérie J. Robert Marine G. Mercier Cécile Bedet Stéphane Janczarski Jorge Merlet Steve Garvis Rafal Ciosk Francesca Palladino |
| author_sort | Valérie J. Robert |
| collection | DOAJ |
| description | Histone H3 Lys 4 methylation (H3K4me) is deposited by the conserved SET1/MLL methyltransferases acting in multiprotein complexes, including Ash2 and Wdr5. Although individual subunits contribute to complex activity, how they influence gene expression in specific tissues remains largely unknown. In Caenorhabditis elegans, SET-2/SET1, WDR-5.1, and ASH-2 are differentially required for germline H3K4 methylation. Using expression profiling on germlines from animals lacking set-2, ash-2, or wdr-5.1, we show that these subunits play unique as well as redundant functions in order to promote expression of germline genes and repress somatic genes. Furthermore, we show that in set-2- and wdr-5.1-deficient germlines, somatic gene misexpression is associated with conversion of germ cells into somatic cells and that nuclear RNAi acts in parallel with SET-2 and WDR-5.1 to maintain germline identity. These findings uncover a unique role for SET-2 and WDR-5.1 in preserving germline pluripotency and underline the complexity of the cellular network regulating this process. |
| first_indexed | 2024-12-21T07:29:34Z |
| format | Article |
| id | doaj.art-7b0845342d244d3b8c4f4273e09a107d |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T07:29:34Z |
| publishDate | 2014-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-7b0845342d244d3b8c4f4273e09a107d2022-12-21T19:11:36ZengElsevierCell Reports2211-12472014-10-019244345010.1016/j.celrep.2014.09.018The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans GermlineValérie J. Robert0Marine G. Mercier1Cécile Bedet2Stéphane Janczarski3Jorge Merlet4Steve Garvis5Rafal Ciosk6Francesca Palladino7Laboratory of Molecular and Cellular Biology, CNRS, Université de Lyon 1, Ecole Normale Supérieure, 69364 Lyon Cedex 07, FranceLaboratory of Molecular and Cellular Biology, CNRS, Université de Lyon 1, Ecole Normale Supérieure, 69364 Lyon Cedex 07, FranceLaboratory of Molecular and Cellular Biology, CNRS, Université de Lyon 1, Ecole Normale Supérieure, 69364 Lyon Cedex 07, FranceLaboratory of Molecular and Cellular Biology, CNRS, Université de Lyon 1, Ecole Normale Supérieure, 69364 Lyon Cedex 07, FranceFriedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, SwitzerlandLaboratory of Molecular and Cellular Biology, CNRS, Université de Lyon 1, Ecole Normale Supérieure, 69364 Lyon Cedex 07, FranceFriedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, SwitzerlandLaboratory of Molecular and Cellular Biology, CNRS, Université de Lyon 1, Ecole Normale Supérieure, 69364 Lyon Cedex 07, FranceHistone H3 Lys 4 methylation (H3K4me) is deposited by the conserved SET1/MLL methyltransferases acting in multiprotein complexes, including Ash2 and Wdr5. Although individual subunits contribute to complex activity, how they influence gene expression in specific tissues remains largely unknown. In Caenorhabditis elegans, SET-2/SET1, WDR-5.1, and ASH-2 are differentially required for germline H3K4 methylation. Using expression profiling on germlines from animals lacking set-2, ash-2, or wdr-5.1, we show that these subunits play unique as well as redundant functions in order to promote expression of germline genes and repress somatic genes. Furthermore, we show that in set-2- and wdr-5.1-deficient germlines, somatic gene misexpression is associated with conversion of germ cells into somatic cells and that nuclear RNAi acts in parallel with SET-2 and WDR-5.1 to maintain germline identity. These findings uncover a unique role for SET-2 and WDR-5.1 in preserving germline pluripotency and underline the complexity of the cellular network regulating this process.http://www.sciencedirect.com/science/article/pii/S2211124714007864 |
| spellingShingle | Valérie J. Robert Marine G. Mercier Cécile Bedet Stéphane Janczarski Jorge Merlet Steve Garvis Rafal Ciosk Francesca Palladino The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline Cell Reports |
| title | The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline |
| title_full | The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline |
| title_fullStr | The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline |
| title_full_unstemmed | The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline |
| title_short | The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline |
| title_sort | set 2 set1 histone h3k4 methyltransferase maintains pluripotency in the caenorhabditis elegans germline |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714007864 |
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