A histopathological exploration of the Madurella mycetomatis grain.
Although the Madurella mycetomatis grains seem to interfere with the host defense mechanisms and impede the antifungal drugs penetration, yet their histological features are not fully known and hence this study was set out to determine that. The study included 80 patients with confirmed M. mycetomat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483927/?tool=EBI |
| _version_ | 1818573661309763584 |
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| author | Anahid Izzat Ibrahim Ahmed Mohammed El Hassan Ahmed Fahal Wendy W van de Sande |
| author_facet | Anahid Izzat Ibrahim Ahmed Mohammed El Hassan Ahmed Fahal Wendy W van de Sande |
| author_sort | Anahid Izzat Ibrahim |
| collection | DOAJ |
| description | Although the Madurella mycetomatis grains seem to interfere with the host defense mechanisms and impede the antifungal drugs penetration, yet their histological features are not fully known and hence this study was set out to determine that. The study included 80 patients with confirmed M. mycetomatis eumycetoma. After informed written consent, surgical biopsies were obtained from the excised tissues during the patients' surgical treatment. All sections were stained with haematoxylin and eosin, Grocott's hexamine silver, Periodic Acid-Schiff's, Masson-Fontana, Perl's Prussian Blue, Von-kossa's, Formalin Inducing Fluorescence and Schmorl's stains. Modified bleaching technique was used. The concentrations of Zinc, Copper, Calcium, Iron, Lead, Cobalt and Nickel were determined by Atomic Absorption Spectrophotometer. The M. Mycetomatis grains appeared to consist of lipid, protein and melanin. The melanin was located on the hyphal wall as thick layers. The Zinc, Copper and Calcium concentrations in the grains were four, six, and sixteen folds higher than in normal tissue respectively, the other metals were found in the same concentrations as in normal tissue. In the grains, calcium was located in the melanin vicinity. From this study, it can be concluded that, the grains contain melanin, heavy metals, proteins, lipids and they contribute in the formation of the grain cement matrix. These elements seem to contribute in the organism pathogenicity and might impede the penetration of various anti-fungal agents. |
| first_indexed | 2024-12-15T00:14:29Z |
| format | Article |
| id | doaj.art-7b0927feda774a38bc302d2ba1ad29d7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-15T00:14:29Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7b0927feda774a38bc302d2ba1ad29d72022-12-21T22:42:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5777410.1371/journal.pone.0057774A histopathological exploration of the Madurella mycetomatis grain.Anahid Izzat IbrahimAhmed Mohammed El HassanAhmed FahalWendy W van de SandeAlthough the Madurella mycetomatis grains seem to interfere with the host defense mechanisms and impede the antifungal drugs penetration, yet their histological features are not fully known and hence this study was set out to determine that. The study included 80 patients with confirmed M. mycetomatis eumycetoma. After informed written consent, surgical biopsies were obtained from the excised tissues during the patients' surgical treatment. All sections were stained with haematoxylin and eosin, Grocott's hexamine silver, Periodic Acid-Schiff's, Masson-Fontana, Perl's Prussian Blue, Von-kossa's, Formalin Inducing Fluorescence and Schmorl's stains. Modified bleaching technique was used. The concentrations of Zinc, Copper, Calcium, Iron, Lead, Cobalt and Nickel were determined by Atomic Absorption Spectrophotometer. The M. Mycetomatis grains appeared to consist of lipid, protein and melanin. The melanin was located on the hyphal wall as thick layers. The Zinc, Copper and Calcium concentrations in the grains were four, six, and sixteen folds higher than in normal tissue respectively, the other metals were found in the same concentrations as in normal tissue. In the grains, calcium was located in the melanin vicinity. From this study, it can be concluded that, the grains contain melanin, heavy metals, proteins, lipids and they contribute in the formation of the grain cement matrix. These elements seem to contribute in the organism pathogenicity and might impede the penetration of various anti-fungal agents.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483927/?tool=EBI |
| spellingShingle | Anahid Izzat Ibrahim Ahmed Mohammed El Hassan Ahmed Fahal Wendy W van de Sande A histopathological exploration of the Madurella mycetomatis grain. PLoS ONE |
| title | A histopathological exploration of the Madurella mycetomatis grain. |
| title_full | A histopathological exploration of the Madurella mycetomatis grain. |
| title_fullStr | A histopathological exploration of the Madurella mycetomatis grain. |
| title_full_unstemmed | A histopathological exploration of the Madurella mycetomatis grain. |
| title_short | A histopathological exploration of the Madurella mycetomatis grain. |
| title_sort | histopathological exploration of the madurella mycetomatis grain |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483927/?tool=EBI |
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