Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens
Poultry, mainly chickens, and their white meat represent one of the main, nutritionally valuable, and affordable red meat replacer sources of protein throughout the whole world with special reference to developing countries. A long list of microbial agents especially bacterial pathogens threatens c...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-06-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1320 |
| _version_ | 1797657055601885184 |
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| author | Sally Roshdy Rafik Soliman Manal Aly Lamiaa Omar Ahmed Sameer Heidy Abo-Elyazeed Hassan Aboul-Ella |
| author_facet | Sally Roshdy Rafik Soliman Manal Aly Lamiaa Omar Ahmed Sameer Heidy Abo-Elyazeed Hassan Aboul-Ella |
| author_sort | Sally Roshdy |
| collection | DOAJ |
| description |
Poultry, mainly chickens, and their white meat represent one of the main, nutritionally valuable, and affordable red meat replacer sources of protein throughout the whole world with special reference to developing countries. A long list of microbial agents especially bacterial pathogens threatens chickens’ production cycles. They constitute one of the major problems facing the rapidly expanding poultry industry and are responsible for considerable economic losses. Fowl cholera, infectious coryza, and ornithobacteriosis (ORT) were among the serious bacterial infections that affect the respiratory tract of chickens with a global adverse effect on poultry production. A formalinized whole culture vaccine composed of Pasteurella multocida serotypes A5, A8, A9, and D2, Avibacterium paragallinarum serotypes A and C, and Ornithobacterium rhinotracheale serotype A was prepared. This polyvalent vaccine proved to be safe producing no adverse side effects when injected in chickens. The immunizing efficacy of this vaccine was evaluated in specific pathogen-free (SPF) chickens, which were immunized at 6 weeks of age. The protective efficacy of the vaccine was determined using a challenge test. The developed vaccine was effective in protecting chickens against fowl cholera, infectious coryza, and ornithobacteriosis in chickens against challenge with these pathogens. Vaccinated chickens challenged with virulent Pasteurella multocida serotypes A5, A8, A9, and D2 showed protection rates of 86.6%, 93.3%, 93.3%, and 93.3%, respectively, as compared with 100% mortality in the non-vaccinated control. Vaccinated chickens challenged with Avibacterium paragallinarum serotypes A and C showed 86.6% and 93.3% protection rates, respectively. Also, the protection rate against challenges with virulent Ornithobacterium rhinotracheale serotype A reached 96.6%.
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| first_indexed | 2024-03-11T17:38:51Z |
| format | Article |
| id | doaj.art-7b096e98b65142ca876bd53af7a7187d |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:38:51Z |
| publishDate | 2023-06-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-7b096e98b65142ca876bd53af7a7187d2023-10-18T14:13:50ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-06-01135Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in ChickensSally Roshdy0Rafik Soliman1Manal Aly2Lamiaa Omar3Ahmed Sameer4Heidy Abo-Elyazeed5Hassan Aboul-Ella6Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Ministry of Agriculture, Egypt. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.Department of Poultry Disease, Faculty of Veterinary Medicine, Cairo University, Egypt.Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Ministry of Agriculture, Egypt. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt. Poultry, mainly chickens, and their white meat represent one of the main, nutritionally valuable, and affordable red meat replacer sources of protein throughout the whole world with special reference to developing countries. A long list of microbial agents especially bacterial pathogens threatens chickens’ production cycles. They constitute one of the major problems facing the rapidly expanding poultry industry and are responsible for considerable economic losses. Fowl cholera, infectious coryza, and ornithobacteriosis (ORT) were among the serious bacterial infections that affect the respiratory tract of chickens with a global adverse effect on poultry production. A formalinized whole culture vaccine composed of Pasteurella multocida serotypes A5, A8, A9, and D2, Avibacterium paragallinarum serotypes A and C, and Ornithobacterium rhinotracheale serotype A was prepared. This polyvalent vaccine proved to be safe producing no adverse side effects when injected in chickens. The immunizing efficacy of this vaccine was evaluated in specific pathogen-free (SPF) chickens, which were immunized at 6 weeks of age. The protective efficacy of the vaccine was determined using a challenge test. The developed vaccine was effective in protecting chickens against fowl cholera, infectious coryza, and ornithobacteriosis in chickens against challenge with these pathogens. Vaccinated chickens challenged with virulent Pasteurella multocida serotypes A5, A8, A9, and D2 showed protection rates of 86.6%, 93.3%, 93.3%, and 93.3%, respectively, as compared with 100% mortality in the non-vaccinated control. Vaccinated chickens challenged with Avibacterium paragallinarum serotypes A and C showed 86.6% and 93.3% protection rates, respectively. Also, the protection rate against challenges with virulent Ornithobacterium rhinotracheale serotype A reached 96.6%. https://advetresearch.com/index.php/AVR/article/view/1320Trivalent vaccinePoultry infectious diseasesBacterial respiratory pathogenseconomic white meat sourceimmunizing efficacy |
| spellingShingle | Sally Roshdy Rafik Soliman Manal Aly Lamiaa Omar Ahmed Sameer Heidy Abo-Elyazeed Hassan Aboul-Ella Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens Journal of Advanced Veterinary Research Trivalent vaccine Poultry infectious diseases Bacterial respiratory pathogens economic white meat source immunizing efficacy |
| title | Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens |
| title_full | Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens |
| title_fullStr | Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens |
| title_full_unstemmed | Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens |
| title_short | Preparation of a Newly Developed Trivalent Pasteurella multocida, Avibacterium paragallinarum, and Ornithobacterium rhinotracheale Vaccine with an Evaluation of its Protective Efficacy in Chickens |
| title_sort | preparation of a newly developed trivalent pasteurella multocida avibacterium paragallinarum and ornithobacterium rhinotracheale vaccine with an evaluation of its protective efficacy in chickens |
| topic | Trivalent vaccine Poultry infectious diseases Bacterial respiratory pathogens economic white meat source immunizing efficacy |
| url | https://advetresearch.com/index.php/AVR/article/view/1320 |
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