Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers
The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the manageme...
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| Format: | Article |
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PeerJ Inc.
2016-05-01
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| Online Access: | https://peerj.com/articles/1894.pdf |
| _version_ | 1797418600726790144 |
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| author | Nikolett Sziszkosz Sándor Mihók András Jávor Szilvia Kusza |
| author_facet | Nikolett Sziszkosz Sándor Mihók András Jávor Szilvia Kusza |
| author_sort | Nikolett Sziszkosz |
| collection | DOAJ |
| description | The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp of CYTB and 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novel CYTB and four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 ± 0.011; D-loop: 0.9136 ± 0.008) and moderate nucleotide (CYTB: 0.00472 ± 0.00017; D-loop: 0.02091 ± 0.00068) diversity. Of the 31 Gidran mare families, only 15 CYTB (48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularly when the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran. |
| first_indexed | 2024-03-09T06:36:14Z |
| format | Article |
| id | doaj.art-7b0dcb581bd64015b74f1c7c1057cfcb |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:36:14Z |
| publishDate | 2016-05-01 |
| publisher | PeerJ Inc. |
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| series | PeerJ |
| spelling | doaj.art-7b0dcb581bd64015b74f1c7c1057cfcb2023-12-03T10:58:37ZengPeerJ Inc.PeerJ2167-83592016-05-014e189410.7717/peerj.1894Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markersNikolett Sziszkosz0Sándor Mihók1András Jávor2Szilvia Kusza3Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, Debrecen, HungaryInstitute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, Debrecen, HungaryInstitute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, Debrecen, HungaryInstitute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, Debrecen, HungaryThe Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp of CYTB and 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novel CYTB and four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 ± 0.011; D-loop: 0.9136 ± 0.008) and moderate nucleotide (CYTB: 0.00472 ± 0.00017; D-loop: 0.02091 ± 0.00068) diversity. Of the 31 Gidran mare families, only 15 CYTB (48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularly when the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran.https://peerj.com/articles/1894.pdfCYTBDiversityD-loopGidranStud book error |
| spellingShingle | Nikolett Sziszkosz Sándor Mihók András Jávor Szilvia Kusza Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers PeerJ CYTB Diversity D-loop Gidran Stud book error |
| title | Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers |
| title_full | Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers |
| title_fullStr | Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers |
| title_full_unstemmed | Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers |
| title_short | Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers |
| title_sort | genetic diversity of the hungarian gidran horse in two mitochondrial dna markers |
| topic | CYTB Diversity D-loop Gidran Stud book error |
| url | https://peerj.com/articles/1894.pdf |
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