Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids
Background: Semen identification is assumed a crucial proof of sexual assault. Moreover, body fluids at the crime scene of a human being, such as blood, semen, and saliva, are often mixed. Methods: Hence, in our study, we aimed to use methylation analysis targeting DNA epigenetic markers Spectrin be...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shaheed Beheshti University of Medical Sciences
2022-11-01
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| Series: | International Journal of Medical Toxicology and Forensic Medicine |
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| Online Access: | https://journals.sbmu.ac.ir/ijmtfm/article/view/38615 |
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| author | Abrar Roshdy Abouelkeir Abla Abdel Alrahman Ali Mokhtar Fathi Abdelsatar Laila Ahmed Rashed Shimaa Ahmed Alsaeed |
| author_facet | Abrar Roshdy Abouelkeir Abla Abdel Alrahman Ali Mokhtar Fathi Abdelsatar Laila Ahmed Rashed Shimaa Ahmed Alsaeed |
| author_sort | Abrar Roshdy Abouelkeir |
| collection | DOAJ |
| description | Background: Semen identification is assumed a crucial proof of sexual assault. Moreover, body fluids at the crime scene of a human being, such as blood, semen, and saliva, are often mixed.
Methods: Hence, in our study, we aimed to use methylation analysis targeting DNA epigenetic markers Spectrin beta chain (B_SPTB_03) and DEAD-box protein (DDX4) to differentiate between fresh semen (less than 4 hours) and aged semen (after 24 hours) as well as to differentiate between semen alone and semen mixed with other body fluids (blood and saliva) in the fresh and dried state.
Results: Our findings showed statistically significant differences in the methylation patterns of the SPTB and DDX4 loci to distinguish semen from mixed body fluids in fresh and old samples. We were able to obtain two novel cutoff values to differentiate between fresh and aged semen, which are (52.25) with the SPTB marker and (70.75) with the DDX4 marker.
Conclusion: It is concluded that the methylation approach based on the epigenetic markers of Spectrin beta chain and DEAD-box protein (B_SPTB_03 and DDX4) successfully identified fresh from aged semen and semen-derived alleles from mixed stains, hence it is recommended to be employed in forensic practice. |
| first_indexed | 2024-04-13T11:19:57Z |
| format | Article |
| id | doaj.art-7b0e0e02fe944910a0a719b5a406b27d |
| institution | Directory Open Access Journal |
| issn | 2251-8762 2251-8770 |
| language | English |
| last_indexed | 2024-04-13T11:19:57Z |
| publishDate | 2022-11-01 |
| publisher | Shaheed Beheshti University of Medical Sciences |
| record_format | Article |
| series | International Journal of Medical Toxicology and Forensic Medicine |
| spelling | doaj.art-7b0e0e02fe944910a0a719b5a406b27d2022-12-22T02:48:51ZengShaheed Beheshti University of Medical SciencesInternational Journal of Medical Toxicology and Forensic Medicine2251-87622251-87702022-11-01124386153861510.32598/ijmtfm.v12i4.3861538615Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body FluidsAbrar Roshdy AbouelkeirAbla Abdel Alrahman Alihttps://orcid.org/0000-0002-0870-1245Mokhtar Fathi AbdelsatarLaila Ahmed RashedShimaa Ahmed Alsaeedhttps://orcid.org/0000-0002-8479-5108Background: Semen identification is assumed a crucial proof of sexual assault. Moreover, body fluids at the crime scene of a human being, such as blood, semen, and saliva, are often mixed. Methods: Hence, in our study, we aimed to use methylation analysis targeting DNA epigenetic markers Spectrin beta chain (B_SPTB_03) and DEAD-box protein (DDX4) to differentiate between fresh semen (less than 4 hours) and aged semen (after 24 hours) as well as to differentiate between semen alone and semen mixed with other body fluids (blood and saliva) in the fresh and dried state. Results: Our findings showed statistically significant differences in the methylation patterns of the SPTB and DDX4 loci to distinguish semen from mixed body fluids in fresh and old samples. We were able to obtain two novel cutoff values to differentiate between fresh and aged semen, which are (52.25) with the SPTB marker and (70.75) with the DDX4 marker. Conclusion: It is concluded that the methylation approach based on the epigenetic markers of Spectrin beta chain and DEAD-box protein (B_SPTB_03 and DDX4) successfully identified fresh from aged semen and semen-derived alleles from mixed stains, hence it is recommended to be employed in forensic practice.https://journals.sbmu.ac.ir/ijmtfm/article/view/38615body fluid identificationdna methylationforensic applicationspectrin beta (sptb)dead-box protein (ddx4) |
| spellingShingle | Abrar Roshdy Abouelkeir Abla Abdel Alrahman Ali Mokhtar Fathi Abdelsatar Laila Ahmed Rashed Shimaa Ahmed Alsaeed Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids International Journal of Medical Toxicology and Forensic Medicine body fluid identification dna methylation forensic application spectrin beta (sptb) dead-box protein (ddx4) |
| title | Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids |
| title_full | Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids |
| title_fullStr | Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids |
| title_full_unstemmed | Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids |
| title_short | Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids |
| title_sort | application of the methylated markers spectrin beta and dead box protein for definitive differentiation between fresh and aged semen by evaluating their role in identifying semen from mixed body fluids |
| topic | body fluid identification dna methylation forensic application spectrin beta (sptb) dead-box protein (ddx4) |
| url | https://journals.sbmu.ac.ir/ijmtfm/article/view/38615 |
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