Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels
Maternal smoking during pregnancy may affect newborn DNA methylation (DNAm). However, little is known about how these associations vary by a newborn’s sex and/or maternal nutrition. To fill in this research gap, we investigated epigenome-wide DNAm associations with maternal smoking during pregnancy...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-05-01
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| Series: | Epigenetics |
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| Online Access: | http://dx.doi.org/10.1080/15592294.2018.1475978 |
| _version_ | 1797678962561777664 |
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| author | Boyang Zhang Xiumei Hong Hongkai Ji Wan-yee Tang Mary Kimmel Yuelong Ji Colleen Pearson Barry Zuckerman Pamela J. Surkan Xiaobin Wang |
| author_facet | Boyang Zhang Xiumei Hong Hongkai Ji Wan-yee Tang Mary Kimmel Yuelong Ji Colleen Pearson Barry Zuckerman Pamela J. Surkan Xiaobin Wang |
| author_sort | Boyang Zhang |
| collection | DOAJ |
| description | Maternal smoking during pregnancy may affect newborn DNA methylation (DNAm). However, little is known about how these associations vary by a newborn’s sex and/or maternal nutrition. To fill in this research gap, we investigated epigenome-wide DNAm associations with maternal smoking during pregnancy in African American mother-newborn pairs. DNAm profiling in cord (n = 379) and maternal blood (n = 300) were performed using the Illumina HumanMethylation450 BeadChip array. We identified 12 CpG sites whose DNAm levels in cord blood were associated with maternal smoking, at a false discovery rate <5%. The identified associations in the GFI1 gene were more pronounced in male newborns than in females (P = 0.002 for maternal smoking × sex interaction at cg18146737). We further observed that maternal smoking and folate level may interactively affect cord blood DNAm level at cg05575921 in the AHRR gene (P = 5.0 × 10−4 for interaction): compared to newborns unexposed to maternal smoking and with a high maternal folate level (>19.2 nmol/L), the DNAm level was about 0.03 lower (P = 3.6 × 10−4) in exposed newborns with a high maternal folate level, but was 0.08 lower (P = 1.2 × 10−8) in exposed newborns with a low maternal folate level. Our data suggest that adequate maternal folate levels may partly counteract the impact of maternal smoking on DNAm. These findings may open new avenues of inquiry regarding sex differences in response to environmental insults and novel strategies to mitigate their intergenerational health effects through optimization of maternal nutrition. |
| first_indexed | 2024-03-11T23:06:34Z |
| format | Article |
| id | doaj.art-7b0e75acf63d40bca0bdfd9428e82831 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:06:34Z |
| publishDate | 2018-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-7b0e75acf63d40bca0bdfd9428e828312023-09-21T13:09:20ZengTaylor & Francis GroupEpigenetics1559-22941559-23082018-05-0113550551810.1080/15592294.2018.14759781475978Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levelsBoyang Zhang0Xiumei Hong1Hongkai Ji2Wan-yee Tang3Mary Kimmel4Yuelong Ji5Colleen Pearson6Barry Zuckerman7Pamela J. Surkan8Xiaobin Wang9Johns Hopkins University Bloomberg School of Public HealthJohns Hopkins University Bloomberg School of Public HealthJohns Hopkins University Bloomberg School of Public HealthJohns Hopkins University Bloomberg School of Public HealthUniversity of North Carolina at Chapel Hill’s School of MedicineJohns Hopkins University Bloomberg School of Public HealthBoston University School of Medicine and Boston Medical CenterBoston University School of Medicine and Boston Medical CenterJohns Hopkins Bloomberg School of Public HealthJohns Hopkins University Bloomberg School of Public HealthMaternal smoking during pregnancy may affect newborn DNA methylation (DNAm). However, little is known about how these associations vary by a newborn’s sex and/or maternal nutrition. To fill in this research gap, we investigated epigenome-wide DNAm associations with maternal smoking during pregnancy in African American mother-newborn pairs. DNAm profiling in cord (n = 379) and maternal blood (n = 300) were performed using the Illumina HumanMethylation450 BeadChip array. We identified 12 CpG sites whose DNAm levels in cord blood were associated with maternal smoking, at a false discovery rate <5%. The identified associations in the GFI1 gene were more pronounced in male newborns than in females (P = 0.002 for maternal smoking × sex interaction at cg18146737). We further observed that maternal smoking and folate level may interactively affect cord blood DNAm level at cg05575921 in the AHRR gene (P = 5.0 × 10−4 for interaction): compared to newborns unexposed to maternal smoking and with a high maternal folate level (>19.2 nmol/L), the DNAm level was about 0.03 lower (P = 3.6 × 10−4) in exposed newborns with a high maternal folate level, but was 0.08 lower (P = 1.2 × 10−8) in exposed newborns with a low maternal folate level. Our data suggest that adequate maternal folate levels may partly counteract the impact of maternal smoking on DNAm. These findings may open new avenues of inquiry regarding sex differences in response to environmental insults and novel strategies to mitigate their intergenerational health effects through optimization of maternal nutrition.http://dx.doi.org/10.1080/15592294.2018.1475978maternal smoking during pregnancydna methylationafrican americansex differencematernal folateeffect modification |
| spellingShingle | Boyang Zhang Xiumei Hong Hongkai Ji Wan-yee Tang Mary Kimmel Yuelong Ji Colleen Pearson Barry Zuckerman Pamela J. Surkan Xiaobin Wang Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels Epigenetics maternal smoking during pregnancy dna methylation african american sex difference maternal folate effect modification |
| title | Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels |
| title_full | Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels |
| title_fullStr | Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels |
| title_full_unstemmed | Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels |
| title_short | Maternal smoking during pregnancy and cord blood DNA methylation: new insight on sex differences and effect modification by maternal folate levels |
| title_sort | maternal smoking during pregnancy and cord blood dna methylation new insight on sex differences and effect modification by maternal folate levels |
| topic | maternal smoking during pregnancy dna methylation african american sex difference maternal folate effect modification |
| url | http://dx.doi.org/10.1080/15592294.2018.1475978 |
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