Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance
Colon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, specifically bacterial cyclomodulin positivity (CM<sup>+</sup>). Unfortunately, there are no...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2076-0817/11/1/14 |
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| author | Adrian Canizalez-Roman Juan E. Reina-Reyes Uriel A. Angulo-Zamudio Eloy E. Geminiano-Martínez Antonio F. Flores-Carrillo Rolando R. García-Matus Norma M. Valencia-Mijares Nidia Leon-Sicairos Jorge Velazquez-Roman Francisco A. Martínez-Villa Gabriela Tapia-Pastrana |
| author_facet | Adrian Canizalez-Roman Juan E. Reina-Reyes Uriel A. Angulo-Zamudio Eloy E. Geminiano-Martínez Antonio F. Flores-Carrillo Rolando R. García-Matus Norma M. Valencia-Mijares Nidia Leon-Sicairos Jorge Velazquez-Roman Francisco A. Martínez-Villa Gabriela Tapia-Pastrana |
| author_sort | Adrian Canizalez-Roman |
| collection | DOAJ |
| description | Colon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, specifically bacterial cyclomodulin positivity (CM<sup>+</sup>). Unfortunately, there are no studies from Mexico that detail the presence of bacterial CM<sup>+</sup> in patients with colon diseases. We therefore performed a comprehensive study to investigate the associations and prevalence of cyclomodulin-positive Diarrheagenic <i>E. coli</i> (DEC)<i>,</i> non-DEC, and <i>Klebsiella</i> spp. strains isolated from Mexican subjects with colon diseases. In this work, we analyzed 43 biopsies, 87 different bacteria were isolated, and <i>E. coli</i> was the most frequently noted, followed by <i>Klebsiella</i> spp., and <i>Enterococcus</i> spp. <i>E. coli</i>, non-DEC, and EPEC belonging to phylogroup B2 were the most prevalent. More than 80% of <i>E. coli</i> and <i>Klebsiella</i> were CM<sup>+</sup>. <i>pks</i>, <i>cdt</i>, <i>cnf</i>, and <i>cif</i> were identified. <i>cdt</i> was associated with non-DEC, <i>cif</i> and its combinations with EPEC, as well as <i>cdt</i> and <i>psk</i> with <i>Klebsiella</i>. Lastly, all the CM<sup>+</sup> bacteria were resistant to at least one antibiotic (34% were MDR, and 48% XDR). In conclusion, the high prevalence of bacterial CM<sup>+</sup> in colon disease patients suggests that these bacteria play an important role in the genesis of these diseases. |
| first_indexed | 2024-03-10T00:46:12Z |
| format | Article |
| id | doaj.art-7b10d4fb1cd4409eb86bf53080ed2ea6 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T00:46:12Z |
| publishDate | 2021-12-01 |
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| series | Pathogens |
| spelling | doaj.art-7b10d4fb1cd4409eb86bf53080ed2ea62023-11-23T14:59:04ZengMDPI AGPathogens2076-08172021-12-011111410.3390/pathogens11010014Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial ResistanceAdrian Canizalez-Roman0Juan E. Reina-Reyes1Uriel A. Angulo-Zamudio2Eloy E. Geminiano-Martínez3Antonio F. Flores-Carrillo4Rolando R. García-Matus5Norma M. Valencia-Mijares6Nidia Leon-Sicairos7Jorge Velazquez-Roman8Francisco A. Martínez-Villa9Gabriela Tapia-Pastrana10Centro de Investigación Aplicada a la Salud Pública (CIASaP), School of Medicine, Autonomous University of Sinaloa, Culiacan Sinaloa 80246, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca City 71256, MexicoCentro de Investigación Aplicada a la Salud Pública (CIASaP), School of Medicine, Autonomous University of Sinaloa, Culiacan Sinaloa 80246, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca City 71256, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca City 71256, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca City 71256, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca City 71256, MexicoCentro de Investigación Aplicada a la Salud Pública (CIASaP), School of Medicine, Autonomous University of Sinaloa, Culiacan Sinaloa 80246, MexicoCentro de Investigación Aplicada a la Salud Pública (CIASaP), School of Medicine, Autonomous University of Sinaloa, Culiacan Sinaloa 80246, MexicoPrograma de Maestría en Ciencias en Biomedicina Molecular, Facultad de Medicina, UAS, Culiacan Sinaloa 80246, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca City 71256, MexicoColon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, specifically bacterial cyclomodulin positivity (CM<sup>+</sup>). Unfortunately, there are no studies from Mexico that detail the presence of bacterial CM<sup>+</sup> in patients with colon diseases. We therefore performed a comprehensive study to investigate the associations and prevalence of cyclomodulin-positive Diarrheagenic <i>E. coli</i> (DEC)<i>,</i> non-DEC, and <i>Klebsiella</i> spp. strains isolated from Mexican subjects with colon diseases. In this work, we analyzed 43 biopsies, 87 different bacteria were isolated, and <i>E. coli</i> was the most frequently noted, followed by <i>Klebsiella</i> spp., and <i>Enterococcus</i> spp. <i>E. coli</i>, non-DEC, and EPEC belonging to phylogroup B2 were the most prevalent. More than 80% of <i>E. coli</i> and <i>Klebsiella</i> were CM<sup>+</sup>. <i>pks</i>, <i>cdt</i>, <i>cnf</i>, and <i>cif</i> were identified. <i>cdt</i> was associated with non-DEC, <i>cif</i> and its combinations with EPEC, as well as <i>cdt</i> and <i>psk</i> with <i>Klebsiella</i>. Lastly, all the CM<sup>+</sup> bacteria were resistant to at least one antibiotic (34% were MDR, and 48% XDR). In conclusion, the high prevalence of bacterial CM<sup>+</sup> in colon disease patients suggests that these bacteria play an important role in the genesis of these diseases.https://www.mdpi.com/2076-0817/11/1/14cyclomodulinscolon diseases<i>E. coli</i>DEC<i>Klebsiella</i> spp. |
| spellingShingle | Adrian Canizalez-Roman Juan E. Reina-Reyes Uriel A. Angulo-Zamudio Eloy E. Geminiano-Martínez Antonio F. Flores-Carrillo Rolando R. García-Matus Norma M. Valencia-Mijares Nidia Leon-Sicairos Jorge Velazquez-Roman Francisco A. Martínez-Villa Gabriela Tapia-Pastrana Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance Pathogens cyclomodulins colon diseases <i>E. coli</i> DEC <i>Klebsiella</i> spp. |
| title | Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance |
| title_full | Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance |
| title_fullStr | Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance |
| title_full_unstemmed | Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance |
| title_short | Prevalence of Cyclomodulin-Positive <i>E. coli</i> and <i>Klebsiella</i> spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance |
| title_sort | prevalence of cyclomodulin positive i e coli i and i klebsiella i spp strains in mexican patients with colon diseases and antimicrobial resistance |
| topic | cyclomodulins colon diseases <i>E. coli</i> DEC <i>Klebsiella</i> spp. |
| url | https://www.mdpi.com/2076-0817/11/1/14 |
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