Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection
<p>Abstract</p> <p>Background</p> <p>Adhesion of <it>Plasmodium</it>-infected red blood cells (iRBC) to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. <it>In vitro </it>studies have shown th...
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| Format: | Article |
| Language: | English |
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BMC
2007-03-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/6/1/32 |
| _version_ | 1818753267298992128 |
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| author | Febbraio Maria Portugal Sílvia Cunha-Rodrigues Margarida Mota Maria M |
| author_facet | Febbraio Maria Portugal Sílvia Cunha-Rodrigues Margarida Mota Maria M |
| author_sort | Febbraio Maria |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Adhesion of <it>Plasmodium</it>-infected red blood cells (iRBC) to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. <it>In vitro </it>studies have shown the relevance of CD36 for adhesion phenotypes of <it>Plasmodium falciparum </it>iRBC such as sequestration, platelet mediated clumping and non-opsonic uptake of iRBC. Different adhesion phenotypes involve different host cells and are associated with different pathological outcomes of disease. Studies with different human populations with CD36 polymorphisms failed to attribute a clear role to CD36 expression in human malaria. Up to the present, no <it>in vivo </it>model has been available to study the relevance of different CD36 adhesion phenotypes to the pathological course of <it>Plasmodium </it>infection.</p> <p>Methods</p> <p>Using CD36-deficient mice and their control littermates, CD36 bone marrow chimeric mice, expressing CD36 exclusively in haematopoietic cells or in non-haematopoietic cells, were generated. Irradiated CD36<sup>-/- </sup>and wild type mice were also reconstituted with syngeneic cells to control for the effects of irradiation. The reconstituted mice were infected with <it>Plasmodium berghei </it>ANKA and analysed for the development of blood parasitaemia and neurological symptoms.</p> <p>Results</p> <p>All mice reconstituted with syngeneic bone marrow cells as well as chimeric mice expressing CD36 exclusively in non-haematopoietic cells died from experimental cerebral malaria between day 6 and 12 after infection. A significant proportion of chimeric mice expressing CD36 only in haematopoietic cells did not die from cerebral malaria.</p> <p>Conclusion</p> <p>The analysis of bone marrow chimeric mice reveals a dual role of CD36 in <it>P. berghei ANKA </it>infection. Expression of CD36 in haematopoietic cells, most likely macrophages and dendritic cells, has a beneficial effect that is masked in normal mice by adverse effects of CD36 expression in non-haematopoietic cells, most likely endothelial cells.</p> |
| first_indexed | 2024-12-18T05:04:38Z |
| format | Article |
| id | doaj.art-7b12c921cc8d47c2b1b480c9b60b3f01 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-18T05:04:38Z |
| publishDate | 2007-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-7b12c921cc8d47c2b1b480c9b60b3f012022-12-21T21:20:04ZengBMCMalaria Journal1475-28752007-03-01613210.1186/1475-2875-6-32Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infectionFebbraio MariaPortugal SílviaCunha-Rodrigues MargaridaMota Maria M<p>Abstract</p> <p>Background</p> <p>Adhesion of <it>Plasmodium</it>-infected red blood cells (iRBC) to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. <it>In vitro </it>studies have shown the relevance of CD36 for adhesion phenotypes of <it>Plasmodium falciparum </it>iRBC such as sequestration, platelet mediated clumping and non-opsonic uptake of iRBC. Different adhesion phenotypes involve different host cells and are associated with different pathological outcomes of disease. Studies with different human populations with CD36 polymorphisms failed to attribute a clear role to CD36 expression in human malaria. Up to the present, no <it>in vivo </it>model has been available to study the relevance of different CD36 adhesion phenotypes to the pathological course of <it>Plasmodium </it>infection.</p> <p>Methods</p> <p>Using CD36-deficient mice and their control littermates, CD36 bone marrow chimeric mice, expressing CD36 exclusively in haematopoietic cells or in non-haematopoietic cells, were generated. Irradiated CD36<sup>-/- </sup>and wild type mice were also reconstituted with syngeneic cells to control for the effects of irradiation. The reconstituted mice were infected with <it>Plasmodium berghei </it>ANKA and analysed for the development of blood parasitaemia and neurological symptoms.</p> <p>Results</p> <p>All mice reconstituted with syngeneic bone marrow cells as well as chimeric mice expressing CD36 exclusively in non-haematopoietic cells died from experimental cerebral malaria between day 6 and 12 after infection. A significant proportion of chimeric mice expressing CD36 only in haematopoietic cells did not die from cerebral malaria.</p> <p>Conclusion</p> <p>The analysis of bone marrow chimeric mice reveals a dual role of CD36 in <it>P. berghei ANKA </it>infection. Expression of CD36 in haematopoietic cells, most likely macrophages and dendritic cells, has a beneficial effect that is masked in normal mice by adverse effects of CD36 expression in non-haematopoietic cells, most likely endothelial cells.</p>http://www.malariajournal.com/content/6/1/32 |
| spellingShingle | Febbraio Maria Portugal Sílvia Cunha-Rodrigues Margarida Mota Maria M Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection Malaria Journal |
| title | Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection |
| title_full | Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection |
| title_fullStr | Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection |
| title_full_unstemmed | Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection |
| title_short | Bone marrow chimeric mice reveal a dual role for CD36 in <it>Plasmodium berghei </it>ANKA infection |
| title_sort | bone marrow chimeric mice reveal a dual role for cd36 in it plasmodium berghei it anka infection |
| url | http://www.malariajournal.com/content/6/1/32 |
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