BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape
Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antivir...
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MDPI AG
2015-07-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/8/2815 |
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| author | Quan Chen Jie Wang Mingsheng Hou Shengyi Liu Junyan Huang Li Cai |
| author_facet | Quan Chen Jie Wang Mingsheng Hou Shengyi Liu Junyan Huang Li Cai |
| author_sort | Quan Chen |
| collection | DOAJ |
| description | Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties. |
| first_indexed | 2024-12-23T13:40:53Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-23T13:40:53Z |
| publishDate | 2015-07-01 |
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| series | Viruses |
| spelling | doaj.art-7b14951f1ef7421189fc6db1a32c1b882022-12-21T17:44:52ZengMDPI AGViruses1999-49152015-07-01784169418510.3390/v7082815v7082815BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed RapeQuan Chen0Jie Wang1Mingsheng Hou2Shengyi Liu3Junyan Huang4Li Cai5College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biologyand Genetic Breeding of Oil Crops, the Ministry of Agriculture, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biologyand Genetic Breeding of Oil Crops, the Ministry of Agriculture, Wuhan 430062, ChinaCollege of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, ChinaVirus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties.http://www.mdpi.com/1999-4915/7/8/2815oilseed rapevirusBnSGS3virus accumulationbreeding strategy |
| spellingShingle | Quan Chen Jie Wang Mingsheng Hou Shengyi Liu Junyan Huang Li Cai BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape Viruses oilseed rape virus BnSGS3 virus accumulation breeding strategy |
| title | BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape |
| title_full | BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape |
| title_fullStr | BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape |
| title_full_unstemmed | BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape |
| title_short | BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape |
| title_sort | bnsgs3 has differential effects on the accumulation of cmv ormv and tumv in oilseed rape |
| topic | oilseed rape virus BnSGS3 virus accumulation breeding strategy |
| url | http://www.mdpi.com/1999-4915/7/8/2815 |
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