Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani
Sheath blight is a serious rice disease worldwide, and genes involved in resistance remain unclear. In the present study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of a sheath blight-resistant rice cultivar, Jasmine 85; a suppression subtractive cDNA librar...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2022-06-01
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| Series: | PhytoFrontiers |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-21-0035-R |
| _version_ | 1797326729116647424 |
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| author | Youngjae Oh Seonghee Lee Renee Rioux Pratibha Singh Melissa H. Jia Yulin Jia Kirankumar S. Mysore |
| author_facet | Youngjae Oh Seonghee Lee Renee Rioux Pratibha Singh Melissa H. Jia Yulin Jia Kirankumar S. Mysore |
| author_sort | Youngjae Oh |
| collection | DOAJ |
| description | Sheath blight is a serious rice disease worldwide, and genes involved in resistance remain unclear. In the present study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of a sheath blight-resistant rice cultivar, Jasmine 85; a suppression subtractive cDNA library was constructed using RNA isolated 16 h postinoculation (hpi); and differentially expressed genes were identified from the cDNA library. A total of 159 uniquely expressed sequence tags were identified, including 105 from rice with enrichment in categories related to cellular response, molecular signaling, and host defense. Coupled with gene expression studies by DNA microarray, 27 highly induced genes involved in signal transduction and defense responses were identified within 16 hpi. Three members of the ATP-binding cassette (ABC) transporter gene family (OsABC1, OsABC9, and OsABC12) encoding pleiotropic drug resistance-like ABC transporters were mapped to different sheath blight resistance quantitative trait loci (QTLs), and their differential expressions were validated. Three high-resolution melting (HRM) markers were developed from these ABC gene family members to distinguish alleles between sheath blight-susceptible cultivar Lemont and resistant cultivar Jasmine 85. Association of sheath blight resistance to these HRM markers was examined in 77 recombinant inbred lines derived from the cross between Jasmine 85 and Lemont. The OsABC9 gene located in a major sheath blight resistance QTL qShB9-2 showed a major contribution to sheath blight resistance. These results are useful for marker-assisted selection and functional validation of the ABC genes in sheath blight disease resistance. [Graphic: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2022. |
| first_indexed | 2024-03-08T06:28:21Z |
| format | Article |
| id | doaj.art-7b172ae9b757471e9dd70727c6a380d2 |
| institution | Directory Open Access Journal |
| issn | 2690-5442 |
| language | English |
| last_indexed | 2024-03-08T06:28:21Z |
| publishDate | 2022-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | PhytoFrontiers |
| spelling | doaj.art-7b172ae9b757471e9dd70727c6a380d22024-02-03T13:28:30ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422022-06-012210511510.1094/PHYTOFR-05-21-0035-RAnalysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solaniYoungjae Oh0Seonghee Lee1Renee Rioux2Pratibha Singh3Melissa H. Jia4Yulin Jia5Kirankumar S. Mysore6Department of Horticultural Science, University of Florida, IFAS Gulf Coast Research and Education Center, Wimauma, FL 33598, U.S.A.Department of Horticultural Science, University of Florida, IFAS Gulf Coast Research and Education Center, Wimauma, FL 33598, U.S.A.Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, U.S.A.University of Arkansas Rice Research and Extension Center, Stuttgart, AR 72160, U.S.A.USDA-ARS Dale Bumpers National Rice Research Center (DB NRRC), Stuttgart, AR 72160, U.S.A.USDA-ARS Dale Bumpers National Rice Research Center (DB NRRC), Stuttgart, AR 72160, U.S.A.Noble Research Institute, LLC, Ardmore, OK 73401, U.S.A.Sheath blight is a serious rice disease worldwide, and genes involved in resistance remain unclear. In the present study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of a sheath blight-resistant rice cultivar, Jasmine 85; a suppression subtractive cDNA library was constructed using RNA isolated 16 h postinoculation (hpi); and differentially expressed genes were identified from the cDNA library. A total of 159 uniquely expressed sequence tags were identified, including 105 from rice with enrichment in categories related to cellular response, molecular signaling, and host defense. Coupled with gene expression studies by DNA microarray, 27 highly induced genes involved in signal transduction and defense responses were identified within 16 hpi. Three members of the ATP-binding cassette (ABC) transporter gene family (OsABC1, OsABC9, and OsABC12) encoding pleiotropic drug resistance-like ABC transporters were mapped to different sheath blight resistance quantitative trait loci (QTLs), and their differential expressions were validated. Three high-resolution melting (HRM) markers were developed from these ABC gene family members to distinguish alleles between sheath blight-susceptible cultivar Lemont and resistant cultivar Jasmine 85. Association of sheath blight resistance to these HRM markers was examined in 77 recombinant inbred lines derived from the cross between Jasmine 85 and Lemont. The OsABC9 gene located in a major sheath blight resistance QTL qShB9-2 showed a major contribution to sheath blight resistance. These results are useful for marker-assisted selection and functional validation of the ABC genes in sheath blight disease resistance. [Graphic: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2022.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-21-0035-Rdisease resistancehigh-resolution meltingOryza sativaquantitative trait loci (QTLs)rice blastsheath blight |
| spellingShingle | Youngjae Oh Seonghee Lee Renee Rioux Pratibha Singh Melissa H. Jia Yulin Jia Kirankumar S. Mysore Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani PhytoFrontiers disease resistance high-resolution melting Oryza sativa quantitative trait loci (QTLs) rice blast sheath blight |
| title | Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani |
| title_full | Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani |
| title_fullStr | Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani |
| title_full_unstemmed | Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani |
| title_short | Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani |
| title_sort | analysis of differentially expressed rice genes reveals the atp binding cassette transporters as candidate genes against the sheath blight pathogen rhizoctonia solani |
| topic | disease resistance high-resolution melting Oryza sativa quantitative trait loci (QTLs) rice blast sheath blight |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-21-0035-R |
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