Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus
Background and Objective: Sea cucumber (Holothuria leucospilota) is used for food purposes and traditional medicine in the South East and East Asia. This study was done to determine the antiviral effect of methanolic extract, of Holothuria leucospilota species against HIV-1 virus. Methods: In this l...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2015-12-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-877&slc_lang=en&sid=1 |
| _version_ | 1811282943919783936 |
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| author | Bahroudi S Nematollahi MA Aghasadeghi MR Nazemi M Bahroudi M Behrouz B |
| author_facet | Bahroudi S Nematollahi MA Aghasadeghi MR Nazemi M Bahroudi M Behrouz B |
| author_sort | Bahroudi S |
| collection | DOAJ |
| description | Background and Objective: Sea cucumber (Holothuria leucospilota) is used for food purposes and traditional medicine in the South East and East Asia. This study was done to determine the antiviral effect of methanolic extract, of Holothuria leucospilota species against HIV-1 virus. Methods: In this laboratory study, sea cucumbers were collected from Larak Island, Persian Gulf, Iran at depths of 10-30 m. Methanol solvent was used for extraction process. Extract was concentrated by rotary evaporator at 40-45 degree C, and subsequently was prepared in the form of dry powder using vacuum freeze dryer lyophilization. Results: The extract in 100 and 1000 µg/ml of concentrations inhibited by 94% and 92.5% the replication of HIV-1, respectively. 10 µg/ml of extract had not specific antiviral effect. Approximately the half of concentration of extract (35.89 µg/ml) prevents 50% of proliferation of HIV-1, which was 50% toxic of on host cells (P<0.05). Conclusion: Sea cucumber methanolic body wall extract of Holothuria leucospilota species had no antiviral effect against HIV-1 virus. It can be due to cytotoxic effect of extract on the host cells. |
| first_indexed | 2024-04-13T02:01:42Z |
| format | Article |
| id | doaj.art-7b1783cafeb1446ebaa93235ebd9391b |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-04-13T02:01:42Z |
| publishDate | 2015-12-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-7b1783cafeb1446ebaa93235ebd9391b2022-12-22T03:07:35ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802015-12-01174125131Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virusBahroudi S0Nematollahi MA1Aghasadeghi MR2Nazemi M3Bahroudi M4Behrouz B5 M.Sc in Processing Marine Products, Fisheries Department, Faculty of Natural Resources, University of Tehran, Karaj, Iran Associate Professor, Fisheries Department, Faculty of Natural Resources, University of Tehran, Karaj, Iran Associate Professor, Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, IR Iran Assistant Professor, Persian Gulf and Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran M.Sc in Microbiology, Microbiology Department, Tehran University of Medical Sciences, Tehran, Iran M.Sc in Microbiology, Microbiology Department, Tehran University of Medical Sciences, Tehran, Iran Background and Objective: Sea cucumber (Holothuria leucospilota) is used for food purposes and traditional medicine in the South East and East Asia. This study was done to determine the antiviral effect of methanolic extract, of Holothuria leucospilota species against HIV-1 virus. Methods: In this laboratory study, sea cucumbers were collected from Larak Island, Persian Gulf, Iran at depths of 10-30 m. Methanol solvent was used for extraction process. Extract was concentrated by rotary evaporator at 40-45 degree C, and subsequently was prepared in the form of dry powder using vacuum freeze dryer lyophilization. Results: The extract in 100 and 1000 µg/ml of concentrations inhibited by 94% and 92.5% the replication of HIV-1, respectively. 10 µg/ml of extract had not specific antiviral effect. Approximately the half of concentration of extract (35.89 µg/ml) prevents 50% of proliferation of HIV-1, which was 50% toxic of on host cells (P<0.05). Conclusion: Sea cucumber methanolic body wall extract of Holothuria leucospilota species had no antiviral effect against HIV-1 virus. It can be due to cytotoxic effect of extract on the host cells.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-877&slc_lang=en&sid=1Sea cucumberHIV-1 virus |
| spellingShingle | Bahroudi S Nematollahi MA Aghasadeghi MR Nazemi M Bahroudi M Behrouz B Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus مجله دانشگاه علوم پزشکی گرگان Sea cucumber HIV-1 virus |
| title | Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus |
| title_full | Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus |
| title_fullStr | Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus |
| title_full_unstemmed | Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus |
| title_short | Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus |
| title_sort | anti viral effect of methanolic extract of sea cucumber on hiv 1 virus |
| topic | Sea cucumber HIV-1 virus |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-877&slc_lang=en&sid=1 |
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