Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda
ABSTRACTArthropod-borne (arbo) viruses cause emerging diseases that affect the livelihoods of people around the world. They are linked to disease outbreaks resulting in high morbidity, mortality, and economic loss. In sub-Saharan Africa, numerous arbovirus outbreaks have been documented, but the cir...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Infection Ecology & Epidemiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20008686.2023.2289872 |
| _version_ | 1797400726702391296 |
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| author | Vincent Rusanganwa Olivia Wesula Lwande Brenda Bainda Patrick I. Chiyo Eric Seruyange Göran Bucht Magnus Evander |
| author_facet | Vincent Rusanganwa Olivia Wesula Lwande Brenda Bainda Patrick I. Chiyo Eric Seruyange Göran Bucht Magnus Evander |
| author_sort | Vincent Rusanganwa |
| collection | DOAJ |
| description | ABSTRACTArthropod-borne (arbo) viruses cause emerging diseases that affect the livelihoods of people around the world. They are linked to disease outbreaks resulting in high morbidity, mortality, and economic loss. In sub-Saharan Africa, numerous arbovirus outbreaks have been documented, but the circulation and magnitude of illness caused by these viruses during inter-epidemic periods remains unknown in many regions. In Rwanda, there is limited knowledge on the presence and distribution of arboviruses. This study aimed at determining the occurrence and distribution of selected arboviruses, i.e., chikungunya virus (CHIKV), o’nyong-nyong virus (ONNV), dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), Rift Valley fever virus (RVFV) and Crimean-Congo haemorrhagic fever virus (CCHFV), among febrile patients visiting health centres in Rwanda. A total of 2294 dry blood spots (DBS) were collected on filter papers during August 2019 – December 2020. Reverse-transcription polymerase chain reaction (RT-PCR) was performed on samples in pools of ten, using both quantitative (DENV, ZIKV, RVFV) and conventional PCR (CHIKV, ONNV, WNV, CCHFV) with virus specific primers, followed by sequencing. Demographic data and clinical manifestations of illness were analysed. ONNV infection was detected in 12 of 230 pools (5.2%) and ZIKV in three pools (1.3%). The other arboviruses were not detected. All ONNV cases were found in the Rwaniro health centre, while ZIKV infection was found among patients visiting the Kirinda and Zaza health centres. There was temporal variability in ONNV infections with most cases being recorded during the long dry season, while ZIKV infection occurred during both dry and wet seasons. Patients with ONNV were older and more were females. In conclusion, ONNV and ZIKV infection were detected in acute patients and can explain some of the feverish diseases in Rwanda. |
| first_indexed | 2024-03-09T01:59:42Z |
| format | Article |
| id | doaj.art-7b1c833bb31c4d3cacf09512e32311f8 |
| institution | Directory Open Access Journal |
| issn | 2000-8686 |
| language | English |
| last_indexed | 2024-03-09T01:59:42Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Infection Ecology & Epidemiology |
| spelling | doaj.art-7b1c833bb31c4d3cacf09512e32311f82023-12-08T07:26:18ZengTaylor & Francis GroupInfection Ecology & Epidemiology2000-86862024-12-0114110.1080/20008686.2023.2289872Arbovirus surveillance in febrile patients attending selected health facilities in RwandaVincent Rusanganwa0Olivia Wesula Lwande1Brenda Bainda2Patrick I. Chiyo3Eric Seruyange4Göran Bucht5Magnus Evander6College of Medicine and Health Sciences, University of Rwanda, Kigali, RwandaDepartment of Clinical Microbiology, Umeå University, Umeå, SwedenDepartment of Clinical Microbiology, Umeå University, Umeå, SwedenDepartment of Biology, Duke University, Durham, North Carolina, USACollege of Medicine and Health Sciences, University of Rwanda, Kigali, RwandaDepartment of Clinical Microbiology, Umeå University, Umeå, SwedenDepartment of Clinical Microbiology, Umeå University, Umeå, SwedenABSTRACTArthropod-borne (arbo) viruses cause emerging diseases that affect the livelihoods of people around the world. They are linked to disease outbreaks resulting in high morbidity, mortality, and economic loss. In sub-Saharan Africa, numerous arbovirus outbreaks have been documented, but the circulation and magnitude of illness caused by these viruses during inter-epidemic periods remains unknown in many regions. In Rwanda, there is limited knowledge on the presence and distribution of arboviruses. This study aimed at determining the occurrence and distribution of selected arboviruses, i.e., chikungunya virus (CHIKV), o’nyong-nyong virus (ONNV), dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), Rift Valley fever virus (RVFV) and Crimean-Congo haemorrhagic fever virus (CCHFV), among febrile patients visiting health centres in Rwanda. A total of 2294 dry blood spots (DBS) were collected on filter papers during August 2019 – December 2020. Reverse-transcription polymerase chain reaction (RT-PCR) was performed on samples in pools of ten, using both quantitative (DENV, ZIKV, RVFV) and conventional PCR (CHIKV, ONNV, WNV, CCHFV) with virus specific primers, followed by sequencing. Demographic data and clinical manifestations of illness were analysed. ONNV infection was detected in 12 of 230 pools (5.2%) and ZIKV in three pools (1.3%). The other arboviruses were not detected. All ONNV cases were found in the Rwaniro health centre, while ZIKV infection was found among patients visiting the Kirinda and Zaza health centres. There was temporal variability in ONNV infections with most cases being recorded during the long dry season, while ZIKV infection occurred during both dry and wet seasons. Patients with ONNV were older and more were females. In conclusion, ONNV and ZIKV infection were detected in acute patients and can explain some of the feverish diseases in Rwanda.https://www.tandfonline.com/doi/10.1080/20008686.2023.2289872Tick-borne virusesmosquito-borne virusesmolecular detectionarbovirusRwanda |
| spellingShingle | Vincent Rusanganwa Olivia Wesula Lwande Brenda Bainda Patrick I. Chiyo Eric Seruyange Göran Bucht Magnus Evander Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda Infection Ecology & Epidemiology Tick-borne viruses mosquito-borne viruses molecular detection arbovirus Rwanda |
| title | Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda |
| title_full | Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda |
| title_fullStr | Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda |
| title_full_unstemmed | Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda |
| title_short | Arbovirus surveillance in febrile patients attending selected health facilities in Rwanda |
| title_sort | arbovirus surveillance in febrile patients attending selected health facilities in rwanda |
| topic | Tick-borne viruses mosquito-borne viruses molecular detection arbovirus Rwanda |
| url | https://www.tandfonline.com/doi/10.1080/20008686.2023.2289872 |
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