Towards Point of Care CRISPR-Based Diagnostics: From Method to Device
Rapid, accurate, and portable on-site detection is critical in the face of public health emergencies. Infectious disease control and public health emergency policymaking can both be aided by effective and trustworthy point of care tests (POCT). A very promising POCT method appears to be the clustere...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/14/2/97 |
| _version_ | 1797620132938252288 |
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| author | Haoxiang Chen Xi Zhou Miao Wang Lei Ren |
| author_facet | Haoxiang Chen Xi Zhou Miao Wang Lei Ren |
| author_sort | Haoxiang Chen |
| collection | DOAJ |
| description | Rapid, accurate, and portable on-site detection is critical in the face of public health emergencies. Infectious disease control and public health emergency policymaking can both be aided by effective and trustworthy point of care tests (POCT). A very promising POCT method appears to be the clustered regularly interspaced short palindromic repeats and associated protein (CRISPR/Cas)-based molecular diagnosis. For on-site detection, CRISPR/Cas-based detection can be combined with multiple signal sensing methods and integrated into smart devices. In this review, sensing methods for CRISPR/Cas-based diagnostics are introduced and the advanced strategies and recent advances in CRISPR/Cas-based POCT are reviewed. Finally, the future perspectives of CRISPR and POCT are summarized and prospected. |
| first_indexed | 2024-03-11T08:37:07Z |
| format | Article |
| id | doaj.art-7b1e10e44ddf4e3f8bb9da5b29e1ab27 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T08:37:07Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-7b1e10e44ddf4e3f8bb9da5b29e1ab272023-11-16T21:24:37ZengMDPI AGJournal of Functional Biomaterials2079-49832023-02-011429710.3390/jfb14020097Towards Point of Care CRISPR-Based Diagnostics: From Method to DeviceHaoxiang Chen0Xi Zhou1Miao Wang2Lei Ren3The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, ChinaThe Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, ChinaThe Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, ChinaThe Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, ChinaRapid, accurate, and portable on-site detection is critical in the face of public health emergencies. Infectious disease control and public health emergency policymaking can both be aided by effective and trustworthy point of care tests (POCT). A very promising POCT method appears to be the clustered regularly interspaced short palindromic repeats and associated protein (CRISPR/Cas)-based molecular diagnosis. For on-site detection, CRISPR/Cas-based detection can be combined with multiple signal sensing methods and integrated into smart devices. In this review, sensing methods for CRISPR/Cas-based diagnostics are introduced and the advanced strategies and recent advances in CRISPR/Cas-based POCT are reviewed. Finally, the future perspectives of CRISPR and POCT are summarized and prospected.https://www.mdpi.com/2079-4983/14/2/97CRISPR/Casmolecular diagnosticpoint of careDNA detection |
| spellingShingle | Haoxiang Chen Xi Zhou Miao Wang Lei Ren Towards Point of Care CRISPR-Based Diagnostics: From Method to Device Journal of Functional Biomaterials CRISPR/Cas molecular diagnostic point of care DNA detection |
| title | Towards Point of Care CRISPR-Based Diagnostics: From Method to Device |
| title_full | Towards Point of Care CRISPR-Based Diagnostics: From Method to Device |
| title_fullStr | Towards Point of Care CRISPR-Based Diagnostics: From Method to Device |
| title_full_unstemmed | Towards Point of Care CRISPR-Based Diagnostics: From Method to Device |
| title_short | Towards Point of Care CRISPR-Based Diagnostics: From Method to Device |
| title_sort | towards point of care crispr based diagnostics from method to device |
| topic | CRISPR/Cas molecular diagnostic point of care DNA detection |
| url | https://www.mdpi.com/2079-4983/14/2/97 |
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