<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
The androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examine...
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MDPI AG
2021-11-01
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| author | Yali Lu Xuechao Wan Wenhua Huang Lu Zhang Jun Luo Dujian Li Yan Huang Yao Li Yaoting Xu |
| author_facet | Yali Lu Xuechao Wan Wenhua Huang Lu Zhang Jun Luo Dujian Li Yan Huang Yao Li Yaoting Xu |
| author_sort | Yali Lu |
| collection | DOAJ |
| description | The androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examines the function and mechanisms of action of lncRNA <i>AC016745.3</i> in the development of PCa. It shows that dihydrotestosterone (DHT) results in the AR-dependent suppression of <i>AC016745.3</i> expression in the LNCaP androgen-sensitive human prostate adenocarcinoma cell line. In addition, overexpression of <i>AC016745.3</i> inhibits the proliferation and migration of PCa cells, and suppresses the expression of AR target genes. This research also demonstrates that the protein NONO interacts with AR and functions as an AR co-activator, promoting AR transcriptional activity. Furthermore, using RNA immunoprecipitation (RIP)-PCR experiments, the study demonstrates that both NONO and AR can bind <i>AC016745.3</i>. Moreover, cell phenotypic experiments reveal that NONO can promote cellular proliferation and migration, and that <i>AC016745.3</i> can partially antagonize the pro-oncogenic functions of NONO in PCa cells. In summary, the results indicate that <i>AC016745.3</i> can bind NONO, suppressing its ability to promote AR-dependent transcriptional activity. Furthermore, DHT-dependent suppression of <i>AC016745.3</i> expression can enhance NONO’s promotion effect on AR. |
| first_indexed | 2024-03-10T05:20:46Z |
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| id | doaj.art-7b223a770eb44a31a300146ebe6735d5 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T05:20:46Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-7b223a770eb44a31a300146ebe6735d52023-11-23T00:04:27ZengMDPI AGLife2075-17292021-11-011111120810.3390/life11111208<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONOYali Lu0Xuechao Wan1Wenhua Huang2Lu Zhang3Jun Luo4Dujian Li5Yan Huang6Yao Li7Yaoting Xu8State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, ChinaDepartment of Urology, Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine, Shanghai 200434, ChinaDepartment of Urology, Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine, Shanghai 200434, ChinaState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, ChinaDepartment of Urology, Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine, Shanghai 200434, ChinaThe androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examines the function and mechanisms of action of lncRNA <i>AC016745.3</i> in the development of PCa. It shows that dihydrotestosterone (DHT) results in the AR-dependent suppression of <i>AC016745.3</i> expression in the LNCaP androgen-sensitive human prostate adenocarcinoma cell line. In addition, overexpression of <i>AC016745.3</i> inhibits the proliferation and migration of PCa cells, and suppresses the expression of AR target genes. This research also demonstrates that the protein NONO interacts with AR and functions as an AR co-activator, promoting AR transcriptional activity. Furthermore, using RNA immunoprecipitation (RIP)-PCR experiments, the study demonstrates that both NONO and AR can bind <i>AC016745.3</i>. Moreover, cell phenotypic experiments reveal that NONO can promote cellular proliferation and migration, and that <i>AC016745.3</i> can partially antagonize the pro-oncogenic functions of NONO in PCa cells. In summary, the results indicate that <i>AC016745.3</i> can bind NONO, suppressing its ability to promote AR-dependent transcriptional activity. Furthermore, DHT-dependent suppression of <i>AC016745.3</i> expression can enhance NONO’s promotion effect on AR.https://www.mdpi.com/2075-1729/11/11/1208prostate cancerandrogen receptor<i>AC016745.3</i>NONOtranscriptional regulation |
| spellingShingle | Yali Lu Xuechao Wan Wenhua Huang Lu Zhang Jun Luo Dujian Li Yan Huang Yao Li Yaoting Xu <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO Life prostate cancer androgen receptor <i>AC016745.3</i> NONO transcriptional regulation |
| title | <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO |
| title_full | <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO |
| title_fullStr | <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO |
| title_full_unstemmed | <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO |
| title_short | <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO |
| title_sort | i ac016745 3 i regulates the transcription of ar target genes by antagonizing nono |
| topic | prostate cancer androgen receptor <i>AC016745.3</i> NONO transcriptional regulation |
| url | https://www.mdpi.com/2075-1729/11/11/1208 |
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