Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments
The discovery of biopigments has received considerable attention from the industrial sector, mainly for potential applications as novel molecules with biological activity, in cosmetics or if aquaculture food supplements. The main objective of this study was to increase the production of carotenoid p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/2/387 |
| _version_ | 1797396592418881536 |
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| author | Alejandra Garcia-Cortes Julián Andres Garcia-Vásquez Yani Aranguren Mauricio Ramirez-Castrillon |
| author_facet | Alejandra Garcia-Cortes Julián Andres Garcia-Vásquez Yani Aranguren Mauricio Ramirez-Castrillon |
| author_sort | Alejandra Garcia-Cortes |
| collection | DOAJ |
| description | The discovery of biopigments has received considerable attention from the industrial sector, mainly for potential applications as novel molecules with biological activity, in cosmetics or if aquaculture food supplements. The main objective of this study was to increase the production of carotenoid pigments in a naturally pigmented yeast by subjecting the yeast to various cellular stresses using design of experiments. The fungal strain <i>Rhodotorula mucilaginosa</i> AJB01 was isolated from a food sample collected in Barranquilla, Colombia, and one of the pigments produced was β-carotene. This strain was subjected to various stress conditions, including osmotic stress using different salts, physical stress by ultraviolet (UV) light, and light stress using different photoperiods. The optimal growth conditions for carotenoid production were determined to be 1 min of UV light, 0.5 mg/L of magnesium sulfate, and an 18:6 h light/dark period, which resulted in a carotenoid yield of 118.3 µg of carotenoid per gram of yeast. |
| first_indexed | 2024-03-09T00:53:37Z |
| format | Article |
| id | doaj.art-7b25430cc3d24a168e13b274896411cc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T00:53:37Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7b25430cc3d24a168e13b274896411cc2023-12-11T17:03:51ZengMDPI AGMicroorganisms2076-26072021-02-019238710.3390/microorganisms9020387Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of ExperimentsAlejandra Garcia-Cortes0Julián Andres Garcia-Vásquez1Yani Aranguren2Mauricio Ramirez-Castrillon3Facultad de Ciencias Básicas, Universidad Santiago de Cali, Campus Pampalinda, Calle 5 # 62-00, Santiago de Cali 760035, ColombiaFacultad de Ciencias Básicas, Universidad Santiago de Cali, Campus Pampalinda, Calle 5 # 62-00, Santiago de Cali 760035, ColombiaFacultad de Ciencias Básicas y Biomédicas, Universidad Simón Bolívar, Carrera 59 # 59-65, Barranquilla 080003, ColombiaFacultad de Ciencias Básicas, Universidad Santiago de Cali, Campus Pampalinda, Calle 5 # 62-00, Santiago de Cali 760035, ColombiaThe discovery of biopigments has received considerable attention from the industrial sector, mainly for potential applications as novel molecules with biological activity, in cosmetics or if aquaculture food supplements. The main objective of this study was to increase the production of carotenoid pigments in a naturally pigmented yeast by subjecting the yeast to various cellular stresses using design of experiments. The fungal strain <i>Rhodotorula mucilaginosa</i> AJB01 was isolated from a food sample collected in Barranquilla, Colombia, and one of the pigments produced was β-carotene. This strain was subjected to various stress conditions, including osmotic stress using different salts, physical stress by ultraviolet (UV) light, and light stress using different photoperiods. The optimal growth conditions for carotenoid production were determined to be 1 min of UV light, 0.5 mg/L of magnesium sulfate, and an 18:6 h light/dark period, which resulted in a carotenoid yield of 118.3 µg of carotenoid per gram of yeast.https://www.mdpi.com/2076-2607/9/2/387Box-Behnken designcarotenoidsβ-carotenecellular stressPlackett-Burman design<i>Rhodotorula</i> |
| spellingShingle | Alejandra Garcia-Cortes Julián Andres Garcia-Vásquez Yani Aranguren Mauricio Ramirez-Castrillon Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments Microorganisms Box-Behnken design carotenoids β-carotene cellular stress Plackett-Burman design <i>Rhodotorula</i> |
| title | Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments |
| title_full | Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments |
| title_fullStr | Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments |
| title_full_unstemmed | Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments |
| title_short | Pigment Production Improvement in <i>Rhodotorula mucilaginosa</i> AJB01 Using Design of Experiments |
| title_sort | pigment production improvement in i rhodotorula mucilaginosa i ajb01 using design of experiments |
| topic | Box-Behnken design carotenoids β-carotene cellular stress Plackett-Burman design <i>Rhodotorula</i> |
| url | https://www.mdpi.com/2076-2607/9/2/387 |
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