Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae
<p>In the past decade much effort has been devoted to the development of new expression systems and novel techniques for the surface display of heterologous proteins in yeast in order to improve their applications in biotechnology, food technology, pharmacology and medicine. Heterologous prote...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Shahid Behehsti University of Medical Sciences
2015-12-01
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| Series: | Applied Food Biotechnology |
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| Online Access: | http://journals.sbmu.ac.ir/afb/article/view/9457 |
| _version_ | 1811276281330794496 |
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| author | Renata Teparic Vladimir Mrsa |
| author_facet | Renata Teparic Vladimir Mrsa |
| author_sort | Renata Teparic |
| collection | DOAJ |
| description | <p>In the past decade much effort has been devoted to the development of new expression systems and novel techniques for the surface display of heterologous proteins in yeast in order to improve their applications in biotechnology, food technology, pharmacology and medicine. Heterologous protein-encoding genes are generally fused with genes coding for yeast cell wall proteins or their fragments required for anchoring. The variety of reactions by which a protein can be displayed at the cell surface enables finding the appropriate one for each individual protein. However, it is still challenging how to improve the efficiency of display of protein complexes and increase the quantity of protein displayed on the yeast surface. Recently, synthetic protein chimeras that self-assemble into the scaffolds on the yeast surface displaying different proteins have been constructed. This review focuses on systems and techniques for display of recombinant proteins on the yeast cell surfaces and applications afforded by this technology.</p> |
| first_indexed | 2024-04-12T23:53:42Z |
| format | Article |
| id | doaj.art-7b27140157494550a5611249d51d6c89 |
| institution | Directory Open Access Journal |
| issn | 2345-5357 2423-4214 |
| language | English |
| last_indexed | 2024-04-12T23:53:42Z |
| publishDate | 2015-12-01 |
| publisher | Shahid Behehsti University of Medical Sciences |
| record_format | Article |
| series | Applied Food Biotechnology |
| spelling | doaj.art-7b27140157494550a5611249d51d6c892022-12-22T03:11:36ZengShahid Behehsti University of Medical SciencesApplied Food Biotechnology2345-53572423-42142015-12-01313145946Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiaeRenata Teparic0Vladimir Mrsa1Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, CroatiaLaboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia<p>In the past decade much effort has been devoted to the development of new expression systems and novel techniques for the surface display of heterologous proteins in yeast in order to improve their applications in biotechnology, food technology, pharmacology and medicine. Heterologous protein-encoding genes are generally fused with genes coding for yeast cell wall proteins or their fragments required for anchoring. The variety of reactions by which a protein can be displayed at the cell surface enables finding the appropriate one for each individual protein. However, it is still challenging how to improve the efficiency of display of protein complexes and increase the quantity of protein displayed on the yeast surface. Recently, synthetic protein chimeras that self-assemble into the scaffolds on the yeast surface displaying different proteins have been constructed. This review focuses on systems and techniques for display of recombinant proteins on the yeast cell surfaces and applications afforded by this technology.</p>http://journals.sbmu.ac.ir/afb/article/view/9457Genetic immobilization, Heterologous protein, Surface display, Yeast cell wall |
| spellingShingle | Renata Teparic Vladimir Mrsa Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae Applied Food Biotechnology Genetic immobilization, Heterologous protein, Surface display, Yeast cell wall |
| title | Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae |
| title_full | Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae |
| title_fullStr | Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae |
| title_full_unstemmed | Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae |
| title_short | Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae |
| title_sort | overview of systems and techniques for surface display of recombinant proteins in yeast s cerevisiae |
| topic | Genetic immobilization, Heterologous protein, Surface display, Yeast cell wall |
| url | http://journals.sbmu.ac.ir/afb/article/view/9457 |
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