Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>)
Several studies in fish have shown that aflatoxin B1 (AFB1) causes a disparity of species-dependent physiological disorders without compromising survival. We studied the effect of dietary administration of AFB1 (2 mg AFB1 kg<sup>−1</sup> diet) in gilthead seabream (<i>Sparus aurata...
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MDPI AG
2021-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/753 |
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| author | Andre Barany Juan Fuentes Gonzalo Martínez-Rodríguez Juan Miguel Mancera |
| author_facet | Andre Barany Juan Fuentes Gonzalo Martínez-Rodríguez Juan Miguel Mancera |
| author_sort | Andre Barany |
| collection | DOAJ |
| description | Several studies in fish have shown that aflatoxin B1 (AFB1) causes a disparity of species-dependent physiological disorders without compromising survival. We studied the effect of dietary administration of AFB1 (2 mg AFB1 kg<sup>−1</sup> diet) in gilthead seabream (<i>Sparus aurata</i>) juveniles in combination with a challenge by stocking density (4 vs. 40 g L<sup>−1</sup>). The experimental period duration was ten days, and the diet with AFB1 was administered to the fish for 85 days prior to the stocking density challenge. Our results indicated an alteration in the carbohydrate and lipid metabolites mobilization in the AFB1 fed group, which was intensified at high stocking density (HSD). The CT group at HSD increased plasma cortisol levels, as expected, whereas the AFB1-HSD group did not. The <i>star</i> mRNA expression, an enzyme involved in cortisol synthesis in the head kidney, presented a ninefold increase in the AFB1 group at low stocking density (LSD) compared to the CT-LSD group. Adenohypophyseal <i>gh</i> mRNA expression increased in the AFB1-HSD but not in the CT-HSD group. Overall, these results confirmed that chronic AFB1 dietary exposure alters the adequate endocrinological physiological cascade response in <i>S. aurata</i>, compromising the expected stress response to an additional stressor, such as overcrowding. |
| first_indexed | 2024-03-10T13:24:13Z |
| format | Article |
| id | doaj.art-7b297ebd298f422db2d2bf75d3e57055 |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:24:13Z |
| publishDate | 2021-03-01 |
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| series | Animals |
| spelling | doaj.art-7b297ebd298f422db2d2bf75d3e570552023-11-21T09:46:51ZengMDPI AGAnimals2076-26152021-03-0111375310.3390/ani11030753Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>)Andre Barany0Juan Fuentes1Gonzalo Martínez-Rodríguez2Juan Miguel Mancera3Department of Biology, Faculty of Marine and Environmental Sciences, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI MAR), University of Cádiz, Puerto Real, 11519 Cádiz, SpainCentre of Marine Sciences (CCMar), Universidade do Algarve, Gambelas, 8005-139 Faro, PortugalInstituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), Puerto Real, 11519 Cádiz, SpainDepartment of Biology, Faculty of Marine and Environmental Sciences, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI MAR), University of Cádiz, Puerto Real, 11519 Cádiz, SpainSeveral studies in fish have shown that aflatoxin B1 (AFB1) causes a disparity of species-dependent physiological disorders without compromising survival. We studied the effect of dietary administration of AFB1 (2 mg AFB1 kg<sup>−1</sup> diet) in gilthead seabream (<i>Sparus aurata</i>) juveniles in combination with a challenge by stocking density (4 vs. 40 g L<sup>−1</sup>). The experimental period duration was ten days, and the diet with AFB1 was administered to the fish for 85 days prior to the stocking density challenge. Our results indicated an alteration in the carbohydrate and lipid metabolites mobilization in the AFB1 fed group, which was intensified at high stocking density (HSD). The CT group at HSD increased plasma cortisol levels, as expected, whereas the AFB1-HSD group did not. The <i>star</i> mRNA expression, an enzyme involved in cortisol synthesis in the head kidney, presented a ninefold increase in the AFB1 group at low stocking density (LSD) compared to the CT-LSD group. Adenohypophyseal <i>gh</i> mRNA expression increased in the AFB1-HSD but not in the CT-HSD group. Overall, these results confirmed that chronic AFB1 dietary exposure alters the adequate endocrinological physiological cascade response in <i>S. aurata</i>, compromising the expected stress response to an additional stressor, such as overcrowding.https://www.mdpi.com/2076-2615/11/3/753<i>sparidae</i>stressaquaculturestocking densityendocrine-related genesenergetic metabolism |
| spellingShingle | Andre Barany Juan Fuentes Gonzalo Martínez-Rodríguez Juan Miguel Mancera Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>) Animals <i>sparidae</i> stress aquaculture stocking density endocrine-related genes energetic metabolism |
| title | Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>) |
| title_full | Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>) |
| title_fullStr | Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>) |
| title_full_unstemmed | Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>) |
| title_short | Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (<i>Sparus aurata</i>) |
| title_sort | aflatoxicosis dysregulates the physiological responses to crowding densities in the marine teleost gilthead seabream i sparus aurata i |
| topic | <i>sparidae</i> stress aquaculture stocking density endocrine-related genes energetic metabolism |
| url | https://www.mdpi.com/2076-2615/11/3/753 |
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