Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways
Exocarpium Citri Grandis (ECG) is used as an antitussive and anti-inflammatory agent in traditional Chinese medicine. However, the mechanism of its anti-inflammatory effect remains unknown. In this study, flavonoids of ECG (FECG) were extracted to investigate the anti-inflammatory effect using a RAW...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
|
| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1550056 |
| _version_ | 1819327114072031232 |
|---|---|
| author | Ying Peng Mengjun Hu Qi Lu Yan Tian Wanying He Liang Chen Kexing Wang Siyi Pan |
| author_facet | Ying Peng Mengjun Hu Qi Lu Yan Tian Wanying He Liang Chen Kexing Wang Siyi Pan |
| author_sort | Ying Peng |
| collection | DOAJ |
| description | Exocarpium Citri Grandis (ECG) is used as an antitussive and anti-inflammatory agent in traditional Chinese medicine. However, the mechanism of its anti-inflammatory effect remains unknown. In this study, flavonoids of ECG (FECG) were extracted to investigate the anti-inflammatory effect using a RAW264.7 macrophage model. The results suggested that FECG could remarkably inhibit the production of nitric oxide (NO). Meanwhile, FECG decreased the mRNA and protein expression of inflammatory cytokines including tumour necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), while showed an opposite effect on interleukin-10 (IL-10). Furthermore, FECG suppressed the phosphorylation of p38 (p38 kinase), JNK (c-Jun N-terminal kinase), ERK (extracellular signal-regulated kinase) and NF-κB. Taken together, our results indicated that FECG might regulate the activation of MAPK and NF-κB signalling pathways to exert anti-inflammatory effects. This study provides some insights into the mechanism underlying the anti-inflammatory activity of ECG, which may facilitate a better application of ECG. |
| first_indexed | 2024-12-24T13:05:41Z |
| format | Article |
| id | doaj.art-7b2a2a3303bb4521b14cdf5a6b642519 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-24T13:05:41Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-7b2a2a3303bb4521b14cdf5a6b6425192022-12-21T16:54:01ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-0130156458010.1080/09540105.2018.15500561550056Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathwaysYing Peng0Mengjun Hu1Qi Lu2Yan Tian3Wanying He4Liang Chen5Kexing Wang6Siyi Pan7Huazhong Agricultural UniversityHuazhong Agricultural UniversityResearch Institute of Agricultural Products Processing and Nuclear-Agricultural TechnologyHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityExocarpium Citri Grandis (ECG) is used as an antitussive and anti-inflammatory agent in traditional Chinese medicine. However, the mechanism of its anti-inflammatory effect remains unknown. In this study, flavonoids of ECG (FECG) were extracted to investigate the anti-inflammatory effect using a RAW264.7 macrophage model. The results suggested that FECG could remarkably inhibit the production of nitric oxide (NO). Meanwhile, FECG decreased the mRNA and protein expression of inflammatory cytokines including tumour necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), while showed an opposite effect on interleukin-10 (IL-10). Furthermore, FECG suppressed the phosphorylation of p38 (p38 kinase), JNK (c-Jun N-terminal kinase), ERK (extracellular signal-regulated kinase) and NF-κB. Taken together, our results indicated that FECG might regulate the activation of MAPK and NF-κB signalling pathways to exert anti-inflammatory effects. This study provides some insights into the mechanism underlying the anti-inflammatory activity of ECG, which may facilitate a better application of ECG.http://dx.doi.org/10.1080/09540105.2018.1550056exocarpium citri grandisanti-inflammatoryflavonoidsmapknf-κb |
| spellingShingle | Ying Peng Mengjun Hu Qi Lu Yan Tian Wanying He Liang Chen Kexing Wang Siyi Pan Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways Food and Agricultural Immunology exocarpium citri grandis anti-inflammatory flavonoids mapk nf-κb |
| title | Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways |
| title_full | Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways |
| title_fullStr | Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways |
| title_full_unstemmed | Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways |
| title_short | Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways |
| title_sort | flavonoids derived from exocarpium citri grandis inhibit lps induced inflammatory response via suppressing mapk and nf κb signalling pathways |
| topic | exocarpium citri grandis anti-inflammatory flavonoids mapk nf-κb |
| url | http://dx.doi.org/10.1080/09540105.2018.1550056 |
| work_keys_str_mv | AT yingpeng flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT mengjunhu flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT qilu flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT yantian flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT wanyinghe flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT liangchen flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT kexingwang flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways AT siyipan flavonoidsderivedfromexocarpiumcitrigrandisinhibitlpsinducedinflammatoryresponseviasuppressingmapkandnfkbsignallingpathways |